Breeding method of tomato disease-resisting homozygotes

Abstract

The invention discloses a breeding method of tomato disease-resisting homozygotes. The method comprises a tissue culture and identification step and a disease-resisting screening step, wherein tissueculture comprises the following steps: preparing a specific culture medium formula based on a conventional culture medium formula by replacing sucrose with trehalose and adding nutrient components oflycium ruthenicum juice filtrate and preparing an explant, carrying out callus induced culture on anther, carrying out callus culture, obtaining a complete plant, treating with colchicine and carryingout ploidy determination to obtain homozygote seedlings; combining a molecular marking technology to carry out disease-resisting molecular marker screening, so as to obtain disease-resisting homozygote plants. According to the method disclosed by the invention, a screening period can be remarkably shortened and the tomato disease-resisting homozygotes are rapidly obtained; moreover, the method issimple to operate and low in cost; after anther culture seedlings are transplanted, the survival rate is high and the stress resistance is strong; the method is easy to popularize, can be directly applied to production and has relatively good economical benefits and social benefits.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for cultivating tomato disease-resistant homozygotes. Background technique [0002] Tomato is an annual or perennial plant of the Solanaceae family, and it is one of the most important vegetable crops in the world. For a long time, various tomato diseases have been a huge threat to production, especially since the 1960s, with the widespread planting of tomatoes all over the country, various diseases spread rapidly and increase year by year. Breeding of disease-resistant varieties is the most effective control method with significant economic and ecological benefits. [0003] In traditional breeding, the acquisition of high-generation pure and inbred parent materials that are resistant to multiple diseases mainly uses selfing, hybridization and backcrossing technology, which usually takes 3 to 5 years to complete. The breeding cycle is long and the breeding success...

AI Technical Summary

Problems solved by technology

[0003] In traditional breeding, the acquisition of high-generation pure and inbred line parent materials that are resistant to various diseases mainly uses self-crossing, hybridization and backcrossing techniques, which usually take 3 to 5 years to complete. The breeding cycle is long and the breeding success rate is low. Therefore, There is an urgent need to establish a complete set of efficient breeding system to improve breeding efficiency and effectively serve production

Images