A method for fermenting and extracting pharmaceutical grade valine
A valine, pharmaceutical-grade technology, which is applied in the field of fermentation, preparation and extraction of pharmaceutical-grade valine, can solve the problems of difficulty in realizing industrial scale and preparing valine with pharmaceutical-grade purity, etc., so as to prolong the acid production cycle and promote the Utilization and transport, the effect of less residual sugar
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Embodiment 1
[0038] A method for preparing and extracting pharmaceutical grade valine by fermentation, comprising the steps of:
[0039] Brevibacterium lactofermentum ATCC1470 seed liquid (1 × 10 8 cfu / ml) was inserted into the fermentation medium according to the inoculation amount of 7%, and fermented continuously for 55 hours to obtain a valine fermentation liquid; the temperature during the fermentation process was controlled at 30°C, the pH was controlled at 6.5, and the glucose concentration was controlled not less than 20g / L;
[0040] The components of the fermentation medium are: glucose 50g / L, corn steep liquor 30g / L, soybean meal 10g / L, lactose 10g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.1g / L, magnesium sulfate 0.1g / L, manganese sulfate 6mg / L, ferrous sulfate 6mg / L, potassium chloride 6mg / L;
[0041] Utilize the ceramic membrane to filter the valine fermented liquid to obtain filtrate A (valine content is 4.9%) and wet thalline; Add 3wt% tourmaline powder to...
Embodiment 2
[0049] A method for preparing and extracting pharmaceutical grade valine by fermentation, comprising the steps of:
[0050] Brevibacterium lactofermentum ATCC1470 seed liquid (1 × 10 8 cfu / ml) was inserted into the fermentation medium according to the inoculation amount of 8%, and fermented continuously for 50 hours to obtain valine fermentation broth; the temperature during the fermentation process was controlled at 30°C, the pH was controlled at 6.5, and the glucose concentration was controlled not less than 15g / L;
[0051] The components of the fermentation medium are: glucose 50g / L, corn steep liquor 30g / L, soybean meal 10g / L, lactose 10g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.1g / L, magnesium sulfate 0.1g / L, manganese sulfate 6mg / L, ferrous sulfate 6mg / L, potassium chloride 6mg / L;
[0052] Utilize ceramic membrane to filter the valine fermented liquid to obtain filtrate A (valine content is 4.7%) and wet thalline; Add 3wt% tourmaline powder to wet th...
Embodiment 3
[0060] 1. Taking Example 1 as an example, the content of valine was detected, and the content of valine in the filtrate B was detected respectively; a control group was also set at the same time, wherein, the control group 1 was: only dialysis culture treatment was carried out, and no electric Stone powder and heat treatment, the rest are the same as in Example 1; Control group 2: After the fermentation is completed, tourmaline powder and dialysis culture treatment are carried out, without heat treatment, the rest are the same as in Example 1; Control group 3: After the fermentation is completed, heat treatment and dialysis culture treatment are carried out , do not adopt tourmaline powder to process, and all the other are with embodiment 1; Detect the content of valine in each group filtrate B, and concrete results are shown in Table 1:
[0061] Table 1
[0062]
[0063] Conclusion: As shown in Table 1, through the recultivation treatment of discarded bacterial strains, a ...
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