Extraction method and application of Pinus koraiensis pine needle polyphenol compound

A technology of polyphenolic compounds and extraction method, which is applied in the field of extraction of polyphenolic compounds from pine needles of Korean pine, can solve the problems of high toxicity and volatility, unfavorable health of operators, large amount of ionic liquid, etc., and achieves inhibition of α - The effect of glucosidase activity, reducing adverse reactions, and reducing extraction costs

Inactive Publication Date: 2018-07-13
ZAOZHUANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The disadvantages of the above-mentioned patented technology are: 1. All the solid wastes of lignocellulosic biomass are dissolved and extracted by using the mixed solution of ionic liquid, the consumption of ionic liquid is relatively large, and the cost is high; 2. The precipitant contains acetone, It has high toxicity and volatility, which is not conducive to protecting the health of operators
The existing technol...

Method used

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Examples

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Embodiment 1

[0032] The method for extracting polyphenolic compounds from pine needles of Korean pine provided in this embodiment comprises the following steps:

[0033] Step 1: Preparation of degreased pine needles: Clean the pine needles of Korean pine, wash them, dry them at a constant temperature of 60°C for 72 h in a constant temperature blast drying oven, and grind them with a small universal pulverizer after drying is complete. Pharmacopoeia screened for later use. Take 20 g into the feeding tube of the Soxhlet extractor, add 150 mL of petroleum ether for the first time, start timing from the first siphon (70°C), and the reflux extraction time is 1.5 h; add 150 mL of petroleum ether for the second time, from The timing was started at the first siphon (70°C), the reflux extraction time was 1 h, and the defatted pine needle material (about 19.7 g) was obtained.

[0034] Step 2, prepare pine needle polyphenol extract: dissolve 1-butyl-3-methylimidazolium chloride (ionic liquid) in 60%...

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Abstract

The invention relates to the technical field of biomedicine, specifically to an extraction method and application of a Pinus koraiensis pine needle polyphenol compound. The extraction method comprisesfour steps of preparing a defatted pine needle material, preparing pine needle polyphenol extracting liquid, recovering ionic liquid and purifying the pine needle polyphenol. The polyphenol compoundin the pine needles is subjected to auxiliary extraction by ionic liquid, the yield is high, and the effect is good when the Pinus koraiensis pine needle polyphenol compound is applied to preparationof a preparation for inhibiting the activity of alpha-glucosaccharase.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for extracting polyphenolic compounds from pine needles of Korean pine and application thereof. Background technique [0002] Pine needles are the leaves of the gymnosperm Pinaceae Pinus, and are extremely rich in resources in my country. The leaves of Pinus plants are seldom exploited and utilized, resulting in a large amount of waste of resources. The development of domestic pine needle tea is relatively late, and the process is simple. The dried pine needles after initial processing are mainly used for direct tea drinking. Long-term consumption of pine needle tea can play a certain role in lowering blood pressure and blood sugar. Domestic scholars believe that the functional components of pine needles are phenolic acid components, mainly pine needle polyphenols. For the extraction process of pine needle polyphenols, most domestic literatures use a certain volume ...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61P3/10A61K127/00
CPCA61K36/15A61K2236/10A61K2236/13A61K2236/15A61K2236/17A61K2236/333A61K2236/53A61K2236/55
Inventor 王占一
Owner ZAOZHUANG UNIV
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