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Nanofiber membrane for wound healing and preparation method and application thereof

A composite nanofiber, wound healing technology, applied in bandages, absorbent pads, medical science and other directions, can solve problems such as being unsuitable for soft tissue wound healing, achieve good surface physical and chemical properties, reduce inflammatory response, and accelerate wound healing.

Active Publication Date: 2018-07-10
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the above electrospun biofilms involving gelatin and bioceramics, or polycaprolactone and bioceramics, are used in bone tissue or hard tissue formation applications and are not suitable for soft tissue wounds. Medical excipients for healing skin or muscular tissue wounds especially caused by diabetes

Method used

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  • Nanofiber membrane for wound healing and preparation method and application thereof
  • Nanofiber membrane for wound healing and preparation method and application thereof
  • Nanofiber membrane for wound healing and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Preparation of the composite nanofibrous membrane of the present invention for soft tissue wound healing

[0059] Dissolve PCL, gelatin and NAGEL in an appropriate amount of hexafluoroisopropanol (10%-25%, m / v) solvent at the same time, the addition amount of the three components is shown in each group in Table 1, and the magnetic stirring speed is 600rpm / min to obtain a uniform mixed electrospinning solution, and set up a control group A0 at the same time, see Table 1.

[0060] Table 1

[0061] test group

Polycaprolactone, wt%

Gelatin, wt%

NAGEL, wt%

Group A0 (PL)

50％

50％

0

Group A1 (30NAG-PL)

50％

50％

30％

Group A2 (10NAG-PL)

50％

50％

10％

[0062] Wherein, the PL means not containing NAGEL;

[0063] Wherein, the 10NAG-PL means that the content of NAGEL is 10%.

[0064] Wherein, said 30NAG-PL means that the content of NAGEL is 30%.

[0065] The prepared mixed electrospinning solution...

Embodiment 2

[0071] Example 2: Studying the Effect of the Composite Nanofiber Membrane for Soft Tissue Wound Healing on Cell Proliferation During Wound Repair

[0072] Each group of materials prepared in Example 1 was cut into a circular sheet with Φ=10 mm, soaked in 75% alcohol for 30 minutes, washed twice with sterilized PBS, and put into a 48-well plate for later use. Human umbilical vein endothelial cells, human fibroblasts, and human keratinocytes were planted on the sample surface, 6×10 per well 3 The culture medium used was ECM medium with 5% FBS and growth factors, DMEM medium with 10% FBS and 1640 medium with 10% FBS, the culture temperature was 37°C, and the culture atmosphere was 5% CO 2 air, and the medium was changed every two days. The CCK8 method was used to test the proliferation of cells on the surface of the material. After the cells were cultured for 1, 3, and 7 days, CCK8 solution was added to the 48-well plate in the dark, and cultured at 37° C. for another 2-4 hour...

Embodiment 3

[0076] Example 3: Studying the Effect of the Composite Nanofibrous Membrane for Soft Tissue Wound Healing on the Migration and Adhesion of Human Angiogenesis Cells

[0077] Digest the HUVEC cells in the logarithmic phase with trypsin. After digestion, inoculate the cells in a 24-well plate. When the cell density is about 90% at the bottom of the culture dish, gently draw a straight line with a sterile medium pipette tip. , aspirate the culture medium, and wash twice with PBS. A transwell chamber is added to the upper part of the 24-well plate, and the material prepared in Example 1 is added therein, and the culture medium is filled up and the cells are placed in a 5% CO2 incubator for culture. After culturing for 6-8 hours, take pictures under the OLYMPUS microscope, and count the number of migrated cells.

[0078] Add different materials to the bottom of a 24-well cell culture plate equipped with Boyden chambers. Add 600 μL of endothelial cell culture medium containing 10% s...

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Abstract

The invention provides a preparation method of nanofiber membrane. The method adopts polycaprolactone, gelatin and nagelschmidtite biological ceramic as raw materials, and uses a mixed electrospinningtechnique to prepare the composite nanofiber membrane used for wound healing. The composite nanofiber membrane can observably shorten the healing time of soft tissue chronic wounds, especially diabetes mellitus wounds, promote the angiogenesis, epidermis regeneration and collagen formation of wound areas, reduces inflammatory responses of chronic wounds, and has a good application prospect for soft tissue wound healing.

Description

technical field [0001] The present invention relates to a nanofibrous film for soft tissue wound healing and its preparation method and application, in particular to a novel electrospinning material containing bioactive ceramic calcium diorthosilicate phosphate particles (NAGEL) and its preparation method and use, belonging to the technical field of biomaterials. Background technique [0002] In recent years, with the improvement of the global quality of life, the incidence of chronic diseases such as obesity, diabetes and malnutrition has also increased year by year, and the incidence of complications such as pressure ulcers, venous lower extremity ulcers and chronic wounds such as diabetic ulcers has also increased. Significantly increased [Turner N J, et al, Advances in wound care, 2015]. It is estimated that about 400 million people in the world face wound problems caused by different factors [Harding K, et al., Internationalwoundjournal, 2011]. In the United States al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/26A61L15/18A61L15/32A61L15/42A61L15/44A61L15/46
CPCA61L15/18A61L15/26A61L15/32A61L15/425A61L15/44A61L15/46A61L2300/10A61L2300/112A61L2300/406A61L2300/412A61L2300/414A61L2300/426C08L67/04C08L89/06
Inventor 易正芳吕方吴成铁刘明耀
Owner EAST CHINA NORMAL UNIV
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