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Application of trichoderma pseudokoningii exopolysaccharide as medicine for treating gastric cancer

A technology of Trichoderma cells and polysaccharides, applied in the field of medicine, can solve the problems of unreported influence on the growth of human gastric cancer cells, and achieve the effects of inhibiting tumor growth, reducing tumor weight, and increasing the concentration of reactive oxygen species.

Inactive Publication Date: 2015-02-25
SHAANXI JAINUO BIOTECH ENTERPRISE GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the functional research on the exopolysaccharide of Trichoderma pseudokonii has become a hotspot in the research of Trichoderma pseudokonii, but the research on the effect of T. pseudokonii exopolysaccharide on the growth of human gastric cancer cells has not been reported yet.

Method used

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  • Application of trichoderma pseudokoningii exopolysaccharide as medicine for treating gastric cancer
  • Application of trichoderma pseudokoningii exopolysaccharide as medicine for treating gastric cancer
  • Application of trichoderma pseudokoningii exopolysaccharide as medicine for treating gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Effects of Trichoderma pseudoconii exopolysaccharide administered in vitro on the proliferation of human gastric cancer cells BGC-823, MKN-45, MGC-803

[0041] SRB is a protein-binding dye that can combine with the basic amino acids of cellular proteins fixed by trichloroacetic acid to present a bright pink color, and its color change is proportional to the protein in living cells. ELISA The absorbance is measured by the detector to obtain an accurate value of cell protein content, which can be used to calculate the number of cells and reflect the proliferation activity of cells.

[0042] experimental method

[0043] Human gastric cancer cells BGC-823, MKN-45, and MGC-803 in the logarithmic growth phase were taken to make a density of 6×10 4 cells / ml cell suspension, seeded in 96-well plate, 150 μL / well. After culturing for 24 h, add 50 μL of exopolysaccharide solution of Trichoderma pseudoconii in different concentrations to each well (final concentration is 0.2, 0.4...

Embodiment 2

[0047] Effects of exopolysaccharide from Trichoderma pseudoconii in vitro on the chromatin morphology of human gastric cancer cell BGC-823

[0048] When cell apoptosis occurs, the permeability of the cell membrane changes, and the chromatin in the nucleus condenses and produces apoptotic bodies. Hoechst33258 dye can penetrate the cell membrane and bind to DNA, showing blue fluorescence. The nucleus or cytoplasm can be seen under a fluorescent microscope Densely dyed and dense granular block-like fluorescence appeared inside, and the morphological changes of cell nuclei were visually observed.

[0049] experimental method

[0050] Place sterile coverslips in a six-well plate, seed human gastric cancer cell BGC-823 and incubate for 24 hours. The next day, different concentrations of Trichoderma pseudoconii exopolysaccharide solutions were added to stimulate the cells for 48 hours, the culture medium was removed, 0.5ml of fixative solution (4% paraformaldehyde) was added, and th...

Embodiment 3

[0053] Effects of exopolysaccharide from Trichoderma pseudoconii in vitro on the DNA of human gastric cancer cell BGC-823

[0054] A notable feature of apoptosis is the DNA degradation of cell chromosomes, resulting in 180-200 bp double DNA fragments, which are shown in agarose gel electrophoresis as a specific ladder ladder pattern, while necrosis presents a diffuse continuous pattern .

[0055] experimental method

[0056] Human gastric cancer cells BGC-823 in the logarithmic growth phase were planted in a six-well plate and incubated for 24 hours. The next day, different concentrations of Trichoderma pseudoconii exopolysaccharide solutions were added, and after 48 hours of treatment, the cells were harvested, DNA was extracted separately, and 1% agarose gel was prepared, and the samples were loaded separately at a voltage of 80v for electrophoresis.

[0057] In vitro administration of exopolysaccharide from Trichoderma pseudoconii can induce the apoptosis of human gastric...

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Abstract

The invention relates to an application of trichoderma pseudokoningii exopolysaccharide as a medicine for treating a gastric cancer, and belongs to the technical field of a medicine and health food. Trichoderma pseudokoningii exopolysaccharide with an antitumor activity provided by the invention can obviously inhibit the growth of human gastric cancer cells BGC-823, MKN-45 and MGC-803 through in vitro administration; explosion of an reactive oxygen in the BGC-823 cell and decreasing of a mitochondrial membrane potential are caused, so that nucleic acid in a tumor cell is cracked, a DNA (deoxyribonucleic acid) segment is produced, a BGC-823 cell cycle is stopped at an S stage, and finally, apoptosis of cancer cells occurs, and therefore, the antitumor effect is produced. The medicine administrated in vivo can inhibit tumor growth of a BGC-823 tumor-bearing mouse, an MKN-45 tumor-bearing mouse and an MGC-803 tumor-bearing mouse, and relieves tumor weight, so that trichoderma pseudokoningii exopolysaccharide has a remarkable antitumor effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and belongs to the application of Trichoderma pseudoconii exopolysaccharide as a drug for treating gastric cancer. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. According to statistics from the World Health Organization in 2008, about 12.7 million people in the world were diagnosed with tumors, and nearly 7.6 million people died of tumors. According to reports, more than 21 million people will suffer from tumors in 2030. Thirteen million people will die from cancer. According to the data survey, the cases of death due to tumor account for about 13% of all deaths in the world. The most serious and harmful tumors in my country include gastric cancer, lung cancer, nasopharyngeal cancer, esophageal cancer, colorectal cancer, liver cancer, breast cancer, cervical cancer, leukemia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P35/00
Inventor 陈靠山林俊李萍钱文
Owner SHAANXI JAINUO BIOTECH ENTERPRISE GROUP
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