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Extraction process of total flavones in fructus sophorae and application thereof in treatment of hyperlipidemia

An extraction process, the technology of Sophora flavonoids, which is applied in the direction of medical preparations containing active ingredients, plant raw materials, metabolic diseases, etc., can solve the problems of low extraction rate and large amount of enzyme, and achieve high dissolution rate, extraction rate and purity Improve the effect of good drug value

Inactive Publication Date: 2018-09-11
王洲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many techniques for extracting Sophora flavonoids, most of which mainly use chemical reagents for extraction, while conventional extraction methods have unsafe factors such as organic solvent residues, which pose a potential threat to human health. Improvements have been made, such as "Process optimization of enzymatic extraction of total flavonoids in Sophora japonicus, Food Technology Science 2012" used enzymatic method to extract flavonoids from Sophora japonicus, which has the advantages of non-toxicity and mild reaction conditions, but this method has the advantages of enzyme dosage Larger, lower extraction rate and other defects, it needs to be further improved

Method used

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  • Extraction process of total flavones in fructus sophorae and application thereof in treatment of hyperlipidemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The extraction process of sophocarp flavonoids, it comprises the following steps:

[0028] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying.

[0029] Specifically include:

[0030] Take Sophora japonicus, pulverize, and pass through a 100-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 3MPa, hold the pressure for 30s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 5 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 20,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction temperature to 35°C and the reaction ti...

Embodiment 2

[0032] The extraction process of sophocarp flavonoids, it comprises the following steps:

[0033] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying.

[0034] Specifically include:

[0035] Take Sophora japonicus, pulverize, pass through a 200-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 2MPa, hold the pressure for 40s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 4 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 10,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction temperature to 35°C and the reaction time t...

Embodiment 3

[0049] Determination of total flavonoids: Taking Huaijiao in southern Shandong as an example, the experiment of the present invention uses 1000g Huaijiao, and the content of total flavonoids in Huaijiao powder measured by HPLC method is 19.7%, and the experimental groups are respectively selected from Example 1 and Comparative Example 1- 4. Obtain the weight of total flavonoids extract by weighing, measure the total flavonoids content in the extract by HPLC method, calculate the yield of total flavones, calculation formula: yield=((quality × content of extract) / (sophora japonicus quality × Content)) × 100%. The specific results are shown in Table 1:

[0050] group

[0051] As shown in the above Table 1, the yield and purity of Example 1 group are higher than those of Comparative Examples 1-4, which adopts a variety of process technologies, high voltage, pulse electric field, high-speed mechanical shearing, enzymatic method and microwave alcohol extraction method Or...

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Abstract

The invention belongs to the technical field of plant extraction, and discloses an extraction process of flavones in fructus sophorae, which includes steps of: crushing, high-pressure treatment, electric field treatment, shearing, enzyme reaction, microwave alcohol extraction, evaporation, drying, adsorption, elution, evaporation and drying. The process reduces use of chemical reagents, is high insafety and is high in extraction rate and purity of the total flavones.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an extraction process of total flavonoids from sophora japonicus and its application in treating hyperlipidemia. Background technique [0002] Sophora japonica L. is the fruit of Sophora japonica L., a leguminous plant. It is a commonly used traditional Chinese medicinal material recorded in the Pharmacopoeia. The medicinal Sophora japonica L. should usually be harvested in winter. Ripe pods. The dried Sophora japonicus pods picked after the winter solstice are cylindrical, sometimes curved, and the seeds are constricted into beads. After the foreskin, they are brown or yellowish brown, and the seeds are brownish black; Chinese medicine theory believes that Sophora japonicus is cold in nature and has a strong taste. Bitter, returns to the liver and large intestine meridian, has the functions of clearing away heat and purging fire, cooling blood and stopping b...

Claims

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Application Information

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IPC IPC(8): A61K36/489A61P3/06
CPCA61K36/489A61K2236/19A61K2236/333A61K2236/51A61K2236/55A61P3/06
Inventor 王洲孙波王芳
Owner 王洲
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