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Application of ntcngc1 gene in resistance to bacterial wilt in tobacco

A technology for resistance to bacterial wilt and bacterial wilt, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of the number of bacterial wilt resistance genes, the mechanism of action, and the unclear interaction mechanism between tobacco and bacterial wilt bacteria, and tobacco production. Loss and other issues, to achieve important application value and improve the effect of resistance

Active Publication Date: 2021-08-03
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tobacco bacterial wilt caused by Ralstonia solanacearum is a devastating soil-borne disease worldwide, causing huge losses in tobacco production
At present, there is little research on the molecular mechanism of tobacco resistance to bacterial wilt at home and abroad, and the number of resistance genes controlling bacterial wilt, the mechanism of action, and the interaction mechanism between tobacco and R. solanacearum are still unclear

Method used

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  • Application of ntcngc1 gene in resistance to bacterial wilt in tobacco
  • Application of ntcngc1 gene in resistance to bacterial wilt in tobacco
  • Application of ntcngc1 gene in resistance to bacterial wilt in tobacco

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Experimental program
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Embodiment Construction

[0014] 1. Embodiment

[0015] Experimental materials and reagents:

[0016] The highly pathogenic R. solanacearum was isolated and identified by the Key Laboratory of Molecular Genetics of the Tobacco Industry and subcultured in TTC medium. Tobacco bacterial wilt resistant variety DB101 and susceptible variety Honghua Dajinyuan were provided by the Fine Breeding Center of Guizhou Tobacco Science Research Institute. Cultivate in pots (peat soil: vermiculite: perlite = 1:1:1).

[0017] 1. Cloning of NtCNGC1 gene

[0018] Cloning primers CNGC1-F and CNGC1-R were designed according to the tobacco CNGC1 gene sequence (accession number: XM_016655150) (CNGC1 gene nucleotide sequence shown in SEQ ID NO: 1) in the GenBank database (Table 1). The total RNA of DB101 tobacco leaves was extracted and reverse-transcribed into cDNA, which was used as a template for PCR amplification, cloning and sequencing of tobacco CNGC1 gene. At the same time, genomic DNA was extracted from DB101 toba...

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Abstract

The invention discloses the application of a NtCNGC1 gene in tobacco bacterial wilt resistance, the nucleotide series of the NtCNGC1 gene is shown in SEQ ID NO: 1, and the overexpression of the NtCNGC1 gene in tobacco can significantly improve the resistance of tobacco to bacterial wilt. Resistance to bacterial wilt, the gene has very important application value in tobacco bacterial wilt resistance genetic engineering.

Description

technical field [0001] The invention relates to the application of NtCNGC1 gene in tobacco bacterial wilt resistance, belonging to the field of plant genetic engineering. Background technique [0002] Tobacco bacterial wilt caused by Ralstonia solanacearum is a devastating soil-borne disease worldwide, causing huge losses to tobacco production. At present, there is little research on the molecular mechanism of tobacco resistance to bacterial wilt at home and abroad, and the number of resistance genes controlling bacterial wilt, the mechanism of action, and the interaction mechanism between tobacco and R. solanacearum are still unclear. Analyzing and excavating possible bacterial wilt resistance genes in tobacco lines at the transcriptome level will not only deepen the understanding of the interaction between tobacco and R. solanacearum at the molecular level, but will also help the rational use of bacterial wilt resistance sources and breeding for disease resistance. Cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/8281
Inventor 林世锋余婧王仁刚任学良张洁余世洲张孝廉张吉顺
Owner GUIZHOU TOBACCO SCI RES INST