Application of juglone in treatment of human liver cancer

A technology of jugone and liver cancer, applied in the field of jugone in the treatment of human liver cancer, can solve the problems of poor clinical treatment effect, large side effects, hidden onset, etc., and achieve the effects of improving biological efficacy, preventing oxidative degradation, and accelerating the dissolution rate.

Inactive Publication Date: 2018-10-26
TONGHUA NORMAL UNIV
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  • Abstract
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Problems solved by technology

In addition, liver cancer has the characteristics of insidious onset and rapid progression, so far the clinical treatment effect is still poor. Surgery and chemotherapy are still the mainstream cancer treatment methods at present, but the metastatic nature of the tumor may lead to tumor recurrence after surgical treatment, and intrahepatic recurrence after liver cancer surgery is also the main cause of death for liver cancer patients. The side effects of drugs on the body itself are relatively large, so people turn their attention to traditional Chinese medicine to prepare anti-tumor drugs

Method used

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  • Application of juglone in treatment of human liver cancer
  • Application of juglone in treatment of human liver cancer
  • Application of juglone in treatment of human liver cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation steps of juglone include: pretreatment, leaching, and purification, specifically including the following steps:

[0027]Pretreatment: pick walnuts after they are ripe, select smooth, healthy and uniform green walnut peels, dry them at 50°C for later use, grind them with a traditional Chinese medicine grinder, and pass through a 100-mesh sieve to obtain dry walnut green peels; according to the ratio of material to liquid 1:8 The ratio is mixed evenly with isopropanol. In isopropanol, 3.2% n-butanol and 0.45‰ of 4-benzyl-2-oxazolone are pre-dissolved, and 4-benzyl-2-oxazolone contains 5% The (R)-4-benzyl-2-oxazolone was reacted at 31°C for 3 hours, filtered, centrifuged at 5000r / min for 25 minutes, dried and crushed to obtain degreased dry walnut green peel powder; walnut green peel was dried and crushed After the powder is obtained, it is convenient for the subsequent extraction of juglone; isopropanol can remove part of the fat in the walnut green peel d...

Embodiment 2

[0036] Effects of different concentrations of juglone on the morphology of HepG2 cell lines:

[0037] The HepG2 cell line without juglone treatment began to adhere to the wall 4-8 hours after inoculation, the color was transparent, and it was a regular polygon with certain protrusions (such as figure 1 -A). After 48 hours of inoculation and culture, most of the HepG2 cell lines in the 5 μM and 10 μM treatment groups were still in a viable state, adhered loosely, and some of them fell off. (Such as figure 1 -B, 1-C). 25μM, 50μM and 100μM treatment groups HepG2 cell strains were significantly retracted and rounded, the cells had been detached from the bottom, and there were a large number of dead cells suspended in the culture medium (such as figure 1 -D, 1-E, 1-F), and the above changes were more obvious with the increase of juglone concentration. It shows that juglone does have inhibitory effect on HepG2 cell line in the range of 5-10 μM, but it is not obvious. When the...

Embodiment 3

[0039] MTT assay was used to detect the effect of different concentrations of juglone (0, 5, 10, 25, 50, 100 μM) on the activity of HepG2 cells:

[0040] The results of MTT method are shown in Table 1. In the control group, the activity of HepG2 cell lines increased with time, and the OD value of the juglone treatment group was lower than that of the control group, which indicated that juglone had an inhibitory effect on HepG2 cell lines. The t-test between groups showed that 25 μM, 50 μM and 100 μM had significant inhibitory effects on the activity of HepG2 cell lines (P0.05).

[0041] Table 1 Effects of different concentrations of juglone on the growth and proliferation of HepG2 cells ( ±s)

[0042]

[0043] Note: Compared with the control group, the t test was carried out between the groups, * P<0.05.

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Abstract

The invention discloses application of juglone in the treatment of human liver cancer, wherein the juglone can inhibit the proliferation and invasion of human liver cancer cells, can inhibit the growth of tumor cells in the treatment of human liver cancer, and can be used for preparing anti-tumor drugs; meanwhile, the invention also discloses an extraction method of the juglone, firstly, walnut green tangerine peel is dried and crushed, then the walnut green tangerine peel is leached with ethanol solution under the aid of double-frequency ultrasonic waves, and finally, the walnut green tangerine peel is purified by D101 resin to obtain a juglone pure product. The juglone has the advantages of inhibiting proliferation and invasion of HepG2 cells, inhibiting the growth of tumor cells in thetreatment of human liver cancer, and directly killing tumor cells and inhibiting the invasion. The method of extracting the juglone from walnut green tangerine peel is simple and easy. The yield and the biological efficacy of the juglone can be well preserved, and oxidative degradation of the juglone and the derivatives thereof can be prevented.

Description

technical field [0001] The invention relates to the application field of juglone, in particular to the application of juglone in the treatment of human liver cancer. technical background [0002] Liver cancer is a high-incidence malignant tumor that seriously threatens human health. Its morbidity and fatality rate ranks fifth and second respectively among malignant tumors. Liver cancer is mostly in the middle and late stage when it is clinically diagnosed, and the opportunity for surgery will be delayed. In addition, liver cancer has the characteristics of insidious onset and rapid progression, so far the clinical treatment effect is still poor. Surgery and chemotherapy are still the mainstream tumor treatment methods at present, but the metastatic nature of the tumor may lead to tumor recurrence after surgical treatment, and intrahepatic recurrence after liver cancer surgery is also the main cause of death for liver cancer patients. Drugs have relatively large side effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00C07C50/32C07C46/10
CPCA61K31/122A61P35/00C07C46/10C07C50/32
Inventor 冯颖汪家瑶朱俊义顾地周孙忠林苗婷婷赵云龙曲广男
Owner TONGHUA NORMAL UNIV
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