Bacillus having electrogenic property and denitrification activity and application thereof
A Bacillus and denitrification technology, applied in the field of microbial technology and batteries, can solve the problems of synchronous power generation characteristics and no relevant reports on denitrification activity, so as to improve the broad spectrum of substrates, expand the scope, and efficiently remove nitrate. Effects of nitrogen pollution
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Embodiment 1
[0035] The isolation and identification of embodiment 1 bacterial strain EM-1
[0036](1) Screening and enrichment of bacterial strains: strain EM-1 was screened into the long-term stable operation of the double-chamber MFC system in the laboratory, and the carbon felt with electrogenic activity at the anode was taken out and placed in a sterilized physiological saline solution, and filtered through ultrasonication. Disperses microorganisms which attach to the surface of the anode. Under aseptic conditions, the obtained bacterial suspension was diluted and spread on the solid medium of tryptone soybean broth, and cultured aerobically for 48 hours. According to the difference of bacterial colonies, single colonies were picked and streaked several times. Pure strains were isolated. Further, the pure strain was inoculated into the anolyte solid medium with sodium acetate as the carbon source, and placed in an anaerobic incubator at 30°C for several days, and the colony morpholog...
Embodiment 2
[0045] Example 2 MFC electricity production verification and electrochemical characteristics of bacterial strain EM-1
[0046] MFC starts operation according to (2) and (3) in embodiment 1, and voltage output situation is as follows Figure 4 , after three cycles of operation, the output voltage reached stability in the fourth cycle, which means that the electrochemical activity of the strain EM-1 in the MFC has reached stability, which is considered as a successful startup. After the start-up is successful, it enters the formal operation stage. When the detected output voltage is lower than 20mV, the anolyte is replaced under sterile conditions. Generally, a cycle is about 3 days. Further, after replacing the new anolyte, when the output voltage reaches the highest again and tends to be stable, the MFC polarization curve and power density curve are tested by changing the external resistance, and the results are as follows: Figure 5 As shown, the maximum power density reache...
Embodiment 3
[0047] Example 3 Research on Synchronous Electricity Production and Denitrification Activity of Bacterial Strain EM-1
[0048] MFC starts to run until the voltage output is stable according to (2) and (3) in Example 1, and when the maximum power density reaches Example 2, the fixed sodium acetate concentration in the anolyte component is 1g / L (COD is about 700mg / L ), other inorganic salt components remain unchanged, by changing the concentration of nitrate nitrogen in the anolyte to 0, 20, 50, 100 and 200mg / L respectively, observe the voltage output of different C / N ratio strain EM-1 in MFC and anode denitrification reaction.
[0049] The result is as Figure 6 As shown, with the increase of nitrate nitrogen concentration, the power generation cycle becomes shorter, but the maximum output voltage is almost unchanged. This result means that the electrochemical activity of strain EM-1 is not inhibited by the concentration of nitrate nitrogen, and the main reason for the shorte...
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