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Preparation method for lysozyme antibacterial film

An antibacterial film and lysozyme technology, applied in the field of antibacterial materials, can solve the problems of short antibacterial time, short sterilization time, complex process, etc., and achieve the effects of strengthening mechanical properties, enhancing sterilization durability, and ensuring solubility.

Inactive Publication Date: 2018-09-28
SHANDONG LUBEI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has become a trend to add natural antibacterial substances to replace chemically synthesized preservatives in food packaging bags. Applications in bags are greatly limited
[0003] Natural antibacterial agents need to be loaded into the carrier to solve the problems of strong pungent odor, low solubility and short effective antibacterial time. In the prior art, the following three methods are used to load natural antibacterial agents on the carrier 1) the natural antibacterial agent is directly mixed with the carrier; 2) the natural antibacterial agent is embedded with the carrier; 3) the natural antibacterial agent is combined with the carrier by cross-linking; the above three methods all have a single antibacterial agent, antibacterial The defects of poor effect and short antibacterial time limit, wherein, in the first method, the natural antibacterial agent is released too fast, and the bactericidal time limit is shorter; in the second method, the natural antibacterial agent is released too slowly, and the bactericidal effect is worse; the third method In the method, the preparation time is longer and the process is more complicated

Method used

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  • Preparation method for lysozyme antibacterial film
  • Preparation method for lysozyme antibacterial film

Examples

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Effect test

Embodiment 1

[0035] A preparation method of lysozyme antibacterial film, it comprises the following steps:

[0036] Step 1: In parts by weight, take 0.5 parts of cactus and 0.8 parts of aloe, wash, peel and chop them respectively, add 15 parts of distilled water, heat to 100°C and boil for 2 hours, filter while hot to obtain For the first filtrate and the first filter residue, add 0.01 part of nano-carbon powder and 0.005 part of nano-eggshell powder to the first filtrate and stir until they are evenly mixed to obtain the first mixture; take 0.3 part of peppermint through steam distillation The method is to obtain peppermint essential oil and distillation residue; add 0.003 parts of nano-carbon powder and 0.001 part of nano-eggshell powder to the peppermint essential oil and mix to obtain a second mixture;

[0037] Step 2. Mix the first filter residue and the distillation residue with 3 parts of beer and 10 parts of ethanol and stir evenly, then place it in an environment with a temperatur...

Embodiment 2

[0047] A preparation method of lysozyme antibacterial film, it comprises the following steps:

[0048] Step 1: In parts by weight, take 1 part of cactus and 1.2 parts of aloe, wash, peel and chop them respectively, add 20 parts of distilled water, heat to 110 ° C and boil for 3 hours, filter while hot, and obtain For the first filtrate and the first filter residue, add 0.05 parts of nano-carbon powder and 0.008 parts of nano-eggshell powder to the first filtrate and stir until they are evenly mixed to obtain the first mixture; take 0.5 parts of peppermint through steam distillation The method is to obtain peppermint essential oil and distillation residue; add 0.005 parts of nano-carbon powder and 0.003 parts of nano-eggshell powder to the peppermint essential oil and mix to obtain a second mixture;

[0049] Step 2. Mix the first filter residue and the distillation residue with 5 parts of beer and 15 parts of ethanol and stir evenly, then place it in an environment with a tempe...

Embodiment 3

[0059] A preparation method of lysozyme antibacterial film, it comprises the following steps:

[0060] Step 1. In parts by weight, take 0.8 parts of cactus and 1.0 part of aloe, wash, peel and chop them respectively, add 18 parts of distilled water, heat to 105°C and boil for 2.5 hours, filter while hot, Obtain the first filtrate and the first filter residue, add 0.03 parts of nano-carbon powder and 0.007 parts of nano-eggshell powder to the first filtrate and stir until they are evenly mixed to obtain the first mixture; get 0.4 parts of mint through steam Distillation method to obtain peppermint essential oil and distillation residue; add 0.004 part of nano-carbon powder and 0.002 part of nano-eggshell powder to the peppermint essential oil and mix to obtain the second mixture;

[0061] Step 2. Mix the first filter residue and the distillation residue with 4 parts of beer and 13 parts of ethanol and stir evenly, then place it in an environment with a temperature of -25°C and ...

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Abstract

The invention discloses a preparation method for a lysozyme antibacterial film. The method comprises the following steps: step 1, mixing an extraction liquid of cactus and aloe vera, nano carbon powder and nano eggshell powder to prepare a first mixture; and taking mint, and performing a water steam distilling method to obtain mint essential oil and distillation residues; step 2, processing firstfilter residues and the distillation residues by using beer and ethanol for a plurality of times, and collecting and processing the filtrates to prepare mixed powder; step 3, adding partial first mixture, second mixture and mixed powder and lysozyme into a gelatin aqueous solution to prepare a third mixed liquid; step 4, preparing a collagen sol, and adding the third mixed liquid and glycerin intothe collagen sol to obtain an antibacterial film solution; and step 5, performing casting film spreading on the antibacterial film solution, and performing drying to obtain the lysozyme antibacterialfilm. The preparation method disclosed by the invention is simple and easy to operate; and the prepared lysozyme antibacterial film has stronger mechanical properties, better antibacterial propertiesand superior slow-release properties.

Description

technical field [0001] The invention relates to the technical field of antibacterial materials. More specifically, the present invention relates to a kind of preparation method of lysozyme antibacterial film. Background technique [0002] The growth of pathogenic microorganisms in the process of food processing and storage is an important factor leading to food spoilage, so ensuring food safety and extending food shelf life are key scientific and technological issues in the food field that need to be solved urgently. It has become a trend to add natural antibacterial substances to replace chemically synthesized preservatives in food packaging bags. Applications in packaging bags are greatly limited. [0003] Natural antibacterial agents need to be loaded into the carrier to solve the problems of strong pungent odor, low solubility and short effective antibacterial time. In the prior art, the following three methods are used to load natural antibacterial agents on the carri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08K13/02C08K3/04C08K3/26C08K5/00C08K5/053C08J5/18C12P21/06C07K14/78C07K1/14
CPCC07K14/78C08J5/18C08J2389/00C08J2489/00C08K3/04C08K5/00C08K5/053C08K13/02C08K2003/265C12P21/06
Inventor 张春颖
Owner SHANDONG LUBEI PHARMA
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