Recombinant adiponectin antigen, antibody and adiponectin nano-latex enhanced immunoturbidimetric assay kit

A technology of recombinant lipid and adiponectin, applied in the direction of anti-hormone immunoglobulin, anti-animal/human immunoglobulin, hormone peptide, etc. Significance, not explaining the normal level and reference range of adiponectin measured, limiting the clinical promotion and application of adiponectin, etc., to achieve the effect of good prediction effect, high accuracy and simple steps
CN108570104BActive Publication Date: 2021-11-12英诺生物技术(深圳)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
英诺生物技术(深圳)有限公司
Publication Date
2021-11-12

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Abstract

The invention discloses a recombinant adiponectin antigen, the preparation of which comprises the following steps: a. culturing a cell line highly expressing recombinant human full-length adiponectin protein in a high-sugar DMEM medium, and the culture condition is 0.25-0.75L / min oxygen flow rate, 3-6% carbon dioxide volume, 5-10% bovine serum volume, 100-200rpm speed, so that the cell line reaches 1-3×10 7 b. Replace the medium with serum-free medium and add 0.5% vitamin C to maintain the cell line at 1-3×10 7 The number of cells continued to express adiponectin for 4 to 10 days. The invention also discloses adiponectin antibody, adiponectin antibody nano latex particles and a kit containing the antigen and antibody nano latex particles. The kit adopts the nano latex-enhanced immune turbidimetric method to detect adiponectin with high accuracy, true and reliable data, and good prediction effect on type 2 diabetes and prediabetes.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a recombinant adiponectin antigen, antibody and adiponectin nano-latex enhanced immune turbidimetry kit and application thereof. Background technique

[0002] With the progress of society, changes in the age structure of the population and changes in living habits, the incidence of diabetes has increased rapidly and has become a global epidemic (American Diabetes Association, Diabetes care36Suppl 1:S67-74(2013)). According to the International Diabetes Federation, there are currently more than 380 million diabetic patients worldwide, and 46% of them are not discovered until serious diabetic complications occur. According to statistics, the global annual expenditure on diabetes health care exceeds 548 billion US dollars (International Diabetes Federation, IDF Diabetes Atlas, 6th edition / 2013 update (2013)). According to recent epidemiological surveys, the prevalence of diabetes in my...

Claims

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