Breeding method of tomato disease-resisting homozygotes

A breeding method and homozygous technology, applied in the field of crop breeding, can solve problems such as low breeding success rate and long breeding cycle, and achieve the effects of increasing induction rate, increasing success rate, and improving stress resistance

Active Publication Date: 2018-09-14
SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In traditional breeding, the acquisition of high-generation pure and inbred line parent materials that are resistant to various diseases mainly uses self-crossing, hybridization and backcrossing techniques, which usually take 3 to 5

Method used

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  • Breeding method of tomato disease-resisting homozygotes
  • Breeding method of tomato disease-resisting homozygotes
  • Breeding method of tomato disease-resisting homozygotes

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Experimental program
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Effect test

Embodiment 1

[0044] Tomato anthers were taken as explants, and 6 different medium treatments were set up, each of which was repeated 3 times. The specific technical scheme is as follows:

[0045] Treatment 1: Tomato anther haploid culture is carried out according to the method of the present invention.

[0046] Yu Qingtian collected 6-8 mm long flower buds from the disease-resistant tomato mutant plants in the greenhouse of the Shouguang Vegetable Group Breeding Base, which were pre-checked by pressing, and placed at 4°C for pretreatment for 4 days. The flower buds were sterilized with 75% alcohol for 30 seconds before inoculation, then with 0.1% mercuric chloride for 10 minutes, and then rinsed 4 times with sterile water. The anthers were taken out from the flower buds under sterile conditions, and the anthers with a length of 2-5 mm (the pollen development period in them was the mononuclear border stage) were selected for anther culture. The anthers were inoculated in MS basic medium, s...

Embodiment 2

[0073] Practical application: Tomato anther haploid culture is carried out according to the method of the present invention.

[0074] Yu Qingtian collected 6-8 mm long flower buds from disease-resistant mutant tomato plants in the greenhouse of Shouguang Vegetable Group Breeding Base, and placed them at 4°C for 4 days of pretreatment. The flower buds were sterilized with 75% alcohol for 30 seconds before inoculation, then with 0.1% mercuric chloride for 10 minutes, and then rinsed 4 times with sterile water. The anthers were taken out from the flower buds under sterile conditions, and the anthers with a length of 2-5 mm (the pollen development period in them was the mononuclear border stage) were selected for anther culture. The anthers were inoculated in MS basic medium, KT 0.5mg / L, 2,4-D 1mg / L, trehalose 30g / L, black fruit Lycium barbarum juice filtrate 20g / L and agar 8g / L, pH 5.8 culture base for callus induction culture. Culture conditions: 80% relative humidity, 24-26°C...

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Abstract

The invention discloses a breeding method of tomato disease-resisting homozygotes. The method comprises a tissue culture and identification step and a disease-resisting screening step, wherein tissueculture comprises the following steps: preparing a specific culture medium formula based on a conventional culture medium formula by replacing sucrose with trehalose and adding nutrient components oflycium ruthenicum juice filtrate and preparing an explant, carrying out callus induced culture on anther, carrying out callus culture, obtaining a complete plant, treating with colchicine and carryingout ploidy determination to obtain homozygote seedlings; combining a molecular marking technology to carry out disease-resisting molecular marker screening, so as to obtain disease-resisting homozygote plants. According to the method disclosed by the invention, a screening period can be remarkably shortened and the tomato disease-resisting homozygotes are rapidly obtained; moreover, the method issimple to operate and low in cost; after anther culture seedlings are transplanted, the survival rate is high and the stress resistance is strong; the method is easy to popularize, can be directly applied to production and has relatively good economical benefits and social benefits.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for cultivating tomato disease-resistant homozygotes. Background technique [0002] Tomato is an annual or perennial plant of the Solanaceae family, and it is one of the most important vegetable crops in the world. For a long time, various tomato diseases have been a huge threat to production, especially since the 1960s, with the widespread planting of tomatoes all over the country, various diseases spread rapidly and increase year by year. Breeding of disease-resistant varieties is the most effective control method with significant economic and ecological benefits. [0003] In traditional breeding, the acquisition of high-generation pure and inbred parent materials that are resistant to multiple diseases mainly uses selfing, hybridization and backcrossing technology, which usually takes 3 to 5 years to complete. The breeding cycle is long and the breeding success...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程琳武玉芬国家进潘子龙胡永军于彩云许勇王茂昌李晓杰
Owner SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD
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