Method for changing genipin cross-linking collagen color development by sunlight
A sunlight-linked collagen technology, applied in medical science, prostheses, etc., can solve the problems of reducing the degree of cross-linking, uncontrollable time, slowness, etc., and achieves good biocompatibility, safe processing methods, and no secondary pollution effect
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Embodiment 1
[0027] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:
[0028] (1) Under the stirring state, in the acidic collagen solution of 7 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 5% by volume;
[0029] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;
[0030] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the collagen surface;
[0031] (4) The blue-black gel was originally exposed to natural light in summer for 6 hours (10:00-16:00), and the color of the gel changed to light blue-gray.
Embodiment 2
[0033] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:
[0034] (1) Under the stirring state, in the acidic collagen solution of 7 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 5% by volume;
[0035] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;
[0036] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the surface;
[0037] (4) After 12 hours of natural light in summer (7:00-19:00), the color of the blue-black gel becomes light brown.
Embodiment 3
[0039] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:
[0040] (1) Under the stirring state, in the acidic collagen solution of 5 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 3% by volume;
[0041] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;
[0042] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the surface;
[0043] (4) The blue-black gel is simulated by the sun light source (the irradiance of the simulated sun light source is 1000W / m 2 , UV irradiance 30W / cm 2 ) light for 6 hours, the color of the gel changed to light brown.
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