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A kind of benzene-degrading bacteria for controlling air pollution and its preparation method and application

A technology for degrading bacteria and strains, which is applied in the field of bacterial strains containing benzene series air pollution control, can solve the problems of low degradation effect, limited treatment effect, and difficulty in meeting gas discharge standards, achieving low cost and significant effect

Active Publication Date: 2021-05-14
BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially the benzene series gas in the waste gas is more toxic. The biological method is an environmentally friendly and effective method for treating waste gas. However, for complex waste gas components, the conventional biological treatment effect is limited. The degradation effect of the waste gas is relatively low, and it is difficult to achieve the standard discharge of the gas. The present invention mainly aims at the composition characteristics of the gas in the petroleum and petrochemical industry, and realizes the standard discharge requirements of the gas. Degradation of functional bacteria, to achieve effective gas degradation and emission standards

Method used

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  • A kind of benzene-degrading bacteria for controlling air pollution and its preparation method and application
  • A kind of benzene-degrading bacteria for controlling air pollution and its preparation method and application
  • A kind of benzene-degrading bacteria for controlling air pollution and its preparation method and application

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Embodiment 1

[0029] The acquisition of embodiment 1 benzene degrading bacteria

[0030] The benzene-degrading functional bacteria provided by the present invention are obtained by adding benzene series to the sewage in the grease trap of an oil refinery for domestication and separation, wherein the main components in the sewage are hydrocarbon wastewater and chemical industry wastewater produced in the petroleum refining process. Production wastewater, specifically:

[0031] 1. Domestication and isolation of strains

[0032] Put the benzene-degrading functional bacteria into the sewage of the grease trap of the oil refinery according to a certain proportion, and add a certain amount of 0.1% to 0.5% (V / V) benzene to the water, and carry out 18-24h cultivation. After the cultivation, take the The culture solution is transferred to the sewage of the next grease trap according to the ratio of 5%, and 0.1% to 0.5% (V / V) of benzene is added thereto, and the cultivation is continued, and then tr...

Embodiment 2

[0045] Embodiment 2 industrialization prepares the method for bacterial agent

[0046] 1. Inoculate the slant of the strain into a 5L shake flask (with 1.5L medium inside) for production strain preparation. The medium formula of the shake flask: 10g / L peptone, 5g / L yeast extract, 10g / L NaCl , the rest is water, the culture temperature of the shaker flask is 33°C, the rotation speed of the shaker is 120rpm, and the culture time is 16h.

[0047] 2. The liquid medium formula of the seed tank is: peptone: 5g / L, yeast extract: 2g / L KH 2 PO 4 : 1g / L, NH 4 Cl: 5g / L, NaNO 3 : 3g / L, compound trace elements: 5ml / L, compound trace elements, compound trace elements are composed of: ZnSO 4 ·7H 2 O: 0.29g / L; CaCl 2 ·6H 2 O: 0.28g / L; CuSO 4 ·5H 2 O: 0.25g / L; MnSO 4 ·H 2 O: 0.17g / L.. The seed liquid is transferred to the sterilized fermented seed tank according to the volume ratio of 0.5%, and the pH value of the fermentation medium is adjusted to 6.5. MPa; ventilation rate is 2m ...

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Abstract

The invention discloses a benzene-degrading bacterium for controlling air pollution, its preparation method and application, and relates to the field of air pollution control. The date is July 11, 2017, the deposit number is CGMCC No.14406, and the deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. It can be obtained by acclimation and separation by adding benzene series. By using the benzene-degrading bacteria of the invention, the removal rate of benzene series can reach more than 90%, and the effective degradation and up-to-standard discharge of gas can be completely realized. Not only the gas treatment effect is remarkable, but also the cost is low, and no secondary pollution will be generated.

Description

technical field [0001] The invention relates to the field of atmospheric control, in particular to a bacterial strain for the control of atmospheric pollution containing benzene series, preparation of the bacterial strain and application thereof. Background technique [0002] Volatile organic compounds (VOCs for short) are a class of common pollutants, which cause great harm to the environment, the growth of animals and plants, and human health. Especially the benzene series gas in the waste gas is more toxic. The biological method is an environmentally friendly and effective method for treating waste gas. However, for complex waste gas components, the conventional biological treatment effect is limited. The degradation effect of the waste gas is relatively low, and it is difficult to achieve the standard discharge of the gas. The present invention mainly aims at the composition characteristics of the gas in the petroleum and petrochemical industry, and realizes the standard...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02B01D53/84B01D53/72C12R1/07
CPCB01D53/72B01D53/84B01D2251/95B01D2257/7027B01D2257/708C12N1/02C12N1/20C12N1/205C12R2001/07Y02A50/20
Inventor 隋志强金艳方张烨刘守成杜发洋宼焕起
Owner BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV