Compositions comprising lipid cubic crystals of bioactive cosmetic polypeptides and their use in cosmetics
A bioactive, peptide lipid technology, applied in the field of skin repair after microplastic surgery, active cosmetic polypeptide lipid cubic crystal, can solve problems such as damage to the normal structure of the skin and pigmentation
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Embodiment 1
[0057] Example 1. Preparation of Bioactive Cosmetic Polypeptide Lipid Cubic Crystal
[0058] The invention provides a method for preparing lipid cubic crystals of biologically active cosmetic polypeptides, the mass fraction of each component is as follows:
[0059]
[0060] The preparation method is as follows:
[0061] Step 1, dispersing 0.1-10% by weight of stabilizer and 0.1-20% by weight of emulsifier in 1-20% by weight of co-emulsifier to obtain solution A;
[0062] Step 2. Disperse solution A in deionized water, homogenize at a high speed of 5000-1000rpm / min for 2-5min, then add 0-5% by weight of biologically active beauty polypeptide and stir evenly, and let it stand for 10-30min until the reaction is complete to obtain biological Active Beauty Polypeptide Lipid Cubic Crystal ( figure 1 ).
[0063] The bioactive cosmetic polypeptide used therein is EGF. Also, Pluronic F127 was used as a stabilizer, glycerol monooleate was used as an emulsifier, and absolute ethan...
Embodiment 2
[0064] Example 2. Preparation of a composition freeze-dried agent comprising bioactive cosmetic polypeptide lipid cubic crystals
[0065] The present invention provides a preparation method of a composition freeze-dried agent containing biologically active cosmetic polypeptide lipid cubic crystals, the mass fraction of each component is as follows:
[0066]
[0067] The preparation method is as follows:
[0068] Step 1, according to Example 1, prepare the lipid cubic crystal of biologically active cosmetic polypeptide;
[0069] Step 2. Dissolve mannitol and PEG6000 in an appropriate amount of deionized water according to the prescription amount, and mix with trehalose, centella asiatica extract, purslane extract, yeast fermentation lysate filtrate, radish root fermentation product filtrate, grape After the pomelo seed extract and the cubic crystal of biologically active polypeptide lipid are mixed, pre-freeze at -20°C for 120 minutes, and at -80°C for 30 minutes, then vacu...
Embodiment 3
[0071] Embodiment 3. The drug release rate determination of lipid cubic crystal
[0072]Salicylic acid was selected as the detection drug, and lipid cubic crystal samples were prepared according to the method in Example 1. The prepared sample was transferred to a dialysis bag (molecular weight cut-off 8000-10000 Dalton), and the dialysis bag was immersed in 200ml of physiological saline, and magnetically stirred. Detect the absorbance of the salicylic acid solution outside the dialysis bag at 296±1nm in the UV-Vis spectrophotometer at regular intervals, and observe the drug release rate of the lipid cubic crystal. see results image 3 and Figure 4 , image 3 It shows that the absorbance of 0-50ug / ml salicylic acid is linear at 296nm; Figure 4 It shows that the lipid cubic crystal has a sustained release effect on salicylic acid, and the sustained release reaches equilibrium after 6 hours.
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