Preparation method and application of carboxylated polystyrene microspheres

Abstract

The invention provides a preparation method and application of carboxylated polystyrene microspheres. The preparation method comprises the steps of: using styrene as monomer and ethanol and 2-methoxyethanol as a mixed solvent, and performing a reaction under the action of initiator, a dispersing agent and carboxylation functional monomer. After the solvent is optimized by using the preparation method, the monodispersed microspheres with uniform size can be prepared simply, and the yield is high; and the preparation method is environmentally friendly and green, and environmental protection is achieved.

Description

technical field

[0001] The invention relates to the technical field of polymer microspheres, in particular to a preparation method and application of carboxylated polystyrene microspheres. Background technique

[0002] Liquid phase chip technology, also known as flow microbead technology, is to combine the soluble substance to be tested in the solution on the optically coded microsphere similar to the size of the cell through the specific affinity reaction between biomolecules. The instrument is a new generation of molecular diagnostic technology platform for simultaneous rapid qualitative and quantitative analysis of multiple components in the same trace sample. In European and American countries, liquid-phase chip technology, as an emerging diagnostic technology with superior performance, is constantly developing new clinical diagnostic applications, including pathogen detection, human leukocyte antigen (HLA) typing detection, cytokine detection, etc. The carrier of the l...

Examples

Embodiment 1

[0030] This embodiment provides a kind of preparation method of carboxylated polystyrene microspheres, comprising:

[0031] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was reacted at 45° C. for 20 minutes and then at 70° C. for 24 hours under a nitrogen atmosphere. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 96%, the particle size of the obtained carboxylated polystyrene microspheres is 9 μm, and the dispersion coefficient is 0.011. The SEM image of the carboxylated polystyrene microspheres that the present embodiment obtains is as follows figure 1 .

Embodiment 2

[0033] This embodiment provides a kind of preparation method of carboxylated polystyrene microspheres, comprising:

[0034] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was first reacted at 50° C. for 10 minutes in a nitrogen atmosphere, and then reacted at 80° C. for 20 hours. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 93%, the average particle diameter of the obtained carboxylated polystyrene microspheres is 8.5 μm, and the dispersion coefficient is 0.015.

Embodiment 3

[0036] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was first reacted at 55° C. for 5 minutes, and then reacted at 80° C. for 36 hours under a nitrogen atmosphere. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 96%, the average particle diameter of the obtained carboxylated polystyrene microspheres is 9.5 μm, and the dispersion coefficient is 0.023.

technical field

[0001] The invention relates to the technical field of polymer microspheres, in particular to a preparation method and application of carboxylated polystyrene microspheres. Background technique

[0002] Liquid phase chip technology, also known as flow microbead technology, is to combine the soluble substance to be tested in the solution on the optically coded microsphere similar to the size of the cell through the specific affinity reaction between biomolecules. The instrument is a new generation of molecular diagnostic technology platform for simultaneous rapid qualitative and quantitative analysis of multiple components in the same trace sample. In European and American countries, liquid-phase chip technology, as an emerging diagnostic technology with superior performance, is constantly developing new clinical diagnostic applications, including pathogen detection, human leukocyte antigen (HLA) typing detection, cytokine detection, etc. The carrier of the l...

Examples

Embodiment 1

[0030] This embodiment provides a kind of preparation method of carboxylated polystyrene microspheres, comprising:

[0031] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was reacted at 45° C. for 20 minutes and then at 70° C. for 24 hours under a nitrogen atmosphere. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 96%, the particle size of the obtained carboxylated polystyrene microspheres is 9 μm, and the dispersion coefficient is 0.011. The SEM image of the carboxylated polystyrene microspheres that the present embodiment obtains is as follows figure 1 .

Embodiment 2

[0033] This embodiment provides a kind of preparation method of carboxylated polystyrene microspheres, comprising:

[0034] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was first reacted at 50° C. for 10 minutes in a nitrogen atmosphere, and then reacted at 80° C. for 20 hours. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 93%, the average particle diameter of the obtained carboxylated polystyrene microspheres is 8.5 μm, and the dispersion coefficient is 0.015.

Embodiment 3

[0036] Dissolve 1.5g of dispersant polyvinylpyrrolidone in 27.2g of ethanol and 18.1g of ethylene glycol monomethyl ether, stir at a speed of 200 rpm, then add 0.136g of methacrylic acid and 1wt% azobisiso 13.6 g of styrene butyronitrile was first reacted at 55° C. for 5 minutes, and then reacted at 80° C. for 36 hours under a nitrogen atmosphere. After the reaction, cool to room temperature, take the solid, wash and dry to obtain carboxylated polystyrene microspheres. The yield of this example is 96%, the average particle diameter of the obtained carboxylated polystyrene microspheres is 9.5 μm, and the dispersion coefficient is 0.023.

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