A method based on reactive oxygen species to regulate the fermentation of Mortierella alpina to produce arachidonic acid oil
A technology of Mortierella alpine and arachidonic acid, which is applied in the field of microbial fermentation, can solve the problems that products and biomass cannot be accumulated at the same time, reduce the total energy consumption of stirring, and enhance the production intensity, so as to reduce the total energy consumption of stirring and shorten the Fermentation cycle, the effect of improving production intensity
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Embodiment 1
[0029] Example 1. Based on active oxygen regulation and control of 7.5 L tank Mortierella alpina fermentation to produce arachidonic acid oil
[0030] 1. Activation of strains and preparation of seed solution: Mortierella alpina strain R807 (CCTCC M 2012118) was selected as the starting strain, and the preserved strains were inserted into PDA slant medium, and placed in an incubator at 25°C Cultivate for 10 days, transfer to a 500mL grooved bottle containing 100mL seed medium and culture in a constant temperature shaker, the inoculum size is 10% (v / v, 10mL), the culture conditions are 125rpm, the temperature is 25°C, and the culture time is 1 -2 days.
[0031]2. Inoculate the seed liquid obtained in step 1 into a 7.5L tank (New Brunswick Scientific, USA), the liquid volume is 5L, the inoculum volume is 10% (v / v, 500mL), and the culture temperature is controlled at 25°C. During the 0-60 hour period of fermentation, it is a relatively high active oxygen period. At the beginnin...
Embodiment 2
[0033] Example 2. Arachidonic acid oil produced by fermentation of Mortierella alpina in a 25 m³ tank based on active oxygen regulation
[0034] 1. Activation of strains and preparation of seed solution: Mortierella alpina strain R807 (CCTCC M 2012118) was selected as the starting strain, and the preserved strains were inserted into PDA slant medium, and placed in an incubator at 25°C Cultured for 10 days, transferred to a 500 mL grooved bottle containing 100 mL seed medium and cultured in a constant temperature shaker, the inoculum volume was 10% (v / v, 10 mL), the culture conditions were 125 rpm, the temperature was 25°C, The cultivation time is 2 days, and the primary seed liquid is prepared. Then, according to the inoculum volume of 10% (v / v), the primary seed solution was inserted into a 150 L seed tank, the cultivation conditions were 175 rpm, the temperature was 25°C, and the cultivation time was 1 day to prepare the secondary seed solution. Next, all the prepared secon...
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