Tagetes erecta fermenting method

A treatment method, marigold technology, applied in the direction of fermentation, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of cumbersome steps, underutilized lactic acid, waste, etc., and achieve the effect of ensuring the extraction rate

Active Publication Date: 2018-09-04
焉耆晨晓农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Marigold produces a large amount of organic acids during the fermentation process, and these organic acids are directly discharged along with the wastewater, causing a lot of waste
[0004] The patent document with publication number CN107629481A discloses the use of lactic acid bacteria to ferment marigolds, but it uses two fermentations, the steps are cumbersome, and the lactic acid produced by fermentation is not fully utilized, and it is discharged together with waste water, causing waste

Method used

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  • Tagetes erecta fermenting method

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preparation example Construction

[0028] The preparation method comprises the following steps:

[0029]A. Preparation of lactic acid bacteria liquid: Weigh 5g lactose, 20g glucose, 10g peptone, 10g beef extract, 5g yeast powder, 1mL Tween-80, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 2g ammonium citrate, 0.2 g of magnesium sulfate and 0.05 g of manganese sulfate are added to 1L of water, and sterilized by high-pressure steam at 110-120°C for 25-40 minutes to obtain a liquid enrichment medium, and then inoculate the liquid enrichment medium with a mass percentage of 1- 5% lactic acid bacteria strains, cultivated at 25-40°C, the number of effective viable bacteria after cultivation is at least 10 billion / mL.

[0030] B. Fermentation: First, pre-mix the lactic acid bacteria liquid, calcium source compound and marigold flower raw materials according to the mass ratio of 1: (20-100): (200-800) to obtain the pre-mixed raw materials, and then spread them on the bottom of the fermentation tank One layer o...

Embodiment 1

[0033] A method for fermenting marigolds, comprising the process of fermenting marigolds with microorganisms, the fermentation raw materials include marigold flowers, calcium source compounds and lactic acid bacteria liquid, and at the same time, fermented exudates containing calcium lactate and chrysanthemum residues are obtained.

[0034] The preparation method comprises the following steps:

[0035] A. Preparation of lactic acid bacteria liquid: Weigh 5g lactose, 20g glucose, 10g peptone, 10g beef extract, 5g yeast powder, 1mL Tween-80, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 2g ammonium citrate, 0.2 g of magnesium sulfate and 0.05 g of manganese sulfate were added to 1L of water, and sterilized by high-pressure steam at 110°C for 25 minutes to obtain a liquid enrichment medium, and then inoculate the liquid enrichment medium with 1% Layse milk Bacillus (Lactobacillus leichmannii) strain, cultivated at 25°C, the effective number of viable bacteria after cultiv...

Embodiment 2～5

[0039] Embodiment 2~5 is identical with above-mentioned production process step, and difference is process parameter, or the fermented bacterial classification of choosing, as shown in table 1 below, wherein, lactic acid bacteria bacterium liquid: calcium carbonate: marigold fresh flower is each material The mass ratio of marigold flowers spread at the bottom of the fermentation tank: the premixed raw material is the height ratio of the two substances.

[0040] Table 1

[0041]

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Abstract

The invention discloses a tagetes erecta fermenting method. The method comprises a process for fermenting tagetes erecta by using microorganisms, wherein fermented raw materials includes fresh flowersof tagetes erecta, a calcium-source compound and lactobacillus bacteria solution, obtains a fermented percolate containing calcium lactate and tagetes erecta residue, and used for a production process for simultaneously preparing the calcium lactate and lutein. By adopting the tagetes erecta fermenting method, the yield of lactobacillus can be increased, the reaction time is shortened, the production cost is decreased, the prepared calcium lactate is high in purity, the fermented tagetes erecta residue can be used for extracting the lutein, and the process produces no waste and has no pollution.

Description

technical field [0001] The invention relates to a preparation method of calcium lactate, in particular to a method for producing calcium lactate by microbial fermentation, which is used for the production process of simultaneously preparing calcium lactate and lutein. Background technique [0002] Calcium lactate is widely used in dairy products, beverages, health food, feed, fertilizer and other fields. Conventional production methods mainly include fermentation and neutralization. The fermentation method uses calcium carbonate as a neutralizing agent to carry out lactic acid fermentation to directly generate calcium lactate; the middle method is to use the finished lactic acid solution to add calcium hydroxide or calcium oxide for neutralization reaction. The generation of lactic acid is the key in the preparation process of calcium lactate. Most of the fermentation production of lactic acid uses a large amount of glucose or sucrose as a carbon source, and configures othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12N1/20C12R1/225C12R1/46C12R1/01
CPCC12N1/20C12P7/56
Inventor 卢庆国陈月萍曹养宪韩文杰连运河
Owner 焉耆晨晓农业科技有限公司
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