Preparation method of hydrogel capable of continuously inducing cartilage differentiation and connecting with bone base

Abstract

The invention provides a preparation method of hydrogel capable of continuously inducing cartilage differentiation and connecting with a bone base. The preparation method includes: covering the surface of stem cells with a layer of calcium phosphate casing, dissolving soluble phosphate, sodium silicate, acrylamide, a cross-linking agent and sodium alginate in water to obtain casting membrane liquid A, dispersing the stem cells covered in the calcium phosphate casing and growth factors in the casting membrane liquid A to obtain casting membrane liquid B, uniformly scraping the casting membraneliquid A on a glass plate A by a glass rod which is twisted with a copper wire, pouring the casting membrane liquid B on the casting membrane liquid A, uniformly scraping the casting membrane liquid Bby the glass rod twisted with the copper wire, initiating acrylamide polymerization by ultraviolet, subjecting the casting membrane liquid A and the casting membrane liquid B, along with the glass plate A, to calcium ion crosslinking, soaking the glass plate A in aqueous gluconic acid-delta-lactone solution, eluting the growth factors to obtain a high-strength hydrogel membrane which is providedembedded stem cells in a surface layer and is capable of inducing cartilage differentiation, tearing the high-strength hydrogel membrane off the glass plate A, and subjecting the high-strength hydrogel membrane to calcium ion crosslinking to obtain the hydrogel which comprises calcium phosphate in a lower layer and is capable of connecting with the bone base.

Description

technical field [0001] The invention relates to a preparation method of a hydrogel capable of sustainably inducing cartilage differentiation and connecting with a bone base, and belongs to the fields of biomaterials and tissue engineering. Background technique [0002] Osteoarthritis has become the number one disabling disease in the world, but because there are no blood vessels in the cartilage to provide nutrition, the migration ability of chondrocytes is poor, and articular cartilage cannot repair itself after damage. At present, clinical treatment of articular cartilage injuries is mainly based on surgery, including osteotomy, arthroscopic repair, autologous cartilage transplantation, and arthrodesis. These treatments can only relieve pain, but cannot replace the biological properties of cartilage, let alone promote cartilage regeneration. Traditional therapy is expensive, and there will be inflammation and rejection, etc., and the long-term treatment effect is controve...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the technical problem to be solved by the present invention is that the hydrogel cannot recognize slow-release growth factors, the conventional hydrogel has poor mechanical properties, the hydrogel cannot induce cartilage differentiation, and it is difficult to connect with the bone base

[0008] The technical solution of the present invention to solve the problems that the hydrogel cannot recognize slow-release growth factors, conventional hydrogels have poor mechanical properties, hydrogels cannot induce cartilage differentiation, and it is difficult to connect with the bone base is to provide a sustainable induction of cartilage differentiation Preparation method of hydrogel connected with bone substrate