Neural transplant of three-dimensional orienting structure and preparing method and preparing equipment of neural transplant

A three-dimensional orientation, nerve transplantation technology, applied in the field of nerve grafts and their preparation, can solve the problems of less source of nerve grafts, lesions of the donor site, and incompatibility of the size of the donor nerve, and achieves efficient preparation, good support effect, The effect of high mechanical strength

Active Publication Date: 2018-08-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, autologous nerve transplantation has many limitations, such as: few sources of nerve grafts, donor site lesions, mismatched donor nerve size, and the need for multiple operations, etc.

Method used

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  • Neural transplant of three-dimensional orienting structure and preparing method and preparing equipment of neural transplant
  • Neural transplant of three-dimensional orienting structure and preparing method and preparing equipment of neural transplant
  • Neural transplant of three-dimensional orienting structure and preparing method and preparing equipment of neural transplant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] see figure 1 This embodiment provides a fabrication device (not shown) for preparing a nerve graft with a three-dimensional oriented structure, which includes an electrospinning device (not shown) and a rotating receiving device 10 .

[0065] Wherein, the electrospinning equipment is an existing equipment, and details will not be repeated here. The electrospinning device includes a syringe (not shown) with an injection needle, which is used for jet spinning the polymer solution at the needle in a strong electric field under the action of an electric field. Under the action of an electric field, the droplet at the needle will change from a spherical shape to a conical shape (that is, "Taylor cone"), and extend from the tip of the cone to obtain a polymer filament with a diameter of micron or nanometer.

[0066] The rotating receiving device 10 includes a base 11 , a motor 12 and a turntable 13 , the base 11 and the turntable 13 are detachably connected, and in this embo...

Embodiment 2

[0071] A nerve graft with a three-dimensional oriented structure, which is prepared by using the manufacturing equipment provided in Example 1 by the following method:

[0072] Polylactic acid with an intrinsic viscosity of 3.8 is used as an active ingredient, trifluoroethanol is used as a solvent, a PLLA solution with a concentration of 10% (0.1g / mL) is prepared, and stirred and dissolved at room temperature for 24 hours to obtain a polymer solution, that is, spinning solution.

[0073] Before electrospinning, the air bubbles in the spinning solution were removed by ultrasonic oscillation, and the spinning solution was added to the syringe in the electrospinning equipment, using a national standard No. 9 flat needle.

[0074] Before the electrospinning device is turned on, ethanol is added into the liquid tank as a receiving liquid, wherein the amount of ethanol added is half of the depth of the liquid tank. Then turn on the rotating receiving device, and the motor drives th...

Embodiment 3

[0082] The present invention provides a nerve graft with a three-dimensional oriented structure, which is obtained by using the nerve graft soaked in pure water for use provided in Example 2 and loaded with decellularized hydrogel.

[0083] Specifically, in this example, the decellularized matrix hydrogel is porcine peripheral nerve decellularized matrix hydrogel (pDNM-gel). It is referred to as pDNM for short, and the porcine peripheral nerve decellularized matrix hydrogel is referred to as pDNM-gel for short.

[0084] The porcine peripheral nerve decellularized matrix hydrogel was prepared by the following method:

[0085] 1) Preparation of pDNM:

[0086] The peripheral nerves of pigs were taken from the limbs of male landrace pigs, and the fat tissue and part of the epineurium on the surface were subtracted under the operating microscope. According to the principle of sterility in the whole process of preparation, put the materials into the pre-cooled disinfectant immedia...

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Abstract

The invention relates to a neural transplant of a three-dimensional orienting structure and a preparing method and preparing equipment of the neural transplant, and relates to the technical field of tissue engineering. The preparing method of the neural transplant of the three-dimensional orienting structure comprises the steps of making a polymer solution form fiber through electrostatic spinningand fall into receiving liquid, wherein the receiving liquid directionally flows in a stratum flow state or a transition flow state, and the receiving liquid is selected from cyclohexane and alcoholof any one of C1-C6, preferably methyl alcohol, ethyl alcohol or propyl alcohol. The neural transplant can achieve the effects of mechanically supporting and physically guiding cells and effectively guiding regeneration of nerves. The preparing method of the neural transplant is controllable, convenient and reliable, the preparing equipment of the neural transplant of the three-dimensional orienting structure is manufactured, the structure is simple, and meanwhile, the efficiency of preparing the neural transplant of the three-dimensional orienting structure is effectively improved.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, and in particular to a nerve graft with a three-dimensional orientation structure, a preparation method and a production device thereof. Background technique [0002] With the development of society and economy, the degree of mechanization is increasing day by day, the population mobility is high, and traffic accidents and sports accidents can all cause peripheral nerve injuries. The self-repair ability of human peripheral nerves is limited, and peripheral nerve damage is often permanent. The repair and functional reconstruction of peripheral nerve damage has always been a major problem in clinical research. The clinical treatment of short-distance nerve defects can be sutured end-to-end, but for medium and long-distance nerve defects, nerve grafts are needed to bridge the repair. Currently, the standard for repairing peripheral nerve injuries is autologous nerve grafting. [0003] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/36A61L27/50A61L27/58A61L27/52A61L27/54D01D5/00D01D5/14
CPCA61L27/18A61L27/3633A61L27/3675A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/414A61L2430/32D01D5/0038D01D5/0076D01D5/14C08L67/04
Inventor 全大萍杜钊夷陈诗浩孟月冯文娟饶子龙吴泽佳徐毅炜翟虹蔡绮幸
Owner SUN YAT SEN UNIV
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