Vibrio test piece and preparation method thereof
A technology of test strips and vibrio, applied in biochemical equipment and methods, methods based on microorganisms, measurement/inspection of microorganisms, etc., can solve the problem that the opacity of non-woven fabrics affects the counting results, affects the counting results, and the filter holes are too large and other problems to achieve the effects of easy absorption and diffusion, accurate counting, and accelerated diffusion
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[0034] A preparation method of vibrio test piece, comprising
[0035] Steps for preparing Vibrio medium: fully dissolve peptone, ox gall powder, sodium thiosulfate, ferric citrate, thymol blue, yeast, sodium chloride, bromothymol blue, and sodium deoxycholate in proportion to After pure water is heated in boiling water for 3-8 minutes, take it out, cool to room temperature, adjust the pH value to 9.0-9.3, then add trisodium citrate and sucrose, stir until completely dissolved, then add xanthan gum and stir until completely dissolved to obtain Vibrio culture medium;
[0036] Prepare the hydrogel carrier step: respectively pass xanthan gum and guar gum through a 120-150 mesh screen, mix the xanthan gum and guar gum evenly according to the mass ratio of 1: (1-2), add pure The water is formulated into a gel with a mass concentration of 0.2-0.8% to obtain a hydrogel carrier;
[0037] Steps for making the test piece: Apply the above-mentioned hydrogel carrier evenly in the culture...
Embodiment 1
[0049] A Vibrio test piece, comprising a test piece main body, a Vibrio culture medium and a hydrogel carrier, the Vibrio culture medium comprising the following components calculated in mass percent: 2.2% peptone, 2.0% sucrose, 0.4% ox gall powder %, sodium thiosulfate 0.15%, ferric citrate 0.05-0.12%, thymol blue 0.005%, yeast 1.5%, sodium chloride 1.0%, sodium deoxycholate 0.2%, trisodium citrate 1.0%, bromide Thymol blue 0.004%, xanthan gum 0.3%, the balance of pure water;
[0050] The main body of the test piece includes a transparent plastic base plate and a transparent plastic film sheet with a grid, the transparent plastic base plate is provided with a culture tank, and one side or a corner of the transparent plastic film sheet is glued and fixed on the surface of the transparent plastic base plate , one end of the transparent plastic film far away from the fixed part of the transparent plastic film and the transparent plastic bottom plate extends to the peeling end; ...
Embodiment 2
[0058] A Vibrio test piece, comprising a test piece main body, a Vibrio culture medium and a hydrogel carrier, the Vibrio culture medium comprising the following components calculated in mass percent: 3.0% peptone, 1.8% sucrose, 0.3% ox gall powder %, sodium thiosulfate 1%, ferric citrate 0.1%, thymol blue 0.004%, yeast 1.2%, sodium chloride 1%, sodium deoxycholate 0.15%, trisodium citrate 0.8%, bromothyme Phenol blue 0.003%, xanthan gum 0.2%, pure water balance;
[0059] The main body of the test piece includes a transparent plastic base plate and a transparent plastic film sheet with a grid, the transparent plastic base plate is provided with a culture tank, and one side or a corner of the transparent plastic film sheet is glued and fixed on the surface of the transparent plastic base plate , one end of the transparent plastic film far away from the fixed part of the transparent plastic film and the transparent plastic bottom plate extends to the peeling end;
[0060] The h...
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