A compound enzyme preparation for enzymolysis of chicken liver and its application
A technology of compound enzyme preparation and chicken liver, which is applied in the field of compound enzyme preparation for enzymatic hydrolysis of chicken liver, can solve the problems of high content of bitter amino acids in enzymatic hydrolysis products, low utilization rate of chicken liver, and inability to be used on a large scale, so as to improve animal health. The effect of growth performance, saving breeding cost and improving breeding efficiency
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Embodiment 1
[0047] 1. Each enzyme activity in the compound enzyme preparation needs to meet the following requirements:
[0048] 50,000 U / g≤neutral protease activity≤100,000 U / g; 100,000 U / g≤alkaline protease activity≤20U / g; 50,000 U / g≤lipase activity≤10U / g; 5000U / g≤visceral enzyme activity≤10,000 U / g; 5000U / g≤flavor enzyme activity≤10,000 U / g.
[0049] 2. Particle size of compound enzyme preparation: 80 mesh pass rate ≥ 99.0%.
[0050] 3. The carrier includes: one or more of corn starch, rice bran, talcum powder, wheat bran, and wheat meal.
[0051] 4. The number of lipase, visceral enzyme, flavor enzyme and carrier in the compound enzyme preparation is fixed, and the neutral protease and alkaline protease are mixed in different proportions to form the compound enzyme preparation.
[0052] (1) Compound enzyme preparation 1:
[0053] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:2:1:0.1:0.2:4;
[0054] (2) Compound enzyme preparation 2:
[...
Embodiment 2
[0087] 1. The number of neutral protease, alkaline protease, lipase, visceral enzyme, and carrier remains unchanged, and the number of flavor enzymes is changed to determine the enzymatic hydrolysis effect of chicken liver, protein molecular weight and flavor amino acid content.
[0088] (1) Compound enzyme preparation 6:
[0089] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.05:4;
[0090] (2) Compound enzyme preparation 7:
[0091] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.10:4;
[0092] (3) Compound enzyme preparation 8:
[0093] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.15:4;
[0094] (4) Compound enzyme preparation 9:
[0095] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.20:4;
[0096] (5) Compound enzyme preparation 10:
[0097] Neutral proteas...
Embodiment 3
[0131] 1. Compound enzyme preparation 3:
[0132] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.1:0.2:4.
[0133] 2. Additive amount, set 5 gradients: 0.50%, 1.00%, 1.50%, 2.00%, 2.50%;
[0134] 3. Using chicken liver as the raw material, the compound enzyme preparation 3 is at the optimum pH 8.0, the enzymolysis temperature is 50°C, raw material: water = 4:1, and the enzymolysis time is 2 hours.
[0135] 4. Determination indicators: total protein nitrogen, amino nitrogen, protein hydrolysis degree, nitrogen recovery rate.
[0136] 5. Determination method:
[0137] Total protein nitrogen: Kjeldahl method;
[0138] Amino nitrogen: formaldehyde potentiometric titration;
[0139] Degree of proteolysis (DH, %) = (mass of amino nitrogen in the supernatant / mass of total nitrogen in the sample) × 100%;
[0140] Nitrogen recovery rate (NR, %)=(amino nitrogen content in the supernatant / total nitrogen content in the raw material)×100...
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