Method for ultrafiltration removal of proteins from yellow serofluid by using enzyme membrane reactor

Abstract

The invention discloses a method for ultrafiltration removal of proteins from yellow serofluid by using an enzyme membrane reactor, and belongs to the technical field of food biochemistry. The methodcomprises the steps: A, preparation of a raw material: after collecting the yellow serofluid, carrying out coarse filtration, and adjusting the pH; B, preparation of an ultrafiltration enzyme membrane: respectively placing a membrane into a mixed liquid I, a mixed liquid II and a mixed liquid III, soaking, and then cleaning, to obtain the ultrafiltration enzyme membrane; and C, enzyme membrane reactor treatment: placing the ultrafiltration enzyme membrane in a reactor, constructing an enzyme membrane reactor, then adding a pretreatment liquid into the enzyme membrane reactor, and carrying outultrafiltration removal treatment. The efficiency and quality of ultrafiltration recovery of the proteins in the tofu yellow serofluid are improved by depending on the enzyme membrane reactor, the enzyme membrane circulation utilization ratio is effectively improved, at the same time, the waste of resources is reduced, the discharge capacity is reduced, and environmental friendliness is guaranteed.

Description

technical field [0001] The invention relates to a method for filtering and removing protein in yellow pulp water, in particular to a method for ultrafiltering protein in yellow pulp water by using an enzyme membrane reactor, and belongs to the technical field of food biochemistry. Background technique [0002] my country is a major producer of traditional soy products. During tofu processing, about 35-50% of the yellow pulp is leached out during the solidification of hot soybean milk and the pressing of billets, and most of the yellow pulp is discharged into the environment after simple treatment. Among them, the displacement is huge. According to statistics: processing 1kg of soybeans can produce 2-5kg of yellow slurry water, which not only causes environmental pollution, but also leads to waste of resources. Yellow pulp water contains a variety of active ingredients, among which the most important macromolecular active ingredient is soybean whey protein, whose content is as...

AI Technical Summary

Problems solved by technology

[0003] Ultrafiltration technology is considered to be one of the effective means to recover and remove yellow pulp water protein, but the reason that affects the widespread use of ultrafiltration technology is: the molecular size of various globulins in yellow pulp water is different, and some of the small size Globulin is easy to block the pores of the ultrafiltration membrane, and it is easy to cause membrane fouling after long-term use, resulting in a sharp decrease in membrane flux, and the ultrafiltration membrane needs to be cleaned regularly, which directly affects the working efficiency of the ultrafiltration

Patent document CN101473887A (a method for membrane separation and recovery of protein in tofu yellow pulp water, 2009.07.08) discloses that before ultrafiltration, the tofu wastewater is pretreated with enzymes, so that small molecular soybean whey proteins can be polymerized to form polysaccharides before ultrafiltration. Polymers can expand their molecular weight, thereby improving their protein recovery and membrane flux, but this patent also has some shortcomings: 1) The pretreated enzyme is only effective for a single batch of reactions and cannot be recycled; 2) After the enzyme reaction is completed, in order to prevent the enzyme from over-reacting, it is necessary to kill the enzyme at high temperature, which increases the treatment process and cannot be recycled.

[0004] Patent document CN101724555A (a whey protein hypotensive peptide prepared by continuous enzyme membrane reactor and its special device, 2010.06.09), patent document CN103233054A (method for continuous preparation of silkworm chrysalis protein antioxidant peptide by enzyme membrane reactor, 2013.08.07), patent document CN102212600A (a method for preparing oat antioxidant peptides using an enzyme membrane reactor, October 12, 2011) and patent document CN102559812A (method for preparing maltose syrup by continuous saccharification in an enzyme membrane reactor, 2012.07. 11) Both disclose that raw materials and corresponding specific enzymes are added to the enzyme membrane reactor to react to obtain the target product, but this is not conducive to the recycling of enzymes and filter membranes; at the same time, the enzymes in the above membrane enzyme reactors are all It is a decomposing enzyme, which decomposes the substrate (such as: protein, starch, etc.) while being expelled

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