Extracellular vesicle-containing injectable hydrogel for treating erectile dysfunction and preparation method of extracellular vesicle-containing injectable hydrogel

A technology for erectile dysfunction and water injection, which is applied in the field of biomedicine, can solve problems such as poor effect and large side effects, and achieve the effects of promoting vascular endothelial repair, nerve regeneration, and recovery of erectile function

Inactive Publication Date: 2018-08-14
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide an injectable hydrogel containing extracellular vesicles for treating erectile dysfunction and a preparation method thereof.

Method used

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  • Extracellular vesicle-containing injectable hydrogel for treating erectile dysfunction and preparation method of extracellular vesicle-containing injectable hydrogel
  • Extracellular vesicle-containing injectable hydrogel for treating erectile dysfunction and preparation method of extracellular vesicle-containing injectable hydrogel
  • Extracellular vesicle-containing injectable hydrogel for treating erectile dysfunction and preparation method of extracellular vesicle-containing injectable hydrogel

Examples

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Embodiment 1

[0028] A medicine for treating erectile dysfunction and a preparation method thereof, comprising the steps of:

[0029] (1) Human mesenchymal stem cell-derived extracellular vesicle extraction: P4 adipose-derived mesenchymal stem cells obtained from the patient's own tissue or cell bank were cultured in clinical-grade human mesenchymal stem cell serum-free medium. When the cell fusion reached 80- At 90%, serum-free DMEM medium was added, and the cell supernatant was collected 24 h later. The cell supernatant was centrifuged at 300 g for 10 min at 4°C, 16,500 g for 10 min at 4°C to remove cells and cell debris, and 120,000 g for 70 min at 4°C to collect extracellular vesicles.

[0030] (2) Quantification of extracellular vesicles derived from human mesenchymal stem cells: The extracellular vesicles derived from human mesenchymal stem cells were diluted 1000 times in phosphate buffer, according to the Brownian motion and diffusion coefficient in the Nanosight instrument (NS300, ...

Embodiment 2

[0037] Example 2 Treatment of Diabetic Erectile Dysfunction in Sprague-Dawley Rats.

[0038] (1) Extracellular vesicles derived from P4 human adipose stem cells were prepared according to the above steps in Example 1 and mixed with p-carboxybenzaldehyde-modified polyethylene glycol-hydroxyethyl chitosan hydrogel to prepare encapsulating human adipose stem cells Injectable hydrogel formulation of p-carboxybenzaldehyde-modified polyethylene glycol-hydroxyethyl chitosan derived from extracellular vesicles.

[0039] (2) A diabetic erectile dysfunction model was established on 8-week-old Sprague-Dawley rats, and 100 μm of the above-mentioned injectable hydrogel preparation encapsulated with human mesenchymal stem cells was injected into the rat corpus cavernosum.

[0040] (3) After 2 weeks of treatment, evaluate the erectile function of the rats, measure the intracavernous pressure (ICP) and mean arterial pressure (MAP), and calculate the ratio of the two.

[0041] Depend on ima...

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Abstract

The invention provides an extracellular vesicle-containing injectable hydrogel for treating an erectile dysfunction. The extracellular vesicle-containing injectable hydrogel for treating the erectiledysfunction comprises an injectable hydrogel and ex vivo human mesenchymal stem cell-derived extracellular vesicles, of which the density is 5*10<10> particles/ml. The invention also provides a methodfor preparing the extracellular vesicle-containing injectable hydrogel for treating the erectile dysfunction. The human mesenchymal stem cell-derived extracellular vesicles can carry bioactivators such as growth factors and nucleic acids to promote vascular repair and nerve regeneration, thereby restoring the erectile function. The invention solves the technical problem of penile vascular endothelium and nerve damage in patients with refractory erectile dysfunction by injecting the human mesenchymal stem cell-derived extracellular vesicles into the corpus cavernosum. The extracellular vesicle-containing injectable hydrogel provided by the invention utilizes the injectable hydrogel to encapsulate the human mesenchymal stem cell-derived extracellular vesicles to provide a safe, effective and convenient therapeutic pharmaceutical preparation for treating the erectile dysfunction.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a medicine for treating erectile dysfunction, in particular to an injectable hydrogel containing extracellular vesicles for treating erectile dysfunction and a preparation method thereof. . Background technique [0002] The current first-line treatment for erectile dysfunction is oral phosphodiesterase-5 inhibitors, such as tadalafil, sildenafil, etc. The main structure of this type of drug is a non-selective or selective phosphodiesterase-5 inhibitor. The principle of treating erectile dysfunction is to increase the concentration of cyclic guanosine monophosphate by inhibiting the activity of penile phosphodiesterase-5 and reducing degradation. Extracellular vesicles are sac-like structures released by cells into the extracellular space during proliferation. According to the diameter, it can be divided into: exosome (diameter: 50-100 nm) and microparticle (microparticle, diameter: 100-...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/06A61K47/10A61K47/36A61P15/10A61P3/10
CPCA61K9/0019A61K9/0034A61K9/06A61K35/28A61K47/10A61K47/36A61P3/10A61P15/10
Inventor 卢慕峻肖冬冬隋晓锋荣立夺张明吕向国燕浩成欢王雅梅
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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