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Cyclic dipeptide and application of cyclic dipeptide in preparation of bactericide

A technology of cyclic dipeptide and bactericide, applied in the field of cyclic dipeptide and its application in the preparation of bactericide, to achieve the effect of easy degradation

Active Publication Date: 2018-09-28
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inhibitory effect of cyclic (L-proline-L-valine) on plant pathogenic bacteria has not been reported in domestic literature so far

Method used

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  • Cyclic dipeptide and application of cyclic dipeptide in preparation of bactericide

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Experimental program
Comparison scheme
Effect test

Embodiment 2

[0019] Embodiment 2 loop (L-proline-L-valine) inhibits pathogenic bacteria growth activity assay

[0020] The test bacteria with consistent activity were inoculated into PDA plates containing 200ppm, 100ppm, 50ppm, 25ppm, and 12.5ppm ring (L-proline-L-valine) respectively.

[0021] Three replicates were set up for each group of experiments, and three blank controls were set up.

[0022] Cultivate at 25°C until the blank control colonies cover two-thirds of the petri dish, then measure the experimental results.

[0023] Table 1 shows the results of the determination of the growth inhibitory activity of cyclic dipeptide (L-proline-L-valine) on pathogenic bacteria.

[0024] Table 1

[0025] strain

Embodiment 3

[0026] The control of embodiment 3 cucumber target spot disease

[0027] Spray once in the early stage of the disease, and 3 parallel treatment plots, each with an area of ​​20 square meters. The incidence was investigated before and 10 days after the application, and 5 points were randomly selected in each plot for sampling, and the percentage of the lesion area on each leaf of the whole plant to the leaf area was investigated, and the disease index and control effect were calculated.

[0028] The soluble reagent was formulated according to the following proportions: cyclic (L-proline-L-valine) 15%, lignosulfonic acid 25%, and dibutyl phthalate 60%.

[0029] The preparation made according to the above ratio, diluted 100 times and sprayed, can reach 83% of the control effect of cucumber target spot disease; do contrast spraying with 30% cyclic (L-proline-L-valine) , its control efficiency can only reach 46%.

Embodiment 4

[0030] Embodiment 4 rice flax leaf spot

[0031] Spray once in the early stage of the disease, and 3 parallel treatment plots, each with an area of ​​20 square meters. The incidence was investigated before and 10 days after the application, and 5 points were randomly selected in each plot for sampling, and the percentage of the lesion area on each leaf of the whole plant to the leaf area was investigated, and the disease index and control effect were calculated.

[0032] Prepare the soluble reagent according to the following proportions: cyclic (L-proline-L-valine) 10%, niclosamide 10%, quality sulfonic acid 20%, and dibutyl phthalate 60%.

[0033] The preparation made according to the above ratio, diluted 100 times and sprayed, can reach 75% to the control effect of flax leaf spot of rice; implementation, its control efficiency can only reach 37%.

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Abstract

The invention discloses cyclic dipeptide and application of the cyclic dipeptide in the preparation of a bactericide and belongs to the field of plant protection. The cyclic dipeptide is 3-isopropyl-pyrrolopiperazine-2,5-dione. Pathogen controlled by the bactericide is one or more than one of a group consisting of Botrytis cinerea, corynespora cassiicola, Ascochyta citrullina, Colletotrichum orbiculare, Pyricularia grisea, Candida albicans, Pellicularia, Fusarium oxysporum, Rhizoctonia solani, xanthomonas oryzae, aspergillus flavus and Fusarium moniliforme. The invention reports bactericidal activity of cyclo(L-proline-L-valine) for the first time and provides a new thinking for research and development of the bactericide. As a bactericide, the cyclic dipeptide cyclo(L-proline-L-valine) isderived from a secondary metabolite of Pseudomonas syringae, belongs to a natural product, is easy to degrade in the environment, and is not easy to cause harm o the environment.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to a cyclic dipeptide and its application in the preparation of fungicides. Background technique [0002] In the process of agricultural production, plant pathogenic bacteria can easily infect crops and have a significant impact on the quality and yield of agricultural products. At present, the prevention and control of plant pathogenic bacteria in my country is mainly carried out through the application of chemical agents. Although chemical control has a better effect in a short period of time. However, pathogenic bacteria tend to develop resistance to chemicals, reducing the effect of chemical control. Moreover, chemical control can easily cause drug residues in agricultural products, pollute the ecological environment, and endanger human health. Therefore, it is necessary to seek safer and more efficient plant disease control technologies to meet human needs for safe and effici...

Claims

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Application Information

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IPC IPC(8): C07D487/04A01N43/90A01P3/00
CPCA01N43/90C07D487/04
Inventor 折冬梅李志康
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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