A composition having xanthine oxidase inhibitory activity
A technology for xanthine oxidase and inhibitory activity is applied in the field of preparing xanthine oxidase inhibitors and compositions with xanthine oxidase inhibitory activity, which can solve problems such as unreported, achieve high safety, and improve the addition of raw materials. value, the effect of promoting deep processing and utilization
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Embodiment 1
[0015] Example 1 Screening of Artemisia quinoa Leaf Extract for Inhibiting Xanthine Oxidase Effective Parts
[0016] (1) Preparation of each extraction part
[0017] Select fresh Artemisia quinoa leaves, dry them to constant weight, crush them through a 40-mesh sieve, then add 15 times the weight of 50% ethanol to reflux and extract 3 times, each extraction time is 1 hour, combine the extracts, and concentrate under reduced pressure to obtain an extract and placed in a separatory funnel.
[0018] Extract the extract with petroleum ether for 3 times, collect the extract, concentrate, and dry to obtain the petroleum ether part; the remaining extract is extracted with ethyl acetate for 3 times, collect the extract, concentrate, and dry to obtain ethyl acetate part; the remaining extract was extracted 3 times with n-butanol, and the extract was collected and concentrated and dried to obtain the n-butanol part; the remaining extract was dried, which was the water phase.
[0019] ...
Embodiment 2
[0024] Example 2 Preparation of Raspberry Extract and Detection of Its Inhibition of Xanthine Oxidase Activity
[0025] (1) Weigh the raspberry fruit, put it into a blender and pulverize and whip it to make it homogenate, adjust the pH of 70% ethanol solution to 2 with dilute hydrochloric acid as the extraction solvent, add 15 times to the raspberry homogenate weight of extraction solvent, ultrasonically extract for 1 hour, filter or centrifuge, collect the extract and concentrate under reduced pressure until there is no alcohol smell;
[0026] (2) Put the concentrated extract on the AB-8 type macroporous adsorption resin column, wash it with a hydrochloric acid aqueous solution with a pH of 2 at a speed of 0.25mL / s for 5h, and then wash it with a 70% ethanol solution with a pH of 2 at a rate of 2mL / min speed elution, collect 70% ethanol eluate and concentrate into a thick paste, and freeze-dry the thick paste to obtain.
[0027] Detect the inhibitory rate and IC of raspberr...
Embodiment 3
[0028] Preparation of Example 3 Composition and Detection of Inhibition of Xanthine Oxidase Activity
[0029] The Artemisia quinoa leaves ethyl acetate active fraction prepared in Example 1 and the raspberry extract prepared in Example 2 are respectively compatible in the proportions in Table 2, and then determined according to the method in Example 1 for each composition to xanthine Oxidase inhibition rate and IC 50 value.
[0030] See Table 2 for the results of the inhibition rate of each composition on xanthine oxidase at a concentration of 1 mg / mL.
[0031] Table 2 Inhibition rate of xanthine oxidase by different ratios of Artemisia quinoa leaf extract and raspberry extract composition
[0032] Raspberry Extract: Artemisia annua leaf ethyl acetate fraction Inhibition rate / % 10:1 37.63±3.3 8:1 40.73±2.2 4:1 44.25±0.8 2:1 48.14±2.6 1:1 58.47±3.2 1:2 64.18±0.5 1:4 66.07±2.0 1:8 72.48±1.2 1:10 69.40±1.5
[0033] A...
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