Application of copper nanocluster as fluorescent probe for specifically detecting the content of rifampicin in solution
A technology of copper nanoclusters and fluorescent probes, which is applied to the application field of metal nanoclusters in fluorescence sensing, can solve the problems of strict requirements for detection pretreatment, complex detection process, low detection sensitivity, etc., and achieves a wide detection linear range. , Low detection limit, good fluorescence performance
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Embodiment 1
[0027] The preparation of copper nanoclusters with glutathione as a stabilizer is carried out according to the following steps at room temperature:
[0028] (1) Preparation of 0.1 M copper chloride solution: weigh 1.7048 g CuCl 2 ∙2H 2 O was dissolved in 100 mL of high-purity water, fully dissolved for later use;
[0029] (2) Preparation of copper nanoclusters: at room temperature, 0.28 g glutathione was weighed and dissolved in 15 mL H 2 O, to which 450 μL CuCl was added 2 (0.1M), after fully reacting, add 0.1 g ascorbic acid (AA), then add 1 mL NaOH (1M), react for 1 h, until the white suspension is completely dissolved and turns into a light yellow clear solution, which proves that the copper nanoclusters form. By transmission electron microscopy (TEM) ( figure 1 ) It can be seen that the copper nanoclusters are uniformly dispersed and the particle size is small.
Embodiment 2
[0031] Copper nano-clusters are used as a method for the specific detection of rifampicin by fluorescent probes, which is characterized in that the steps are as follows:
[0032] (1) Preparation of 4 mM rifampicin mother solution: weigh 0.0329 g rifampicin and dissolve in 10 mL high-purity water, store at low temperature for later use;
[0033] (2) Preparation of a series of solutions of different concentrations of rifampicin:
[0034] Dilute the 4 mM rifampicin solution to different concentrations of 5 nM, 10 nM, 100 nM, 200 nM, and 1000 nM respectively, and make up the diluted solution to 4 mL;
[0035] (3) Evenly disperse the prepared copper nanoclusters based on glutathione as a stabilizer in high-purity water, prepare a detection system with a concentration of 1.2 mM and a volume of 4 mL, and measure the concentration at this time using a fluorescence spectrophotometer. Fluorescence intensity, under excitation at an excitation wavelength of 354 nm, the fluorescent probe ex...
Embodiment 3
[0041] 1. Refer to Example 1 for the preparation of copper nanoclusters using glutathione as a protective agent;
[0042] 2. Determination of excitation and emission spectra of copper nanoclusters stabilized with glutathione:
[0043] Disperse copper nanoclusters in high-purity water to measure the fluorescence excitation spectrum and fluorescence emission spectrum of the material, such as figure 2 As shown, the maximum excitation wavelength of copper nanoclusters is 354 nm, and under the excitation of the maximum excitation wavelength, the fluorescence emission wavelength is 632.02 nm.
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