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Method for online amplification production of chicken infectious bursal disease viruses

A technology for bursal disease and chicken infectivity, applied in biochemical equipment and methods, viruses, antiviral agents, etc., can solve the problems of high price, unable to maintain stable cell proliferation rate, etc., to reduce residues and facilitate rapid Effects of adhesion, high growth rate

Active Publication Date: 2018-07-13
GUANGZHOU QIZHI BIOLOGICAL ENG EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most disposable microcarriers are expensive, and during the culture process, with the increase of the number of passages, the growth rate of cells on the microcarriers decreases, and a stable cell proliferation rate cannot be maintained during the passage. These factors It has become an obstacle to the application of microcarriers in large-scale cell culture. At the same time, reducing the impact of serum is also a major difficulty in current vaccine production technology

Method used

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  • Method for online amplification production of chicken infectious bursal disease viruses
  • Method for online amplification production of chicken infectious bursal disease viruses
  • Method for online amplification production of chicken infectious bursal disease viruses

Examples

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Embodiment 1

[0032] Embodiment 1 A kind of method of on-line amplified production chicken infectious bursal disease virus

[0033] Embodiment 1 method steps of the present invention are as follows:

[0034] S1. Preparation of seed cells: Take chicken embryo DF-1 cells and carry out spinner bottle culture to obtain seed cells. When the number of cells reaches 1.2×10 10 On-line amplified culture of cells;

[0035] S2. Online amplification and cultivation:

[0036] Online primary amplification: Take the seed cells obtained in step S1 to a 7L bioreactor, add microcarriers 5g / L, culture medium 5L, set the cell attachment parameters as: DO: 50, pH: 7.2, temperature: 37°C, speed: 60 Turn / min, O 2 : 0.2MPa / (L / min) / min, CO 2 : 0.2MPa / (L / min) / min, Air: 0.2MPa / (L / min) / min; switch to cell culture after 3h, the cell culture parameters are: DO: 50, pH: 7.3, temperature: 37℃, Speed: 60 rev / min, O 2 : 0.2MPa / (L / min) / min, CO 2 : 0.2MPa / (L / min) / min, Air: 0.2MPa / (L / min) / min, perfusion flow 3L / day;

[...

Embodiment 2

[0044] Embodiment 2 A kind of method of on-line amplified production chicken infectious bursal disease virus

[0045] The difference between Example 2 and Example 1 is that the cells used in Example 2 are MDCK cells, and the remaining operations and parameters refer to Example 1.

Embodiment 3

[0046] Embodiment 3 A kind of method of on-line amplified production chicken infectious bursal disease virus

[0047] The difference between Example 3 and Example 1 is that the inoculation volume of the virus in Step S3 of Example 3 is 0.05% of the culture volume, and refer to Example 1 for other operations and parameters.

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Abstract

The invention belongs to the technical field of veterinary biological products. The invention particularly relates to a method for online amplification production of chicken infectious bursal diseaseviruses. The method comprises the following steps: S1, preparing seed cells; S2, carrying out tertiary online amplification culture; S3, inoculating viruses; and S4, culturing and harvesting viruses.Compared with the prior art, the method provided by the invention can obviously reduce the production cost, and produce high-titer chicken infectious bursal disease viruses on a large scale, and meanwhile can maintain a higher cell proliferation rate for cells during passage and reduce adverse effects of serum residues on a final product.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a method for on-line amplified production of chicken infectious bursal disease virus. Background technique [0002] Infectious bursal disease (IBD), also known as Gamboreau's disease, is a viral infectious disease caused by infectious bursal virus, which mainly destroys the central immune organ of chickens, the bursa of bursa. Due to the sudden onset of the disease, short course of disease, high infectivity, high mortality, and immunosuppression, it is one of the main infectious diseases in the chicken industry. At present, there is no particularly effective drug for the treatment of chicken infectious bursal disease, and immunization of chickens is the best way to prevent the disease. Chicks can be vaccinated to produce active immunity, and breeder chickens can also be boosted to obtain passive immunity for their offspring, and they will be fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02C12N5/071C12N5/073A61K39/12A61P31/14
CPCA61K39/00C12N5/0603C12N5/0686C12N7/00C12N2531/00C12N2533/70C12N2720/10034C12N2720/10051
Inventor 李祖成尹顺义伍活镰蒋东阳任政华
Owner GUANGZHOU QIZHI BIOLOGICAL ENG EQUIP
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