Method for Determination of Cannabidiol Content Based on Silver Nano-UV-Vis Spectrophotometry

A spectrophotometry and cannabidiol technology, applied in the field of analytical chemistry, can solve the problems of high requirements on the hardware conditions of instruments and equipment, cumbersome methods, etc., and achieve the effects of low cost, good selectivity and simple instrument requirements

Active Publication Date: 2020-07-31
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these methods, chromatographic methods are required for separation and determination, which is cumbersome and requires high hardware conditions for instruments and equipment.

Method used

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  • Method for Determination of Cannabidiol Content Based on Silver Nano-UV-Vis Spectrophotometry
  • Method for Determination of Cannabidiol Content Based on Silver Nano-UV-Vis Spectrophotometry
  • Method for Determination of Cannabidiol Content Based on Silver Nano-UV-Vis Spectrophotometry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for determining the content of cannabidiol in the extract based on silver nanometer ultraviolet-visible spectrophotometry comprises the following steps:

[0023] (1) Preparation of the standard solution system of cannabidiol: take 9 10mL colorimetric tubes, and add 1.0mL of 0.1% 1-octane sodium sulfonate solution, 1.0mL 0.1g / L of silver nitrate solution, 0.5mL of 0.1g / L of sodium hydroxide solution and 0.5mL of 0.1% ammonia solution by mass percentage, shake them up separately, and then add 1.0mL of cannabis with different concentrations into 9 colorimetric tubes Diphenol standard solution, the concentration of cannabidiol standard solution added in each colorimetric tube is 0.002, 0.004, 0.006, 0.008, 0.010, 0.012, 0.014, 0.016, 0.018mg / mL, after constant volume mixing with distilled water , reacted in a water bath at 50°C for 40min (the transmission electron micrograph of the silver nanoparticles generated by the reaction of 0.018mg / mL cannabidiol standard...

Embodiment 2

[0031] The method for determining the content of cannabidiol in the extract based on silver nanometer ultraviolet-visible spectrophotometry comprises the following steps:

[0032] (1) Preparation of the standard solution system of cannabidiol: take 10 10mL colorimetric tubes, and add 1.0mL of 1.0% sodium 1-octanesulfonate solution, 1.0mL 1.0g / L of silver nitrate solution, 0.5mL of 1.0g / L sodium hydroxide solution and 0.5mL of 1.0% ammonia solution by mass percentage, shake well respectively, and then add 1.0mL of cannabis with different concentrations into 10 colorimetric tubes Diphenol standard solution, the concentrations of the cannabidiol standard solution added in each colorimetric tube are 0.002, 0.004, 0.006, 0.008, 0.010, 0.012, 0.014, 0.016, 0.018, 0.020 mg / mL, mixed with distilled water to volume After homogenization, react in a 90°C water bath for 40 minutes;

[0033] (2) Prepare a blank control system without adding cannabidiol according to the method of step (1)...

Embodiment 3

[0040] The method for determining the content of cannabidiol in the extract based on silver nanometer ultraviolet-visible spectrophotometry comprises the following steps:

[0041] (1) Preparation of standard solution system of cannabidiol: take 10 10mL colorimetric tubes, and add 1.0mL of 0.2% 1-octane sodium sulfonate solution, 1.0mL 0.5g / L of silver nitrate solution, 0.5mL of 0.7g / L of sodium hydroxide solution and 0.5mL of 0.8% ammonia solution by mass percentage, shake them up separately, and then add 1.0mL of cannabis with different concentrations to 10 colorimetric tubes Diphenol standard solution, the concentrations of the cannabidiol standard solution added in each colorimetric tube are 0.002, 0.004, 0.006, 0.008, 0.010, 0.012, 0.014, 0.016, 0.018, 0.020 mg / mL, mixed with distilled water to volume After homogenization, react in a 70°C water bath for 40 minutes;

[0042] (2) Prepare a blank control system without adding cannabidiol according to the method of step (1);...

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Abstract

The invention discloses a method for detecting the content of industrially extracted cannabidiol based on silver nanometer ultraviolet-visible spectrophotometry. The method comprises the following steps: sequentially adding a sodium 1-octanesulfonate solution, a silver nitrate solution, a sodium hydroxide and an aqueous ammonia solution into a colorimetric tube, performing uniform mixing, adding acannabidiol solution, performing constant volume reaching and uniform mixing, and performing a reaction in a water bath; detecting the intensity of an absorption peak at 435 nm on an ultraviolet-visible spectrophotometer; drawing a working curve; and detecting the absorbance of a sample, and calculating the content of the cannabidiol in the extract according to the working curve and a sample solution preparation process.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for measuring the content of industrially extracted cannabidiol based on silver nano-ultraviolet-visible spectrophotometry. Background technique [0002] Cannabidiol is a non-addictive biological component extracted and identified from industrial hemp. Modern biomedical research shows that it can hinder the influence of tetrahydrocannabinol, an addictive component in cannabis, on the human nervous system, and has Antispasmodic, antirheumatic arthritis, antianxiety, antidepressant, anti-insomnia and other pharmacological activities. [0003] In the determination methods of cannabidiol content, the existing reports include gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), gas chromatography (GC-FID), high performance liquid chromatography method (HPLC), etc. All of these methods require the use of chromatographi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/38
CPCG01N1/38G01N21/3103
Inventor 王齐李晓蕾杨俊刘春侠周荣锋贺与平袁锐波
Owner KUNMING UNIV OF SCI & TECH
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