Dendrocalamus farinosus breeding method through in-vitro induced mutation by using ethylmethane sulfonate

Abstract

The invention discloses a Dendrocalamus farinosus breeding method through in-vitro induced mutation by using ethylmethane sulfonate. The invention provides a method for obtaining mutant plants of Dendrocalamus farinosus. The method comprises the following step: carrying out induced mutation on calli derived from the Dendrocalamus farinosus by using ethylmethane sulfonate. The method for obtainingthe mutant plants of the Dendrocalamus farinosus further comprises the following step: culturing the induced-mutated calli to obtain regenerated plants. The method for obtaining the mutant plants of the Dendrocalamus farinosus further comprises the following step: screening the mutant plants from the regenerated plants. According to the method provided by the invention, an induced mutation technology and in-vitro culture are combined, and the calli of the Dendrocalamus farinosus are subjected to in-vitro induced mutation by using ethylmethane sulfonate, so that a large quantity of homogeneousinduced-mutated colony can be obtained. According to the method, the mutation frequency and breeding efficiency are increased extremely, the improvement on induced mutation breeding of the Dendrocalamus farinosus is facilitated, the industrialized development of the Dendrocalamus farinosus is promoted, and the method has a great application and popularization value.

Description

technical field

[0001] The invention belongs to the field of plant mutation breeding, and in particular relates to a breeding method for using ethyl methanesulfonate to induce mutagenesis of Ciba japonica in vitro. Background technique

[0002] The pulp and paper industry currently has the problem of relatively low recycling rate of waste paper. Bamboo, as a wide range of plant natural resources, has the characteristics of wide distribution, strong adaptability, fast growth, early maturity, and high economic value. After a successful afforestation, it can be felled every year after 3-5 years, and lasts for decades. Even hundreds of years. The cellulose content of bamboo is 40%-60%, its excellent pulping performance can be compared with some wood fibers, and the fiber shape quality of bamboo pulp is between straw pulp and wood pulp, closer to wood pulp. Prompting it to become an excellent non-wood papermaking raw material. Therefore, cultivating bamboo varieties with large...

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