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Nano drug-delivery process method of graphene oxide and application thereof

A process method, graphene technology, applied in nano-drugs, nano-technology, nano-technology, etc., to achieve the effects of low cytotoxicity, good physiological compatibility, and fast treatment speed

Inactive Publication Date: 2018-08-07
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carriers that are currently studied are liposomes, polymer micelles, solid liposome nanoparticles, dendrimers and carbon nanotubes, and graphene oxide is used as a carrier to apply the drug delivery system of the folate receptor. Not yet reported

Method used

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  • Nano drug-delivery process method of graphene oxide and application thereof
  • Nano drug-delivery process method of graphene oxide and application thereof
  • Nano drug-delivery process method of graphene oxide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of graphene oxide nano drug delivery carrier

[0024] Follow the steps:

[0025] (1) Acidification of graphene oxide (GO): Prepare 1 mol of graphene oxide by Hummers method, add 100 mL of nitric acid, reflux at 120°C for 12 hours, cool and filter, wash the filter residue repeatedly with distilled water, and dry to obtain acidified graphene oxide;

[0026] (2) Synthesis of folic acid-activated lipid (NHS-FA): add 1 mol of folic acid dissolved in ethanol, add condensing agent 3 mol of DCC and 2 mol of NHS in turn, react in the dark at 60°C for 12 hours, stop the reaction, and separate by column chromatography to obtain NHS-FA;

[0027] (3) Synthesis of gelatin-FA conjugates: Dissolve 1.5 mol of gelatin in PB buffer solution with pH=4.5-5.0, add dropwise 1 mol of NHS-FA ethanol solution, then add 1 mol of EDC, and avoid light reaction Overnight, to obtain gelatin-FA conjugates;

[0028] (4) Synthesis of GO-gelatin-FA carrier: Add 1 mol of acidified ...

Embodiment 2

[0029] Example 2 Preparation of doxorubicin-graphene oxide nano drug delivery system

[0030] Add 1 mol of GO-gelatin-FA carrier into water, ultrasonically disperse it evenly, add 5 mol of doxorubicin which has been dissolved in water dropwise, stir with 500W ultrasonic for 30min, react in the dark for 24h, filter, and wash the filter residue repeatedly with distilled water to obtain doxorubicin Su-graphene oxide nano drug delivery system.

Embodiment 3

[0031] Example 3 Preparation of paclitaxel-graphene oxide nano drug delivery system

[0032] Add 1 mol of GO-gelatin-FA carrier into water, ultrasonically disperse it evenly, add 5 mol of paclitaxel dissolved in water dropwise, stir with 500W ultrasonic for 30min, react in the dark for 24h, filter, and wash the filter residue repeatedly with distilled water to obtain paclitaxel-graphite oxide ene nano-drug delivery system.

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Abstract

The invention provides a graphene-oxide nano drug-delivery process method and application thereof. The graphene-oxide nano drug-delivery process method comprises the following steps: acidifying the graphene oxide (GO), synthesizing folic-acid activated fat (NHS-FA), synthesizing gelatin-FA conjugates, synthesizing GO-gelatin-FA carriers, loading drugs and obtaining a graphene oxide nano drug-delivery system. The graphene oxide nano drug-delivery system can be applied to treatment of cancers and has a wide medical application prospect.

Description

technical field [0001] The invention relates to the technical field of nano drug loading, in particular to a process method and application of graphene oxide nano drug loading. Background technique [0002] Graphene oxide (graphene oxide, GO), which is the oxidation product of graphene, contains a large number of hydroxyl, carboxyl and epoxy groups. The introduction of these oxygen-containing active groups not only makes it have good water solubility and stability, Moreover, GO can be more easily modified and functionalized. In addition, both sides of GO have an aromatic structure and a large specific surface area, which can be combined with single-stranded DNA and RNA and aromatic drug molecules such as doxorubicin through non-covalent bonds such as π-π conjugation, hydrogen bonds, and hydrophobic effects. GO is widely used in the field of biomedicine because of its high theoretical loading capacity. [0003] The use of hydrophilic polymer materials to modify GO is a way ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/52A61K47/54A61K47/64A61K47/04A61K47/42A61K47/22A61K31/704A61K31/337A61K31/555A61K31/4745A61P35/00B82Y5/00
CPCA61K47/52A61K31/337A61K31/4745A61K31/555A61K31/704A61K47/02A61K47/22A61K47/42A61K47/545A61K47/6435A61P35/00B82Y5/00
Inventor 赵立春
Owner GUANGXI UNIV OF CHINESE MEDICINE
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