Unlock instant, AI-driven research and patent intelligence for your innovation.

Extraction method for xanthophyll from bloom-forming cyanobacteria

A technology of cyanobactin and cyanobacteria is applied in the field of resource utilization of cyanobacteria, which can solve the problems of low extraction purity, shortened extraction time, long time consumption, etc., and achieves time saving, shortened extraction time and high efficiency. Effect

Inactive Publication Date: 2018-09-28
INST OF AQUATIC LIFE ACAD SINICA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to provide a method for extracting lutein from cyanobacteria, which is easy to operate, simple to operate, simple, efficient, low-cost, and fast to extract lutein from cyanobacteria cells, including The two parts of extraction and purification greatly shorten the extraction time, and solve the technical problems of the existing methods such as long time consumption, low efficiency, low extraction purity and high cost. The production of lutein provides another source of raw materials, and at the same time provides an important application direction for the resource utilization of algae biomass

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method for xanthophyll from bloom-forming cyanobacteria
  • Extraction method for xanthophyll from bloom-forming cyanobacteria
  • Extraction method for xanthophyll from bloom-forming cyanobacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A method for extracting cyanobacterial lutein, the steps of which are:

[0048] 1. Selection of blooming cyanobacteria. Microcystis was selected, and the cyanobacteria bloom in China is dominated by Microcystis, and it is more typical to select Microcystis as the representative of bloom cyanobacteria.

[0049] 2. Pre-treatment of Microcystis. The Microcystis harvested from the water body is first filtered through a No. 25 phytoplankton net to remove most of the water, and then spray-dried to make algal powder for extracting Microcystis lutein.

[0050] 3. Disintegration of Microcystis cells. Accurately weigh 0.1 or 0.5 or 0.8g of algae powder and add 90% (v / v) ethanol at a ratio of 1:20, through the BRANSON-S450D ultrasonic cell disruptor, start for 3 seconds and stop for 2 seconds, 40% frequency Microcystis cells were disrupted by a 20 min disruption program.

[0051] 4. Extraction of Microcystis lutein. Hitachi CR21GⅡ high-speed refrigerated centrifuge at 10000r / ...

Embodiment 2

[0064] A method for extracting cyanobacterial lutein, the steps of which are:

[0065] 1. Selection of blooming cyanobacteria. With embodiment 1.

[0066] 2. Pre-treatment of Microcystis. With embodiment 1.

[0067] 3. Disintegration of Microcystis cells. Accurately weigh 0.1 or 0.3 or 0.6 or 0.9g of algae powder and add 90% (v / v) ethanol at a ratio of 1:10 or 1:20 or 1:30, and start 3 seconds off, 2 seconds off, 40% frequency of breaking 15 or 20 or 25 minutes program to break Microcystis cells.

[0068] 4. Extraction of Microcystis lutein. Hitachi CR21GⅡ high-speed refrigerated centrifuge at 10,000r / min for 4 or 5 or 6min, collect the supernatant, then add 5mL of 90% (v / v) ethanol to the centrifuge tube, start for 3 seconds and stop for 2 seconds, ultrasonic at 40% frequency Shake for 4 or 5 or 6 minutes and repeat the extraction 3 or 4 or 5 or 6 times until the solution becomes clear.

[0069] 5. Purification of Microcystis lutein. Add 20% (w / v) potassium hydroxide-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method for xanthophyll from bloom-forming cyanobacteria. The method comprises the following steps: A, selecting the bloom-forming cyanobacteria: taking a sample of the bloom-forming cyanobacteria, and performing detection by using a microscope; B, performing treatment on the cyanobacteria in an earlier stage: collecting the selected cyanobacteria, and performing spray drying to prepare cyanobacteria powder; C, performing cell disruption on the cyanobacteria: performing disruption by using ultrasonic waves; D, extracting the xanthophyll from the cyanobacteria: performing centrifugation by using a high-speed refrigerated centrifuge, collecting a supernatant liquid, and adding ethanol until the extraction liquid becomes clear; E, performing purification on the cyanobacteria xanthophyll: adding a potassium hydroxide-ethanol solution into the supernatant liquid in proportion, and performing saponification at temperature of 4 DEG C, adding petroleum ether, performing extraction, and washing the organic phase by using distilled water until the pH is neutral; F, performing concentration on a finished product of the xanthophyll, and performing drying: performing aeration drying on the xanthophyll solution by using a nitrogen evaporator in a fume hood to prepare the xanthophyll; and G, storing the finished product of the xanthophyll: putting the xanthophyll into a valve bag, and performing storage in the dark. The method disclosed by the invention is simple and easy to operate, has low costs, high efficiency and good product safety, and is suitable for industrialized rapid and large-scale extraction of the cyanobacteria xanthophyll.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of cyanobacteria in water blooms, specifically relates to a pigment extraction technology of cyanobacteria in water blooms, and more specifically relates to a method for extracting lutein from cyanobacteria in water blooms, which is suitable for extracting high-value components in cyanobacteria in water blooms. Background technique [0002] With the rapid development of social economy, large shallow lakes in the middle and lower reaches of the Yangtze River are showing serious eutrophication and accumulation of cyanobacteria blooms. There are many algae that can form water blooms, such as cyanobacteria, green algae, diatoms, dinoflagellates, etc. Water blooms can form below. Some of these algae can produce odorous substances, and some can produce toxins, which are extremely harmful to the water environment. Among them, cyanobacterial blooms have the widest distribution range, the lon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 王高鸿赵艳苹汪志聪李小燕李敦海
Owner INST OF AQUATIC LIFE ACAD SINICA