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A compound enzyme preparation for enzymolysis of chicken liver and its application

A technology of compound enzyme preparation and chicken liver, which is applied in the field of compound enzyme preparation for enzymatic hydrolysis of chicken liver, can solve the problems of high content of bitter amino acids in enzymatic hydrolysis products, low utilization rate of chicken liver, and inability to be used on a large scale, so as to improve animal health. The effect of growth performance, saving breeding cost and improving breeding efficiency

Active Publication Date: 2020-11-10
吴广兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings and deficiencies in the prior art that the utilization rate of chicken liver is not high, the enzymatic hydrolysis product has high content of bitter amino acids and heavy fishy smell, and cannot be used on a large scale, the primary purpose of the present invention is to provide a compound enzyme preparation for enzymatic hydrolysis of chicken liver

Method used

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  • A compound enzyme preparation for enzymolysis of chicken liver and its application
  • A compound enzyme preparation for enzymolysis of chicken liver and its application
  • A compound enzyme preparation for enzymolysis of chicken liver and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Each enzyme activity in the compound enzyme preparation needs to meet the following requirements:

[0048] 50,000 U / g≤neutral protease activity≤100,000 U / g; 100,000 U / g≤alkaline protease activity≤20U / g; 50,000 U / g≤lipase activity≤10U / g; 5000U / g≤visceral enzyme activity≤10,000 U / g; 5000U / g≤flavor enzyme activity≤10,000 U / g.

[0049] 2. Particle size of compound enzyme preparation: 80 mesh pass rate ≥ 99.0%.

[0050] 3. The carrier includes: one or more of corn starch, rice bran, talcum powder, wheat bran, and wheat meal.

[0051] 4. The number of lipase, visceral enzyme, flavor enzyme and carrier in the compound enzyme preparation is fixed, and the neutral protease and alkaline protease are mixed in different proportions to form the compound enzyme preparation.

[0052] (1) Compound enzyme preparation 1:

[0053] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:2:1:0.1:0.2:4;

[0054] (2) Compound enzyme preparation 2:

[...

Embodiment 2

[0087] 1. The number of neutral protease, alkaline protease, lipase, visceral enzyme, and carrier remains unchanged, and the number of flavor enzymes is changed to determine the enzymatic hydrolysis effect of chicken liver, protein molecular weight and flavor amino acid content.

[0088] (1) Compound enzyme preparation 6:

[0089] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.05:4;

[0090] (2) Compound enzyme preparation 7:

[0091] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.10:4;

[0092] (3) Compound enzyme preparation 8:

[0093] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.15:4;

[0094] (4) Compound enzyme preparation 9:

[0095] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.10:0.20:4;

[0096] (5) Compound enzyme preparation 10:

[0097] Neutral proteas...

Embodiment 3

[0131] 1. Compound enzyme preparation 3:

[0132] Neutral protease: alkaline protease: lipase: visceral enzyme: flavor enzyme: carrier = 1:4:1:0.1:0.2:4.

[0133] 2. Additive amount, set 5 gradients: 0.50%, 1.00%, 1.50%, 2.00%, 2.50%;

[0134] 3. Using chicken liver as the raw material, the compound enzyme preparation 3 is at the optimum pH 8.0, the enzymolysis temperature is 50°C, raw material: water = 4:1, and the enzymolysis time is 2 hours.

[0135] 4. Determination indicators: total protein nitrogen, amino nitrogen, protein hydrolysis degree, nitrogen recovery rate.

[0136] 5. Determination method:

[0137] Total protein nitrogen: Kjeldahl method;

[0138] Amino nitrogen: formaldehyde potentiometric titration;

[0139] Degree of proteolysis (DH, %) = (mass of amino nitrogen in the supernatant / mass of total nitrogen in the sample) × 100%;

[0140] Nitrogen recovery rate (NR, %)=(amino nitrogen content in the supernatant / total nitrogen content in the raw material)×100...

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Abstract

The invention discloses a composite enzyme preparation for chicken liver enzymolysis and application thereof, and belongs to the field of composite enzyme preparations. The composite enzyme preparation is prepared from the following ingredients in parts by mass: 1 to 3 parts of neutral protease, 1 to 6 parts of alkaline protease, 1 to 3 parts of lipase, 0.1 to 0.3 part of internal organ enzymes, 0.1 to 0.4 part of flavor enzymes and 3 to 5 parts of carriers. The neutral protease, the alkaline protease, the lipase, the internal organ enzymes, the flavor enzymes and the carriers are compounded into the composite enzyme preparation according to different enzyme activities, proportioning ratios and doses; the chicken liver is subjected to enzymolysis; the product protein hydrolysis degree andnitrogen recovery rate are higher; the amino acid molecular weight is small; the bitter amino acid is few; the palatability is high; very high nutrition values are realized. The enzymolysis product issubjected to settlement and freeze drying to be further prepared into chicken liver protein powder; the protein content is high; the chicken liver protein powder replaces partial fish meal to be usedfor feeding animals; the animal growth performance can be improved; the culture benefits can be improved; the culture cost is reduced.

Description

technical field [0001] The invention belongs to the field of compound enzyme preparations, in particular to a compound enzyme preparation for enzymolyzing chicken liver and its application. Background technique [0002] In recent years, my country's chicken production has steadily increased. In 2013, the total production ranked second in the world, becoming the second largest meat product category after pork in per capita consumption, and chicken liver, a by-product of chicken, accounts for 2% of each chicken. ~2.5%, and the annual output reaches more than 200,000 tons. Chicken liver has high nutritional value and contains protein, fat, carbohydrates, vitamin A, vitamin D, phosphorus and other ingredients. It is rich in iron and can nourish blood. In addition, the content of vitamin A in chicken liver is much higher than that of milk, eggs, meat, fish and other conventional foods. It has the function of maintaining normal growth and reproduction, and at the same time can pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50C12N9/20A23K10/14A23K20/147A23K20/142A23K50/60A23K50/30
CPCA23K10/14A23K20/142A23K20/147A23K50/30A23K50/60C12N9/20C12N9/50C12Y301/01003
Inventor 吴广兵龚俊勇
Owner 吴广兵