Composition for inhibiting methicillin-resistant staphylococcus aureus biofilm
A methicillin- and staphylococcus-resistant technology is applied in the field of compositions for inhibiting methicillin-resistant Staphylococcus aureus biofilms, which can solve the problems of difficult treatment, persistent infection and easy recurrence, and achieve the effect of enhanced synergy.
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Embodiment 1
[0051] Example 1 Inhibition of methicillin-resistant Staphylococcus aureus biofilm composition test
[0052] Materials and Reagents:
[0053] 1. Carrier:
[0054] Medical silicone sheet (1cm 2 )
[0055] 2. Experimental strains:
[0056] Methicillin-resistant Staphylococcus aureus (MRSA) standard strain: ATCC29213 (provided by the Microbiology Department of the First Affiliated Hospital of Guangxi Medical University)
[0057] Methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate: MRSA 17546 (t037) (identified and provided by the Microbiology Department of the First Affiliated Hospital of Guangxi Medical University).
[0058] 3. Main reagents and drugs
[0059] LB agar, LB broth (Sigma company); absolute ethanol, 75% ethanol, sodium chloride (domestic analytical grade); chloral hydrate (domestic analytical grade); glutaraldehyde (Sigma company), baicalein (Sigma company ), linezolid (Sigma Company), formalin (domestic analytical grade), dimethyl sulfoxide (D...
Embodiment 2
[0110] Embodiment 2 pharmaceutical composition test result
[0111] 1. Gross view of the tissue around the air sac bag of SD rats infected with MRSA-BF:
[0112] Visual view: as Figure 1 to Figure 4 As shown (48 hours after MRSA-BF infection), after the establishment of the infection model, as the infection time prolongs, the inflammatory exudate of the bladder wall of the blank control group gradually increases, but the exudate of each intervention group is less than that of the blank control group, Especially in the baicalein combined with linezolid intervention group, there was almost no exudation of the capsule wall.
[0113] 2. The synergistic effect of baicalein combined with linezolid on the count of viable bacteria in BF on the surface of MRSA-BF infection carrier in SD rats:
[0114]The number of live bacteria in BF on the surface of the carrier in the blank control group, linezolid group, baicalein group and baicalein combined with linezolid rats, CFU (lg10) after...
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