Method for preparing biofuel cell by using trehalose produced by yeast

A biofuel cell and trehalose technology, applied in the fields of chemistry and biology, can solve single problems, achieve the effects of avoiding environmental pollution, strong growth ability, and improving utilization rate

Active Publication Date: 2018-09-18
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Pseudomonas is used as an anode strain, and glucose and other substances are used as organic substrates to form a fuel cell (public number: CN102255096A), and brewer’s yeast and culture medium are also used as anodes to metabolize anolyte glucose to form a fuel cell (public number: CN102255096A). : CN1588683A), but the anode bacteria used in these are all single strains, and need to add exogenous organic matter as anodic reaction

Method used

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  • Method for preparing biofuel cell by using trehalose produced by yeast
  • Method for preparing biofuel cell by using trehalose produced by yeast
  • Method for preparing biofuel cell by using trehalose produced by yeast

Examples

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Effect test

Embodiment 1

[0037] (1) Weigh 6.1mg of 222 u / mg glucose oxidase solid into a 150mL Erlenmeyer flask, add distilled water and stir to prepare 50mL of 27 u / mL glucose oxidase solution, place a blank carbon felt electrode (surface area 1.5cm× 1.5cm) into the solution, soak at 200r / min 37°C for 12 hours, take it out, and refrigerate at -4°C for later use;

[0038] (2) Inoculate 2 mL of yeast cryopreservation liquid into a 250 mL Erlenmeyer flask filled with 50 mL of YPD culture liquid, shake the flask at 200 r / min 28-30°C for 24 h, and obtain yeast fermentation liquid for later use;

[0039] After about 12 h of yeast fermentation, inoculate 2 mL of E. coli frozen stock solution into a 250 mL Erlenmeyer flask filled with 50 mL of LB culture liquid, and shake the flask at 200 r / min at 37°C for 12 h to obtain E. coli fermentation liquid. ;

[0040] Centrifuge the yeast fermentation broth and E. coli fermentation broth at 2800rpm for 10 minutes respectively, wash twice with PBS, and then centrifu...

Embodiment 2

[0045] (1) Weigh 6.1mg of 222 u / mg glucose oxidase solid into a 150mL Erlenmeyer flask, add distilled water and stir to prepare 50mL of 27 u / mL glucose oxidase solution, place a blank carbon felt electrode (surface area 1.5cm× 1.5cm) into the solution, soak at 200r / min 37°C for 12 hours, take it out, and refrigerate at -4°C for later use;

[0046] (2) Inoculate 2mL of yeast cryopreservation liquid into a 250mL Erlenmeyer flask filled with 50mL of YPD culture liquid, shake the flask at 200r / min 28-30°C for 24 hours, and obtain a yeast fermentation liquid for later use;

[0047] After about 12 h of yeast fermentation, inoculate 2 mL of E. coli frozen stock solution into a 250 mL Erlenmeyer flask filled with 50 mL of LB culture liquid, and shake the flask at 200 r / min at 37°C for 12 h to obtain E. coli fermentation liquid. ;

[0048] Centrifuge the yeast fermentation broth and E. coli fermentation broth at 2800rpm for 10 minutes respectively, wash twice with PBS, and then centri...

Embodiment 3

[0053] (1) Weigh 6.1mg of 222 u / mg glucose oxidase solid into a 150mL Erlenmeyer flask, add distilled water and stir to prepare 50mL of 27 u / mL glucose oxidase solution, place a blank carbon felt electrode (surface area 1.5cm× 1.5cm) into the solution, soaked at 200r / min 37°C for 12 hours, took it out, and refrigerated at -4°C for later use.

[0054] (2) Inoculate 2mL of yeast cryopreservation liquid into a 250mL Erlenmeyer flask filled with 50mL of YPD culture liquid, shake the flask at 200r / min28-30℃ for 24 hours to obtain yeast fermentation liquid, and set aside;

[0055] After about 12 h of yeast fermentation, inoculate 2 mL of E. coli frozen stock solution into a 250 mL Erlenmeyer flask filled with 50 mL of LB culture liquid, and shake the flask at 200 r / min at 37°C for 12 h to obtain E. coli fermentation liquid. ;

[0056] Centrifuge the yeast fermentation broth and E. coli fermentation broth at 2800rpm for 10 minutes respectively, wash twice with PBS, and then centrifu...

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Abstract

The present invention relates to a method of preparing a biofuel cell by using trehalose produced by yeast. Saccharomycetes, Escherichia coli and glucose oxidase are simultaneously immobilized on a carbon felt electrode as an anode, a blank carbon felt electrode is used as a cathode, a saccharomycetes fermentation culture liquid is used as an anolyte, a hydrogen peroxide solution is used as a catholyte, and a proton exchange membrane is disposed between a cathode chamber and an anode chamber to form the biofuel cell. A multiple-enzyme conjugation reaction is designed, has the reaction characteristics of being high-efficiency and precise, and does not use a strong acid, a strong alkali and an organic solvent, environmental pollution can be avoided, and the reaction is green and environmentally-friendly.

Description

technical field [0001] The invention relates to the fields of chemistry and biology, in particular to a method for preparing biofuel cells by using yeast to produce trehalose. Background technique [0002] A fuel cell is a device that directly converts the chemical energy of fuel and oxidant into electrical energy through an electrochemical reaction. It is widely used in military, national defense and civilian power, automobiles, communications and other fields, and has extremely broad application prospects. The membrane electrode assembly is the core unit of the fuel cell. It is composed of a proton-conducting membrane and electrodes (cathode and anode) on both sides of the membrane. The fuel is supplied to the anode, and the oxidant is supplied to the cathode, thereby generating redox reaction. [0003] A biofuel cell is a device that uses the principle of a fuel cell to convert chemical energy in organic matter into electrical energy by using organic matter such as gluco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M8/16H01M4/90H01M8/00
CPCH01M4/90H01M8/00H01M8/16Y02E60/50
Inventor 朱丽英高姗徐阳雪江凌黄和
Owner NANJING UNIV OF TECH
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