Preparation method of hydrogel capable of continuously inducing cartilage differentiation and connecting with bone base

A hydrogel and cartilage technology, which is applied in the fields of pharmaceutical formulation, medical science, prosthesis, etc., can solve problems such as difficulty in connecting with bone base, failure of hydrogel to recognize slow-release growth factors, failure of hydrogel to induce cartilage differentiation, etc.

Active Publication Date: 2018-10-30
华清智美(深圳)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the technical problem to be solved by the present invention is that the hydrogel cannot recognize slow-release growth factors, the conventional hydrogel has poor mechanical properties, the hydrogel cannot induce cartilage differentiation, and it is difficult to connect with the bone base
[0008] The technical solution of the pre...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020]a) Under sterile conditions, first adopt the layer-by-layer self-assembly method to mineralize and deposit a layer of calcium phosphate shell on the surface of bone marrow mesenchymal stem cells to protect the bone marrow mesenchymal stem cells so as to reduce the impact of the polymerization process on the bone marrow space. Injury to mesenchymal stem cells; weigh 0.005g sodium silicate, 5g acrylamide, 0.5g sodium alginate, and N,N′-methylenebisacrylamide with a mass percentage of 0.03% acrylamide, and dissolve them in 50ml deionized water , stirring and dissolving evenly, after static defoaming, casting solution A was obtained, and then transforming growth factor-β1 and bone marrow mesenchymal stem cells wrapped in calcium phosphate shell were dissolved and dispersed in casting solution A to obtain casting solution B , dissolving trisodium phosphate in casting solution A to obtain casting solution C, placing casting solution C in a sterile container for later use, casti...

Embodiment 2

[0027] a) Under sterile conditions, first use layer-by-layer self-assembly method to mineralize and deposit a layer of calcium phosphate shell on the surface of adipose stem cells to protect the adipose stem cells to reduce the damage to the adipose stem cells during the polymerization reaction; weigh 2g Sodium silicate, 15g of acrylamide, 2g of sodium alginate, and N,N'-methylenebisacrylamide with a mass percentage of 0.30% of acrylamide are dissolved together in 100ml of deionized water, stirred and dissolved evenly, and left to stand for defoaming to obtain Casting solution A, and then respectively dissolving and dispersing transforming growth factor-β2 and fat stem cells wrapped in calcium phosphate shell in casting solution A to obtain casting solution B, dissolving sodium dihydrogen phosphate in casting solution A to obtain Casting solution C, the casting solution C is placed in a sterile container for later use, and the casting solution B containing transforming growth f...

Embodiment 3

[0034] a) Under sterile conditions, first adopt layer-by-layer self-assembly method to mineralize and deposit a layer of calcium phosphate shell on the surface of the cord blood stem cells to protect the cord blood stem cells so as to reduce the damage to the cord blood stem cells during the polymerization process; Weigh 1.0g of sodium silicate, 10g of acrylamide, 1g of sodium alginate, and N,N'-methylenebisacrylamide with a mass percentage of 0.10% of acrylamide, and dissolve them together in 80ml of deionized water, stir to dissolve evenly, and let stand Casting solution A was obtained after defoaming, and then transforming growth factor-β3 and umbilical cord blood stem cells wrapped in calcium phosphate shell were dissolved and dispersed in casting solution A to obtain casting solution B, and potassium dihydrogen phosphate was dissolved in casting solution The casting solution C is obtained from the membrane solution A, and the casting solution C is placed in a sterile conta...

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Abstract

The invention provides a preparation method of hydrogel capable of continuously inducing cartilage differentiation and connecting with a bone base. The preparation method includes: covering the surface of stem cells with a layer of calcium phosphate casing, dissolving soluble phosphate, sodium silicate, acrylamide, a cross-linking agent and sodium alginate in water to obtain casting membrane liquid A, dispersing the stem cells covered in the calcium phosphate casing and growth factors in the casting membrane liquid A to obtain casting membrane liquid B, uniformly scraping the casting membraneliquid A on a glass plate A by a glass rod which is twisted with a copper wire, pouring the casting membrane liquid B on the casting membrane liquid A, uniformly scraping the casting membrane liquid Bby the glass rod twisted with the copper wire, initiating acrylamide polymerization by ultraviolet, subjecting the casting membrane liquid A and the casting membrane liquid B, along with the glass plate A, to calcium ion crosslinking, soaking the glass plate A in aqueous gluconic acid-delta-lactone solution, eluting the growth factors to obtain a high-strength hydrogel membrane which is providedembedded stem cells in a surface layer and is capable of inducing cartilage differentiation, tearing the high-strength hydrogel membrane off the glass plate A, and subjecting the high-strength hydrogel membrane to calcium ion crosslinking to obtain the hydrogel which comprises calcium phosphate in a lower layer and is capable of connecting with the bone base.

Description

technical field [0001] The invention relates to a preparation method of a hydrogel capable of sustainably inducing cartilage differentiation and connecting with a bone base, and belongs to the fields of biomaterials and tissue engineering. Background technique [0002] Osteoarthritis has become the number one disabling disease in the world, but because there are no blood vessels in the cartilage to provide nutrition, the migration ability of chondrocytes is poor, and articular cartilage cannot repair itself after damage. At present, clinical treatment of articular cartilage injuries is mainly based on surgery, including osteotomy, arthroscopic repair, autologous cartilage transplantation, and arthrodesis. These treatments can only relieve pain, but cannot replace the biological properties of cartilage, let alone promote cartilage regeneration. Traditional therapy is expensive, and there will be inflammation and rejection, etc., and the long-term treatment effect is controve...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/16A61L27/12A61L27/02A61L27/38A61L27/50A61L27/52A61L27/54
CPCA61L27/025A61L27/12A61L27/16A61L27/20A61L27/3834A61L27/3852A61L27/50A61L27/52A61L27/54A61L2300/112A61L2300/414A61L2400/12A61L2430/06C08L5/04C08L33/26
Inventor 赵孔银张天烨王力鑫吕霈雨朱敦皖张琳华樊帆魏俊富刘聪聪
Owner 华清智美(深圳)生物科技有限公司
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