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Regulator for promoting germination of aging rice seeds, and applications thereof

A technology of regulators and rice, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of low germination rate, easy environmental pollution, high irritation, etc., and achieves a simple preparation method, rich raw material sources, The effect of low production cost

Inactive Publication Date: 2018-08-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rice itself is not resistant to storage. Seeds will age for 6 months at room temperature and 3 months at high temperature, resulting in a decline in seed vigor, embryo cell viability decline, low germination rate, slow growth, and even deformity and mildew.
[0004] At present, for the treatment of old rice seeds, chemical reagents such as H2O2 and KMNO4 are mainly used as plant regulators to assist germination. However, this type of reagent is easy to pollute the environment and is highly irritating. If it is not handled properly, it will cause damage to the utensils and the body of the operator.

Method used

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  • Regulator for promoting germination of aging rice seeds, and applications thereof
  • Regulator for promoting germination of aging rice seeds, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This example is a control group. The old rice "Yanfeng 47" stored for one year was disinfected in 1% sodium hypochlorite solution for 30 minutes. Then rinse the old seeds with distilled water until there is no irritating smell, then soak the washed old seeds in distilled water, incubate the distilled water to 28°C before soaking, change the distilled water every 24 hours, and wash the old seeds until there is no sticky feeling . The seed soaking process was kept in darkness at a temperature of 28°C. After soaking the seeds for 3 days, select 80 uniform old seeds and put them on two layers of filter paper in a petri dish. The filter paper is moistened with distilled water. The old seeds are covered with 10 layers of thick gauze, and the gauze is soaked with distilled water. , cultivated in a dark environment, the ambient temperature is 28°C±2°C / 24°C±2°C (14:00 day / 10:00 night), and the air humidity is 70%. Each treatment was repeated 3 times, the filter paper was repl...

Embodiment 2

[0026] This embodiment is the polysaccharide germination group. Put 80 old seeds soaked for 3 days in Example 1 on the double-layer filter paper in the petri dish, the filter paper is moistened with 1 mg / ml polysaccharide solution, and the old seeds are covered with 10 layers of thick gauze, and the gauze is covered with 1 mg / ml polysaccharide solution Wet, as long as there is no excess water in the extrusion, cultivate in a dark environment, the ambient temperature is 28°C±2°C / 24°C±2°C (14:00 day / 10:00 night), and the air humidity is 70%. Each treatment was repeated 3 times, the filter paper was replaced every 2 days, the gauze was supplemented with polysaccharide solution every day to keep it moist, the germination potential was calculated on the 4th day of cultivation, and the germination rate was calculated on the 7th day of cultivation.

Embodiment 3

[0028] This embodiment is the polysaccharide seed soaking-germination acceleration group. The old rice "Yanfeng 47" stored for one year was disinfected in 1% sodium hypochlorite solution for 30 minutes. Then rinse the old seeds with distilled water until there is no pungent smell, then soak the washed old seeds in 1 mg / ml polysaccharide solution, and incubate the solution to 28°C before soaking. The polysaccharide solution was replaced every 24 hours, and the infiltrated old seeds were washed with distilled water until there was no sticky feeling. The seed soaking process was kept in darkness at a temperature of 28°C. After soaking the seeds for 3 days, select 80 uniform old seeds and place them on two layers of filter paper in a petri dish. The filter paper is moistened with polysaccharide solution, and the old seeds are covered with 10 layers of thick gauze. The gauze is soaked with polysaccharide solution to squeeze out no excess water. As the standard, cultivate in a dar...

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Abstract

The invention belongs to the field of plant growth regulators, and particularly relates to a regulator for promoting the germination of aging rice seeds, and applications thereof. According to the present invention, Grateloupia filicina polysaccharide having the total saccharide content of 40-65% and the sulfate content of 20-45% and using galactose as a main component is obtained through hot water leaching, dialysis alcohol precipitation and freeze drying, the 0.05-5 mg / ml Grateloupia filicina polysaccharide is dissolved in water at a temperature of 50-80 DEG C to obtain the regulator for promoting the germination of aging rice seeds, disinfected aging rice seeds are soaked in the regulator for 1-3 days, the soaked aging rice seeds are placed in a culture dish, and infiltration germination is performed; the experiment results show that the germination rate of aging rice seeds can be significantly increased by using the regulator; and the raw material of the regulator is derived from marine red algae, such that the advantages of rich resources, low cost, good effect, natural property and no polluting can be achieved.

Description

technical field [0001] The invention belongs to the technical field of plant growth regulators, and in particular relates to a regulator for promoting the germination of old rice seeds and an application thereof. Background technique [0002] my country is rich in seaweed resources, among which macroalgae are mainly divided into brown algae, green algae and red algae. Centipede algae is a large economical red algae, belonging to the genus Centipede algae of the family Rhodophyta Rhodophyceae Cryptocystaceae. It is a red algae that grows in the intertidal zone and is widely distributed in coastal areas around the world. It belongs to warm temperate seaweed . Polysaccharide is an important product in seaweed, and its dry weight can reach more than 50%. Among red algae polysaccharides, according to their chemical structure, they can be divided into carrageenan type, agar type, and carrageenan-agar intermediate type. According to current reports, Red algae polysaccharides are ...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P21/00A01C1/02
CPCA01C1/02A01N43/16
Inventor 李鹏程刘宏陈晓琳刘松于华华邢荣娥秦玉坤李克成
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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