A kind of purification method of pleurotus eryngii polysaccharide

The technology of a polysaccharide and a purification method of Pleurotus eryngii is applied in the field of purification of Pleurotus eryngii polysaccharide, which can solve the problems of small volume and time-consuming processing of samples, and achieve the effects of improving the yield and purity of polysaccharide, wide application range and good effect.

Active Publication Date: 2020-06-30
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although simpler than ultrafiltration, dialysis is only suitable for laboratory use due to its smaller sample volumes and is time-consuming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Extraction of Pleurotus eryngii crude polysaccharide

[0028] Cut fresh Pleurotus eryngii fruit bodies into 1cm thin slices, dry at 100°C for 30min, after cooling, pour them into a traditional Chinese medicine grinder, and grind them into 20-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:20. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 8000 r / min for 2 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.

[0029] (2) Preparation of immobilized protease

[0030] Take a 100ml beaker, weigh 2g of chitosan powder, add 20ml of 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, stir in a water bath magnetic stirrer at 30°C for 2h, filter to remove glutaraldehyde Dialdehyde solution, mix glutaraldehyde-activated chitosan with 5ml alkaline protease solution (the total enzyme activity is 2000U), stir in a...

Embodiment 2

[0039] (1) Extraction of Pleurotus eryngii crude polysaccharide

[0040] Cut the fresh Pleurotus eryngii mushroom head (that is, the combined part of Pleurotus eryngii and compost) into 1cm thin slices, dry at 100°C for 30min, after cooling, pour it into a traditional Chinese medicine grinder, and grind it into a 100-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:10. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 6000 r / min for 5 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.

[0041] (2) Preparation of immobilized protease

[0042] Take a 100ml beaker, weigh 2g chitosan powder, add 20ml 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, place it in a water bath magnetic stirrer, keep stirring at 30°C for 2h, filter Remove the glutaraldehyde solution, mix glutaraldehyde-activated chitosan with 5ml neutr...

Embodiment 3

[0051] (1) Extraction of Pleurotus eryngii crude polysaccharide

[0052] Cut fresh Pleurotus eryngii and other foreign products into 1cm thin slices, dry at 100°C for 30 minutes, after cooling, pour them into a traditional Chinese medicine grinder, and grind them into 20-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:30. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 5000 r / min for 10 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.

[0053] (2) Preparation of immobilized protease

[0054] Take a 100ml beaker, weigh 2g of chitosan powder, add 20ml of 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, stir in a water bath magnetic stirrer at 30°C for 2h, filter to remove glutaraldehyde Dialdehyde solution, mix glutaraldehyde-activated chitosan with 5ml trypsin solution (total enzyme activity is 2000U), sti...

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PUM

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Abstract

The invention discloses a purification method of pleurotus eryngii polysaccharides and belongs to the fields of food and medicine. The method disclosed by the invention comprises the following steps:adding specific chitin immobilized protease to pleurotus eryngii water extract, degrading macromolecular proteins in the extract into micromolecular peptides or amino acids, then treating the extractby adopting dialysis or ultra-filtration technology, discarding permeating fluid, reserving trapped fluid, and drying the trapped fluid so as to obtain pleurotus eryngii polysaccharide powder with higher purity. The method disclosed by the invention combines the immobilized enzyme technology with the dialysis technology (or ultra-filtration technology) so as to increase the content of crude polysaccharides in pleurotus eryngii aqueous extract. The method can reduce the content of proteins in the pleurotus eryngii aqueous extract from about 25% to 3% or lower, simultaneously remove a lot of low-molecular-weight impurities such as monosaccharides, oligosaccharides, amino acid and micromolecular peptide, as well as improve the purity of the pleurotus eryngii polysaccharides from 45% to 90% orhigher.

Description

technical field [0001] The invention relates to a method for purifying Pleurotus eryngii polysaccharide, which belongs to the fields of food and medicine, and can also be classified into the technical field of edible mushroom deep processing. Background technique [0002] Pleurotus eryngii ( Pleurotus eryngii Quel), also known as Pleurotus chinensis, belongs to the Phylum Fungi, Basidiomycetes, Agaricaceae, Pleurotaceae, and Pleurotus genus. It is a new species of rare edible fungi that has been successfully developed and cultivated in recent years. At present, the production of Pleurotus eryngii in China has developed very rapidly, and has changed from seasonal cultivation to factory cultivation. Pleurotus eryngii cultivation and processing will produce a large number of by-products (such as deformed mushrooms, mushroom feet, etc.), and its yield can reach more than 20% of the total biomass, which can provide sufficient raw materials for the preparation of Pleurotus eryng...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 郑恒光陈君琛翁敏劼汤葆莎吴俐
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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