Small molecular nutrient for effectively improving calcium absorption rate of elderly population and preparation method thereof

A small molecule and absorption rate technology, applied in the field of small molecule nutrients and their preparation, can solve problems such as the quality requirements of operators, and achieve the effects of efficient preparation method, improved calcium absorption rate, and low cost

Inactive Publication Date: 2018-09-28
江瀚生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction conditions of the chemical modification of the above method require high temperature and the addition of irritating hydrogen halide gas, a large amount of chemical raw materials need to be used, and there are high quality requirements for operators.
[0006] Therefore, a product with high efficiency, low cost and high equol content has not been obtained at present to meet the needs of the market for improving the calcium absorption rate of the elderly population

Method used

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  • Small molecular nutrient for effectively improving calcium absorption rate of elderly population and preparation method thereof
  • Small molecular nutrient for effectively improving calcium absorption rate of elderly population and preparation method thereof

Examples

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Effect test

Embodiment 1

[0034] A method for preparing a small molecule nutrient that effectively improves the calcium absorption rate of the elderly population, comprising the following steps:

[0035] Step 1, sending the red clover directly into the crushing equipment, kneading and crushing the broken grass to a length of 1 to 2 cm, and obtaining the red clover fermentation raw material;

[0036] Step 2, first cultivating Saccharomyces cerevisiae, Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis subsp. lactis as seed liquid:

[0037] Transfer the above Saccharomyces cerevisiae into the Erlenmeyer flask of potato dextrose liquid medium, shake and culture at 28°C and 220rpm for 48 hours, take out the medium after the medium is cloudy, and then put it in a refrigerator at 2-4°C for storage until use;

[0038] Transfer the above-mentioned Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis lactic acid subspecies into conical flasks of MRS liquid medium, culture at...

Embodiment 2

[0043] A method for preparing a small molecule nutrient that effectively improves the calcium absorption rate of the elderly population, comprising the following steps:

[0044] Step 1, sending the red clover directly into the crushing equipment, kneading and crushing the broken grass to a length of 1 to 2 cm, and obtaining the red clover fermentation raw material;

[0045] Step 2, first cultivating Saccharomyces cerevisiae, Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis subsp. lactis as seed liquid:

[0046] Transfer the above-mentioned Saccharomyces cerevisiae into the conical flask of potato dextrose liquid medium, shake and culture at 30°C and 200rpm for 48 hours, take out the medium after the medium is cloudy, and then put it in a refrigerator at 2-4°C for storage until use;

[0047] Transfer the above-mentioned Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis lactic acid subspecies into conical flasks of MRS liquid medium, cultu...

Embodiment 3

[0052] Accurately weigh about 0.5 g of the fermented red clover isoflavone powder prepared in Example 2, put it in a 50 mL bottle with a stopper, add 20 mL of 70% ethanol precisely, weigh it, and ultrasonically treat it for 30 minutes. Determine the weight, add 70% ethanol to make up the lost weight, shake well, filter, and take the filtrate as the test solution.

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Abstract

The present invention provides a preparation method of a small molecular nutrient for effectively improving a calcium absorption rate of an elderly population. The preparation method comprises the following steps: step 1, trifolium pretense is directly sent into a crushing device for kneading and crushing into broken grass with a length of 1-2 cm to obtain a fermentation raw material; step 2, a micro-organism stock solution obtained by mixing saccharomyces cerevisiae, lactobacillus casei, lactobacillus acidophilus and lactococcus lactis subsp. lactis at a weight ratio is inoculated into the prepared fermented raw material in the step 1; and a certain amount of sugar is added for fermentation to obtain a fermentation product; and step 3, the prepared fermentation product in the step 2 is separated to obtain a liquid fermented product. The small molecular nutrient is relatively high in content of equol, highly efficient in the preparation method and low in costs, and can improve the calcium absorption rate of the elderly population.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a small-molecule nutrient that can effectively improve the calcium absorption rate of elderly people and a preparation method thereof. Background technique [0002] Red clover (Trifolium pratense L.) is a leguminous perennial herb, also known as red clover, red clover and clover. Originally produced in Europe and West Asia, it is now widely distributed all over the world. It is a temperate distribution plant and is cultivated in all parts of North and South China. Red clover contains flavonoids, proteins, amino acids, sugars and vitamins. Red clover extract is widely used in health food, among which chickpein A, chickpein B and genistein are metabolized by intestinal microorganisms to produce equol, which has phytoestrogens-like effects. It is considered to be the key substance for soybean isoflavones to play a physiological role. It can increase the level of active isoflavones in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00C12N1/20C12N1/18C12R1/865C12R1/245C12R1/23C12R1/01
CPCA23L33/00C12N1/18C12N1/20A23V2002/00A23V2400/157A23V2400/231A23V2400/125A23V2400/113A23V2200/30
Inventor 江瀚
Owner 江瀚生物科技(上海)有限公司
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