Application of copper nanocluster as fluorescent probe for specifically detecting the content of rifampicin in solution

A technology of copper nanoclusters and fluorescent probes, which is applied to the application field of metal nanoclusters in fluorescence sensing, can solve the problems of strict requirements for detection pretreatment, complex detection process, low detection sensitivity, etc., and achieves a wide detection linear range. , Low detection limit, good fluorescence performance

Inactive Publication Date: 2018-10-19
TIANJIN NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the easiest way to assess the dose of rifampicin is to measure the amount of rifampicin metabolites in the patient's urine, however, this result may be inaccurate
Because the increase in intake and the accumulation of drugs in the liver will increase the risk of hepatotoxicity, many new methods for detecting the content of rifampicin have been established, which are conventional drug analysis methods, spectrophotometry, and electrophysiology. Chemical methods and high-performance liquid chromatography, etc., but due to the complexity of the detection process, the harsh requirements of the pre-test treatment, the high requirements of the detection purity, and the low detection sensitivity, the wide application of these methods is limited.

Method used

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  • Application of copper nanocluster as fluorescent probe for specifically detecting the content of rifampicin in solution
  • Application of copper nanocluster as fluorescent probe for specifically detecting the content of rifampicin in solution
  • Application of copper nanocluster as fluorescent probe for specifically detecting the content of rifampicin in solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of copper nanoclusters with glutathione as a stabilizer is carried out according to the following steps at room temperature:

[0028] (1) Preparation of 0.1 M copper chloride solution: weigh 1.7048 g CuCl 2 ∙2H 2 O was dissolved in 100 mL of high-purity water, fully dissolved for later use;

[0029] (2) Preparation of copper nanoclusters: at room temperature, 0.28 g glutathione was weighed and dissolved in 15 mL H 2 O, to which 450 μL CuCl was added 2 (0.1M), after fully reacting, add 0.1 g ascorbic acid (AA), then add 1 mL NaOH (1M), react for 1 h, until the white suspension is completely dissolved and turns into a light yellow clear solution, which proves that the copper nanoclusters form. By transmission electron microscopy (TEM) ( figure 1 ) It can be seen that the copper nanoclusters are uniformly dispersed and the particle size is small.

Embodiment 2

[0031] Copper nano-clusters are used as a method for the specific detection of rifampicin by fluorescent probes, which is characterized in that the steps are as follows:

[0032] (1) Preparation of 4 mM rifampicin mother solution: weigh 0.0329 g rifampicin and dissolve in 10 mL high-purity water, store at low temperature for later use;

[0033] (2) Preparation of a series of solutions of different concentrations of rifampicin:

[0034] Dilute the 4 mM rifampicin solution to different concentrations of 5 nM, 10 nM, 100 nM, 200 nM, and 1000 nM respectively, and make up the diluted solution to 4 mL;

[0035] (3) Evenly disperse the prepared copper nanoclusters based on glutathione as a stabilizer in high-purity water, prepare a detection system with a concentration of 1.2 mM and a volume of 4 mL, and measure the concentration at this time using a fluorescence spectrophotometer. Fluorescence intensity, under excitation at an excitation wavelength of 354 nm, the fluorescent probe ex...

Embodiment 3

[0041] 1. Refer to Example 1 for the preparation of copper nanoclusters using glutathione as a protective agent;

[0042] 2. Determination of excitation and emission spectra of copper nanoclusters stabilized with glutathione:

[0043] Disperse copper nanoclusters in high-purity water to measure the fluorescence excitation spectrum and fluorescence emission spectrum of the material, such as figure 2 As shown, the maximum excitation wavelength of copper nanoclusters is 354 nm, and under the excitation of the maximum excitation wavelength, the fluorescence emission wavelength is 632.02 nm.

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Abstract

The invention discloses application of a copper nanocluster as a fluorescent probe for specifically detecting the content of rifampicin in a solution. The invention achieves a label-free, high-sensitivity and selective detection method of the rifampicin in a fluorescence quenching turn-off mode; the method is simple and fast; by the method, the trace detection of the rifampicin can be achieved, the detection linear range is wide, and the detection limit is low. The copper nanocluster has relatively good detection sensitivity and selectivity, and has a very good application prospect in biological system detection, clinical application and the like.

Description

[0001] This patent has been supported by the National Natural Science Foundation of China (21375095), Tianjin Natural Science Foundation Youth Project (No.17JCQNJC05800), Tianjin Normal University Doctoral Fund Project (No.52XB1510), Tianjin Normal University "Inorganic-Organic Hybrid Functional Materials" The Key Laboratory of the Ministry of Chemistry Education", the "Tianjin Key Laboratory of Functional Molecular Structure and Performance" open fund project and the "Future Thousand Talents Program" project of Tianjin Normal University. technical field [0002] The invention belongs to the application field of metal nano-clusters in fluorescence sensing, and specifically relates to the application of copper nano-clusters in fluorescence quenching "turn off" mode, label-free, efficient and selective detection of rifampicin content in complex systems. Background technique [0003] Tuberculosis, an infectious disease affecting millions of people worldwide, is caused by many st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李妍吴晓曼冯子硕张菲
Owner TIANJIN NORMAL UNIVERSITY
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