Plant fungicide for prevention and control of rice diseases
A plant fungicide, rice disease technology, applied in the direction of plant growth regulators, chemicals for biological control, biocides, etc., can solve the problem of inability to increase the amount of use, maintain the effect of streptomycin, the environment and human and animal health hazards And other issues
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[0015] 1. In vitro antibacterial activity experiment
[0016] (1) Test method
[0017] Adopt turbidity method, tested the inhibitory activity of each experimental drug to rice bacterial blight (Xanthomonasoryza epv.oryzae (Xoo)), concrete operation steps are as follows:
[0018] A. Add 1000mL sterilized distilled water into a 2000mL beaker, add 5.0g peptone,
[0019] Yeast powder 1.0g, glucose 10.0g, beef extract 3.0g, after stirring evenly, adjust the pH to neutral (7.2±0.2) with sodium hydroxide aqueous solution;
[0020] B. Clean and sterilize the test tubes and put them on the test tube rack. Use a pipette gun to pipette 4.0mL of the solution in step A into each test tube and then add a rubber stopper. Pack each 6 test tubes once. Use a sterilizing pot at 121 Sterilize at ℃ for 20 minutes before use;
[0021] C. Weigh 0.00375-0.0042g sample to be tested in a centrifuge tube, dissolve it with 150μL DMSO, pipette 80μL and 40μL respectively into sterilized numbered centrif...
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