Unlock instant, AI-driven research and patent intelligence for your innovation.

Cultivation method and application of disease-resistant wheat-rye 7BS.7RL chromosomal translocation line

A technology of chromosome translocation and breeding method, which is applied in the field of cultivation of wheat-rye 7BS.7RL chromosome translocation line, and can solve the problems of insufficient stability and low disease resistance of the new translocation line

Inactive Publication Date: 2018-08-10
SICHUAN AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the past 20 years, a large number of new translocation lines have been synthesized, but these new translocation lines are either not stable enough, or have low disease resistance, and cannot be used in production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cultivation method and application of disease-resistant wheat-rye 7BS.7RL chromosomal translocation line
  • Cultivation method and application of disease-resistant wheat-rye 7BS.7RL chromosomal translocation line
  • Cultivation method and application of disease-resistant wheat-rye 7BS.7RL chromosomal translocation line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The source of 7BS.7RL translocation line RT14-245

[0038] The wheat parent of RT14-245 is Mianyang 11 (MY11, 2n=42), a high-yielding wheat variety approved by Sichuan Province in the 1980s, and the rye parent is a rye variety White Grain Rye (2n=14) collected in Southwest China. Mianyang 11 was used as the recipient and white-grained rye as the donor for crossing (Mianyang 11 was multi-generation single-seed self-crossing to obtain a pure genetic background). Genome in situ hybridization proved that Mianyang 11 did not contain any rye chromatin. Since 2000, Mianyang 11 has been highly susceptible to stripe rust (caused by the pathogen Puccinia striiformis f.sp.tritici), powdery mildew (caused by the pathogen Blumeriagraminis f.sp.tritici) and head blight (basically caused by Fusarium graminearum). The F1 generation material after hybridization is a sterile line, so it is treated with 0.05% colchicine and 3% dimethyl sulfoxide for 8 hours at the seedling stag...

Embodiment 2

[0039] Example 2 Molecular cell biology identification of 7BS.7RL

[0040] Using white-grained rye genomic DNA, a repeat clone pAs1 of Goatweed, and rye repeat clone pSc119.2 as probes, and labeled with different fluorescent signals, rye genomic DNA and pAs1 were used Texas red-5- dUTP is marked in red and pSc119.2 is marked in green with Fluor-488-5-dUTP for in situ hybridization. Such as figure 1 and figure 2 As shown, only the wheat chromosome and 7BS.7RL used rye genomic DNA, pAs1 and 119.2 as probes. Rye used pAs1 and 119.2 as probes. From the results of in situ hybridization, it can be clearly seen that the cells contain a pair of 7BS.7RL translocation chromosomes, indicating that RT14-245 is a homozygous 7BS.7RL translocation line.

[0041] The DNA of RT14-245 was identified by PLUG (PCR-based landmark unique gene) molecular marker. The molecular marker TNAC1811 (5'-CTGCTCAACGAGTTCATCGAC-3'; 5'-TTGGAGTGGACGTTGCATT-3') has been proved to be a specific molecular mar...

Embodiment 3

[0043] Embodiment 3 disease resistance identification

[0044] 1. Identification of stripe rust resistance

[0045] As shown in Table 1, the wheat parent Mianyang 11 was highly sensitive to the six physiological races of the stripe rust pathogen used in this experiment, and the rye parent was highly resistant to the six physiological races. Wheat variety Chuannong 10 is a 1RS.1BL translocation line material, carrying the stripe rust resistance gene Yr9, which exists as a control in the disease resistance test and is also highly susceptible to the four stripe rust physiological races. In the field, both Mianyang 11 and Chuannong 10 were highly susceptible to stripe rust. The new material RT14-245 is highly resistant to six kinds of stripe rust pathogens, and also showed high resistance to stripe rust in the field. This shows that RT14-245 is more resistant to stripe rust than the 1RS.1BL translocation line widely used in the world.

[0046] The six physiological races of str...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cultivation method of a disease-resistant wheat-rye 7BS.7RL chromosomal translocation line. The method comprises the following steps: hybridizing Mianyang 11 as a receptor and white-grained rye as a donor to form an F1 generation material, performing chromosome number doubling treatment to form a wheat-rye amphidiploid material, continuously backcrossing with Mianyang 11for twice, selecting a wheat-rye 7R monomer addition line, and performing self-crossing to form the 7BS.7RL translocation line. The invention further discloses a disease-resistant wheat-rye 7BS.7RL chromosomal translocation line through selecting and breeding according to the cultivation method and an application of the translocation line to wheat breeding. The method facilitates transfer and utilization of a novel disease-resistant gene; since 7RL is free from a secaline gene, no negative impact on the wheat processing quality is caused.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a breeding method and application of a disease-resistant wheat-rye 7BS.7RL chromosomal translocation line. Background technique [0002] Wheat is the most important food crop in the world. Stripe rust, powdery mildew and head blight are the three most serious diseases in wheat production, also known as the "cancer" of wheat. In addition to using chemical agents to control production, breeding new strains of wheat resistant to diseases is a more efficient, environmentally friendly and economical method. Due to the narrow genetic base of wheat itself and weak disease resistance, the introduction of excellent disease resistance genes from wheat relative species into wheat through chromosome engineering has become one of the important ways to improve wheat resistance. Among them, the translocation line constructed by chromosomal translocation is the most stable form in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H1/02A01H1/04C12Q1/6813
CPCA01H1/02A01H1/04C12Q1/6813C12Q2563/107
Inventor 任天恒李治任正隆张怀琼谭飞泉唐宗祥符书兰晏本菊
Owner SICHUAN AGRI UNIV