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Microfluidic chip based on micro liquid drops and detection system

A microfluidic chip and microdroplet technology, applied in the field of clinical in vitro detection and biotechnology, can solve the problems of low repeatability, high cost, and large sample demand.

Active Publication Date: 2018-09-14
上海艾瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these systems are still unsatisfactory, and there are different shortcomings, for example: some microfluidic chips can perform high-throughput detection, but have complex structures, high costs, and low sensitivity; some use heterogeneous reactions , so the sample requirement is large and the repeatability is low; and most of the above-mentioned microfluidic chips are disposable, which is not conducive to popularization

Method used

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  • Microfluidic chip based on micro liquid drops and detection system
  • Microfluidic chip based on micro liquid drops and detection system
  • Microfluidic chip based on micro liquid drops and detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0221] Detection of BNP in human blood samples by chemiluminescent method

[0222] In this example, using figure 1 The microfluidic chip shown uses chemiluminescent method to detect BNP in human blood samples.

[0223] 1. Experimental method

[0224] 1. Carry out the pretreatment of blood samples and reagents, that is, take human blood samples A and B, and add them into EDTA-anticoagulant blood collection tubes to prevent coagulation.

[0225] 2. The treatment of BNP reagent: the BNP antibody is coated with magnetic beads, and the BNP antibody is labeled with acridine.

[0226] 3. Preparation of chemiluminescent substrate: equal volume of 0.1M HNO 3 , 0.1%H 2 o 2 Luminescence excitation solution A is mixed with 0.25M NaOH, 2% Tween-20 luminescence excitation solution B to form a chemiluminescence excitation substance.

[0227] 4. Sample injection: in figure 1 Add sample A to the dispersed phase inlet 6 shown, in figure 1 Continuous phase mineral oil is added to the con...

Embodiment 2

[0244] Detection of B-type natriuretic peptide (BNP) by double-antibody sandwich method

[0245] In this example, using figure 1 The microfluidic chip shown uses the double-antibody sandwich method to detect BNP in human blood samples.

[0246] 1. Experimental method

[0247] 1. same as embodiment 1, first carry out the pretreatment of blood sample and reagent: get human blood sample A in EDTA anticoagulant tube, to prevent coagulation.

[0248] 2. Treat the two antibodies of the double-antibody sandwich method: fluorescently labeled antibody 1 and magnetic bead-coupled antibody 2.

[0249] 3. Sample injection: in figure 1 Add sample A to the dispersed phase inlet 6 shown, in figure 1 Add the continuous phase mineral oil in the continuous phase inlet 1 shown, at this moment the control valve 5 is closed, and the control valve 7 is opened. figure 1 The shown sample outlet 28 and waste liquid port 4 give negative pressure as a power source, and the design of the sample inle...

Embodiment 3

[0264] Construction of standard curve for detection of B-type natriuretic peptide (BNP) by double-antibody sandwich method

[0265] In this example, for the detection of B-type natriuretic peptide (BNP) by the double-antibody sandwich method based on the immune response, the results in different extraction processes were compared, and a standard curve was prepared for it. In addition, based on the test results, the cleaning effects were compared.

[0266] The method is as follows: first prepare a series of concentrations of BNP calibrator; using the same calibrator, the number of micro-droplets cleaned during (a) extraction is twice that of the sample droplets; and (b) the number of micro-droplets cleaned during extraction is In the case of twice the sample droplet, the determination is carried out separately, the results of different extraction processes are compared, and a standard curve is made.

[0267] The comparison results are as Image 6 , Figure 7 , and shown in T...

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Abstract

The invention provides a microfluid control system used for biosample detection and based on micro liquid drops, and particularly provides a microfluidic chip which comprises a microfluid circulatingpassage, a reaction micro liquid drop generation module, a cleaning micro liquid drop generation module, a cutting micro liquid drop generation module, a capturing-cleaning-cutting module, a signal reading and detection module and a liquid drop queue control module and can selectively comprises an incubation control module and a waste liquid collection module.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and clinical in vitro detection, and in particular relates to a microfluidic chip and a detection system based on microdroplets. Background technique [0002] In Vitro Diagnosis (IVD) refers to products and services that obtain clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.) outside the human body, and then determine diseases or body functions. There are many traditional methods for biological detection and diagnosis in the prior art, such as biochemical detection methods, molecular detection and analysis methods, western blotting, chemiluminescence immunoassay, enzyme-linked immunoassay (ELISA), etc. However, these detection methods require a relatively long analysis time, the liquid processing process is cumbersome, the throughput is relatively small, and the accuracy is insufficient. [0003] Recently, the microfluidic system, as a platform c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/53B01L3/00
CPCB01L3/5027G01N21/76G01N33/53
Inventor 王陈成罗贞王茜马云林
Owner 上海艾瑞德生物科技有限公司