Vibrio test piece and preparation method thereof

A technology of test strips and vibrio, applied in biochemical equipment and methods, methods based on microorganisms, measurement/inspection of microorganisms, etc., can solve the problem that the opacity of non-woven fabrics affects the counting results, affects the counting results, and the filter holes are too large and other problems to achieve the effects of easy absorption and diffusion, accurate counting, and accelerated diffusion

Inactive Publication Date: 2018-06-29
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The test piece with filter paper as the carrier has too large filter holes, and it is easy to grow bacteria on the upper and lower layers, which affects the counting results; altho

Method used

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  • Vibrio test piece and preparation method thereof

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preparation example Construction

[0034] A preparation method of vibrio test piece, comprising

[0035] Steps for preparing Vibrio medium: fully dissolve peptone, ox gall powder, sodium thiosulfate, ferric citrate, thymol blue, yeast, sodium chloride, bromothymol blue, and sodium deoxycholate in proportion to After pure water is heated in boiling water for 3-8 minutes, take it out, cool to room temperature, adjust the pH value to 9.0-9.3, then add trisodium citrate and sucrose, stir until completely dissolved, then add xanthan gum and stir until completely dissolved to obtain Vibrio culture medium;

[0036] Prepare the hydrogel carrier step: respectively pass xanthan gum and guar gum through a 120-150 mesh screen, mix the xanthan gum and guar gum evenly according to the mass ratio of 1: (1-2), add pure The water is formulated into a gel with a mass concentration of 0.2-0.8% to obtain a hydrogel carrier;

[0037] Steps for making the test piece: Apply the above-mentioned hydrogel carrier evenly in the culture...

Embodiment 1

[0049] A Vibrio test piece, comprising a test piece main body, a Vibrio culture medium and a hydrogel carrier, the Vibrio culture medium comprising the following components calculated in mass percent: 2.2% peptone, 2.0% sucrose, 0.4% ox gall powder %, sodium thiosulfate 0.15%, ferric citrate 0.05-0.12%, thymol blue 0.005%, yeast 1.5%, sodium chloride 1.0%, sodium deoxycholate 0.2%, trisodium citrate 1.0%, bromide Thymol blue 0.004%, xanthan gum 0.3%, the balance of pure water;

[0050] The main body of the test piece includes a transparent plastic base plate and a transparent plastic film sheet with a grid, the transparent plastic base plate is provided with a culture tank, and one side or a corner of the transparent plastic film sheet is glued and fixed on the surface of the transparent plastic base plate , one end of the transparent plastic film far away from the fixed part of the transparent plastic film and the transparent plastic bottom plate extends to the peeling end; ...

Embodiment 2

[0058] A Vibrio test piece, comprising a test piece main body, a Vibrio culture medium and a hydrogel carrier, the Vibrio culture medium comprising the following components calculated in mass percent: 3.0% peptone, 1.8% sucrose, 0.3% ox gall powder %, sodium thiosulfate 1%, ferric citrate 0.1%, thymol blue 0.004%, yeast 1.2%, sodium chloride 1%, sodium deoxycholate 0.15%, trisodium citrate 0.8%, bromothyme Phenol blue 0.003%, xanthan gum 0.2%, pure water balance;

[0059] The main body of the test piece includes a transparent plastic base plate and a transparent plastic film sheet with a grid, the transparent plastic base plate is provided with a culture tank, and one side or a corner of the transparent plastic film sheet is glued and fixed on the surface of the transparent plastic base plate , one end of the transparent plastic film far away from the fixed part of the transparent plastic film and the transparent plastic bottom plate extends to the peeling end;

[0060] The h...

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Abstract

The invention discloses a vibrio test piece, which comprises a test piece body, a vibrio culture medium and a hydrogel carrier. The vibrio culture medium is prepared from the following components in percentage by mass: 2.2 to 3.5 percent of peptone, 1.5 to 2.0 percent of saccharose, 0.2 to 0.4 percent of ox gallbladder powder, 0.08 to 0.15 percent of sodium thiosulfate, 0.05 to 0.12 percent of ferric citrate, 0.002 to 0.005 percent of thymol blue, 1.0 to 1.5 percent of yeast, 0.8 to 1.0 percent of sodium chloride, 0.12 to 0.2 percent of deoxysodium cholate, 0.5 to 1.0 percent of trisodium citrate, 0.002 to 0.004 percent of bromothymol blue, 0.1 to 0.3 percent of xanthan gum, and the balance of pure water, wherein the pH value of the vibrio culture medium is 9.0 to 9.3. The invention also discloses a preparation method of the vibrio test piece. The vibrio test piece provided by the invention is convenient for a water body sample to absorb and diffuse in the culture medium, and providesa favorable growth environment for vibrio.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a vibrio test piece and a preparation method thereof. Background technique [0002] Vibrio is a common bacteria in China's shrimp breeding period. Once the water quality of shrimp ponds deteriorates, it will easily lead to the prevalence of bacterial diseases. If the bacterial content in the aquaculture water can be monitored in advance, such as the number of Vibrio parahaemolyticus and Vibrio alginolyticus, etc., through early detection, early warning, and timely measures, the incidence of Vibrio can be controlled. [0003] Vibrio detection methods have been researched more and more in-depth, among which loop-mediated isothermal amplification (LAMP) technology, PCR (fluorescence quantitative, dual real-time, EMA-PCR, nested PCR, Real-time PCR) detection technology is mainly used In its molecular detection and genotyping research, ELISA method (indirect method, dot ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/20C12R1/63
CPCC12Q1/06C12Q1/20
Inventor 郑森文李其昌陈善真王志花陈克宏刘博奇杨洁王贵平
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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