Method for Improving the Oil Yield of Monopodella Cells
A single needle algae and oil technology, applied in the field of algae, can solve the problems of low oil production efficiency and high cultivation cost of algae, and achieve the effects of increasing oil production, promoting growth rate and increasing biomass
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Embodiment 1
[0028] The method for improving the oil production rate of Monopodella cells comprises the steps of:
[0029]Streak inoculation of Cellulomonas xanthogenes on the slant medium to obtain a single colony; pick a single colony and inoculate it on the primary seed medium for cultivation, and then culture it on the secondary seed medium to obtain Cellulomonas xanthogenes Bacteria seed liquid; the components of the slant medium are: yeast extract 50g / L, glucose 30g / L, agar 20g / L; the components of the primary seed medium and the secondary seed medium are: corn flour 30g / L, glucose 20g / L, ammonium nitrate 1g / L, magnesium sulfate heptahydrate 0.2g / L, potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, ferrous sulfate heptahydrate 0.1g / L L;
[0030] Pick Monotipella, plant them in a container containing growth medium, light intensity 5000lux, light-dark cycle 24h, light-dark ratio 14:10, cultivate at 28°C, grow to the logarithmic growth phase, and get Monotip...
Embodiment 2
[0035] The method for improving the oil production rate of Monopodella cells comprises the steps of:
[0036] Streak inoculation of Cellulomonas xanthogenes on the slant medium to obtain a single colony; pick a single colony and inoculate it on the primary seed medium for cultivation, and then culture it on the secondary seed medium to obtain Cellulomonas xanthogenes Bacteria seed liquid; the components of the slant medium are: yeast extract 50g / L, glucose 30g / L, agar 20g / L; the components of the primary seed medium and the secondary seed medium are: corn flour 30g / L, glucose 20g / L, ammonium nitrate 1g / L, magnesium sulfate heptahydrate 0.2g / L, potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, ferrous sulfate heptahydrate 0.1g / L L;
[0037] Pick Monotipella, plant them in a container containing growth medium, light intensity 5000lux, light-dark cycle 24h, light-dark ratio 14:10, cultivate at 28°C, grow to the logarithmic growth phase, and get Monoti...
Embodiment 3
[0048] The present invention has detected the influence of culture time on fatty acid components in oil:
[0049] The fatty acid composition of different algae species is very different, and the content of C16 and C18 is an important factor to determine the performance of oil. Taking Example 1 as an example, set the time gradient as 1, 3, 5, 7, 9 (d), and analyze the oil composition by GC-MS. The fatty acids produced by Monostipella during heterotrophic culture are mainly C16 and C18 . Such as figure 1 As shown, there is only a small amount of C16 in the early stage of culture, and as the culture time increases, the content of C16 increases, reaches the peak on the 5th day, and then decreases, but the decline is not large; C18 begins to be produced on the 3rd day , and then increased rapidly, and the increase was not obvious after the 7th day. Considering the cost and time of cultivation, it is most appropriate to choose to cultivate for about 7 days.
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