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Preparation method and application of electrochemical biosensor based on gold nanocage

A biosensor, gold nano-cages technology, applied in the field of new functional materials and biosensing detection, can solve the problems of large size of detection equipment, poor selectivity, difficult to popularize, etc., to increase electrode surface area, easy to miniaturize, and low cost Effect

Active Publication Date: 2018-07-31
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection methods currently used in the market have poor selectivity, low sensitivity, high cost, and large detection equipment, which is not easy to promote

Method used

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  • Preparation method and application of electrochemical biosensor based on gold nanocage
  • Preparation method and application of electrochemical biosensor based on gold nanocage
  • Preparation method and application of electrochemical biosensor based on gold nanocage

Examples

Experimental program
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Effect test

Embodiment 1

[0051] This embodiment provides a method for preparing an electrochemical biosensor based on gold nanocages, comprising the following steps:

[0052] (1) Soak the clean base conductive glass sheet in the mercaptosilylating reagent for 12 hours, and dry it with nitrogen;

[0053] (2) A gold nanocage solution with a concentration of 5nmol / L is added dropwise to the base conductive glass sheet obtained in step (1), adsorbed for 4h, rinsed with ultrapure water, and blown dry with nitrogen;

[0054] (3) Place the substrate conductive glass sheet obtained in step (2) in a 2 mg / mL dopamine solution, soak for 4 hours, rinse with ultrapure water, and blow dry with nitrogen;

[0055] (4) Continue to add 5 mg / mL lipase solution dropwise, and leave to stand for 4 hours to prepare an electrochemical sensor.

[0056] Preferably, the preparation of the gold nanocage comprises the following steps:

[0057] (1) Preparation of nano-gold seeds

[0058] Take 42.5 μL of 1% chloroauric acid and ...

Embodiment 2

[0074] This embodiment provides a method for preparing an electrochemical biosensor based on gold nanocages, comprising the following steps:

[0075] (1) Soak the clean base conductive glass sheet in the mercaptosilylating reagent for 12 hours, and dry it with nitrogen;

[0076] (2) A gold nanocage solution with a concentration of 0.1nmol / L is added dropwise to the base conductive glass sheet obtained in step (1), adsorbed for 4h, rinsed with ultrapure water, and blown dry with nitrogen;

[0077] (3) Place the substrate conductive glass sheet obtained in step (2) in a 1 mg / mL dopamine solution, soak for 4 hours, rinse with ultrapure water, and blow dry with nitrogen;

[0078] (4) Continue to add 3 mg / mL lipase solution dropwise, and leave to stand for 4 hours to prepare an electrochemical sensor.

[0079] Preferably, the preparation of the gold nanocage comprises the following steps:

[0080] (1) Preparation of nano-gold seeds

[0081] Take 30 μL of chloroauric acid with a ...

Embodiment 3

[0093] This embodiment provides a method for preparing an electrochemical biosensor based on gold nanocages, comprising the following steps:

[0094] (1) Soak the clean base conductive glass sheet in the mercaptosilylating reagent for 12 hours, and dry it with nitrogen;

[0095] (2) A gold nanocage solution with a concentration of 10nmol / L is added dropwise to the base conductive glass sheet obtained in step (1), adsorbed for 4h, rinsed with ultrapure water, and blown dry with nitrogen;

[0096] (3) Place the substrate conductive glass sheet obtained in step (2) in a 3 mg / mL dopamine solution, soak for 4 hours, rinse with ultrapure water, and blow dry with nitrogen;

[0097] (4) Continue to add 10 mg / mL lipase solution dropwise, and leave to stand for 4 hours to prepare an electrochemical sensor.

[0098] Preferably, the preparation of the gold nanocage comprises the following steps:

[0099] (1) Preparation of nano-gold seeds

[0100] Take 50 μL of 1% chloroauric acid and ...

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Abstract

The invention discloses a preparation method and application of an electrochemical biosensor based on a gold nanocage. A gold-sulfur covalent bond is used for fixing the gold nanocage on a substrate conductive glass sheet immersed in a mercaptosilylating reagent, and electrostatic adsorption force is sequentially utilized for adsorption of dopamine and lipase on the surface of nanoparticles to further modify gold nanocage particles on the substrate conductive glass sheet; the gold nanocage is the gold nanocage particles formed by using a seed growth method to prepare a gold core-silver shell nanocubic structure and finally using chloroauric acid to convert a silver shell under the condition of using cetyltrimethylammonium chloride as a surfactant. The high-performance electrochemical biosensor effectively increases the surface area of an electrode and the electrochemical performance while the maximum biological activity of enzyme is reserved; the high-performance electrochemical biosensor can be used for highly sensitive detection of triglyceride in a water sample and can also be applicable to human serum samples.

Description

technical field [0001] The invention relates to the preparation and application of an electrochemical biosensor based on a gold nanocage, and belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] In recent years, noble metal nanoparticles have shown perfect physical properties due to their suitable size, shape and composition, such as large specific surface area, excellent electrical properties, good biocompatibility, and high catalytic efficiency. Biomolecular carriers can be used to construct the active interface of biosensors, and biosensors with good performance can be prepared. [0003] Among the numerous nanoparticles, gold nanocages are particularly outstanding for their unique optical, electrical, catalytic and other properties, as well as good electrical conductivity. For example: [0004] Chinese patent No. CN104849454A discloses a preparation method and application of an avian herpes virus antigen immu...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 张磊王飞沈晶晶范曲立汪联辉黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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