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Glucose composition, microbial fermentation raw material, and method for producing chemical product

A technology of microbial fermentation and composition, applied in the directions of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of low fermentation yield and difficult fermentation, and achieve the effect of reducing manufacturing costs

Pending Publication Date: 2018-10-26
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sugars made from cellulose-containing biomass contain xylose, which is a refractory pentose sugar, in addition to glucose, and organic acids such as acetic acid and HMF (hydroxymethylfurfural) as fermentation inhibitors. , coumaric acid, ferulic acid, vanillin, etc., so when used as a microbial fermentation raw material, there is a problem that the fermentation yield is low

Method used

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  • Glucose composition, microbial fermentation raw material, and method for producing chemical product
  • Glucose composition, microbial fermentation raw material, and method for producing chemical product
  • Glucose composition, microbial fermentation raw material, and method for producing chemical product

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Embodiment

[0060] Examples are given below to describe the present invention in detail. However, the present invention is not limited by these Examples.

reference example 1

[0061] (Reference Example 1) Preparation of Yeast (Saccaromyces cereveciae) for Ethanol Fermentation Evaluation Test

[0062] As the ethanol-fermenting microorganism, Saccharomyces cereveciae (strain of red wine yeast OC2, NBRC2260) was used. YPD medium (the peptone, yeast extract concentrate, and glucose concentrate were sterilized in an autoclave at 121°C for 20 minutes, and prepared into 20 g / L peptone, 10 g / L yeast extract, and 10 g / L glucose. Culture medium) 2 mL was added to a test tube, and a platinum loop was used to inoculate yeast colonies formed on YPD agar medium plate culture (30° C., 1 to 2 days) in a clean bench. This was cultured with reciprocating shaking at 50°, 30° C., and 120 rpm using a shaker (TAITEC Co., Ltd., BIO-SHAKER BR-40LF) for 24 hours to obtain a preculture solution. The preculture solution was centrifuged at 15,000×g for 5 minutes at 4° C., the supernatant was removed, and suspended in 2 mL of physiological saline to obtain a preculture cell fl...

reference example 2

[0063] (Reference example 2) Measurement of sugar and ethanol concentration

[0064] The concentrations of glucose and xylose contained in the sugar solution, and the concentration of ethanol produced by fermentation were quantified by comparison with standard samples under the HPLC conditions shown below.

[0065] Column: Shodex SH1011 (manufactured by Showa Denko Co., Ltd.)

[0066] Mobile phase: sulfuric acid 5mM (flow rate 0.6mL / min)

[0067] Reaction solution: no

[0068] Detection method: RI (differential refractive index)

[0069] Temperature: 65°C.

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Abstract

The present invention is a glucose composition characterized by containing, as equivalents in an aqueous solution of the composition having a glucose concentration of 100 g / L, 0-25 g / L of xylose, 0.1-10 g / L of acetic acid, 0.15-2.0 g / L of coumaric acid, and 0.007-0.28 g / L of ferulic acid. By using the glucose composition as a raw material in a method for producing a chemical product by microbial fermentation, the yield of the chemical product can be raised.

Description

technical field [0001] The present invention relates to a glucose composition, a microbial fermentation raw material for improving the efficiency of microbial fermentation, and a method for producing chemicals using microbial fermentation. Background technique [0002] Fermentative production processes of sugar-based chemicals are used in the production of various industrial raw materials. As the sugar used as the raw material for this fermentation, sugar derived from edible raw materials such as sugar cane, starch, and sugar beet is currently used industrially. Raw sugar obtained from sugarcane and sugar beet contains sucrose as a main component, and becomes a high-purity sugar solution with a sucrose purity of 95% or more. On the other hand, the crystallized mother liquor produced in the process of obtaining raw sugar appears on the market as molasses, but it is a mixture of sucrose, glucose, and fructose, and contains various salts, proteins, etc. as impurities, so It b...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12P13/08C12N1/16C12N1/20C12P1/00C12P7/06C12P7/46C12P7/56C12P13/00
CPCC12N1/20C12P7/46C12P7/56C12P13/08Y02E50/10C12P13/001C12P7/10C12P19/02C12P19/14C12N1/16C12N1/00C12P13/00C12P1/00C12P7/06C12P1/02C12P1/04
Inventor 船田茂行栗原宏征山田胜成
Owner TORAY IND INC