Collagen as well as preparation method and application thereof

A technology of collagen and protease, applied in the fields of peptide/protein components, chemical instruments and methods, animal/human protein, etc., can solve the problems of uneven beauty effects

Inactive Publication Date: 2018-07-13
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the cosmetic effects of commercially available collagen preparations vary widely. Therefore, it is of great practical significance to provide a collagen preparation with remarkable whitening and moisturizing effects.

Method used

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  • Collagen as well as preparation method and application thereof
  • Collagen as well as preparation method and application thereof
  • Collagen as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Take the cod skin and cut it into pieces with surgical scissors, dry it in an oven at 80°C for 12 hours, then grind it into powder with a pulverizer, and put it in a cool and dry place for later use. Weigh 10g of cod skin powder, add 300mL of water, then adjust the pH to 1.5, raise the temperature of the solution to 40°C, add 0.10g of pepsin to the solution, enzymatically hydrolyze for 6 hours, raise the temperature to 80°C to inactivate the enzyme, and then centrifugally filter the enzymatic solution Obtain the supernatant. The supernatant was spray dried to obtain 2.56 g of collagen. The molecular weight of collagen was determined to be 1000Da~5000Da by high performance size exclusion chromatography.

Embodiment 2

[0046] Take the cod skin and cut it into pieces with surgical scissors, dry it in an oven at 80°C for 12 hours, then grind it into powder with a pulverizer, and put it in a cool and dry place for later use. Weigh 10g of cod skin powder, add 300mL of water, then adjust the pH to 10.5, raise the temperature of the solution to 45°C, add 0.15g of alkaline protease to the solution, enzymolyze it for 8 hours, raise the temperature to 100°C to inactivate the enzyme, and then centrifuge the enzymatic solution The supernatant was obtained by filtration. The supernatant was spray dried to obtain 2.32 g of collagen. The molecular weight of collagen was determined to be 1500Da~5500Da by high performance size exclusion chromatography.

Embodiment 3

[0048]Take the cod skin and cut it into pieces with surgical scissors, dry it in an oven at 80°C for 12 hours, then grind it into powder with a pulverizer, and put it in a cool and dry place for later use. Weigh 10g of cod skin powder, add 500mL of water, then adjust the pH to 2.0, heat the solution to 45°C, add 0.3g pepsin to the solution, enzymolyze for 7.5h, heat up to 100°C to inactivate the enzyme, and then centrifuge the enzymatic solution The supernatant was obtained by filtration. After the supernatant was spray-dried, 2.11 g of collagen was obtained. The molecular weight of collagen was determined to be 1500Da~5500Da by high performance size exclusion chromatography.

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Abstract

The invention relates to the technical field of biology, in particular to collagen as well as a preparation method and application thereof. The collagen provided by the invention can significantly increase skin moisture content of a D-galactose senile-induced nude mouse model; compared with a model control group, the contents of MDA in the skin and animal liver in high and medium dose groups of the collagen are obviously decreased, the content of hydro-xyproline in the skin in the high dose group of the collagen is obviously increased, the contents of SOD in the skin and animal liver in the high dose group of the collagen are obviously increased, and the content of T-AOC in animal plasma in the high dose group of the collagen is significantly increased. The test result shows that the collagen has the effects of resisting oxidation and scavenging free radicals, and has better effects on maintaining skin moisture and improving the content of the collagen in the skin.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to collagen and its preparation method and application. Background technique [0002] In the late 1980s, with the deepening of my country's reform and development policies, people's living standards gradually improved. In addition to having enough food and clothing, people start to pay attention to their external beauty, and often suffer from their own skin problems. Therefore, the demand for beauty care has also grown rapidly in recent years. It is of great practical significance to provide drugs, cosmetics or health food that can improve skin aging. [0003] Skin aging is a complex, multifactorial process that results in both histological and functional damage to the skin. Common manifestations: skin laxity, nodules, leathery appearance, marked dryness, and desquamation. [0004] Dry skin is an uncomfortable feeling of lack of moisture in the skin. The main symptoms are skin tight...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06A61K8/65A61K38/39A61Q19/00A61P17/16
CPCA61K8/65A61K38/39A61Q19/00C07K14/78C12P21/06
Inventor 寇秀颖李文治周勇丁刘刚岳中宝朱华伟
Owner INFINITUS (CHINA) CO LTD
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