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Tissue engineering cartilage framework as well as preparation method and application thereof

A tissue engineering and cartilage technology, applied in the fields of medicine and biomedical engineering, can solve the problems of insufficient mechanical strength of regenerated cartilage, damage to the structural integrity of cartilage acellular matrix, etc.

Inactive Publication Date: 2018-11-02
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these two methods can effectively solve the problem of complete decellularization and inoculation of seeded cells in the deep layer of cartilage tissue, it destroys the structural integrity of the decellularized matrix of cartilage, resulting in insufficient mechanical strength of regenerated cartilage

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  • Tissue engineering cartilage framework as well as preparation method and application thereof
  • Tissue engineering cartilage framework as well as preparation method and application thereof
  • Tissue engineering cartilage framework as well as preparation method and application thereof

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preparation example Construction

[0034] The preparation method of the tissue engineering cartilage scaffold provided by the invention comprises steps:

[0035] The first step is to perform laser drilling on the natural cartilage tissue to obtain microporous cartilage tissue. The output power in the drilling parameters is 3-20 watts, the pulse time is 10-30 milliseconds, and the spot diameter is 100-500 microns. The number of repetitions is 2-8 times; in the second step, the microporous cartilage tissue that has been drilled by the laser is subjected to decellularization.

[0036] In the above first step, the output power is preferably 8-12 watts, more preferably 10 watts.

[0037] In the first step above, the pulse time in the punching parameters is preferably 16-18 milliseconds, more preferably 17 milliseconds.

[0038] In the above first step, the spot diameter is preferably 250 microns.

[0039] In the first step above, it is preferable to repeat punching 3-5 times, more preferably 4 times.

[0040] In ...

Embodiment 1

[0065] Fabrication of laser-drilled decellularized tracheal scaffolds

[0066] 1. Obtaining Allogeneic Tracheal Tissue

[0067] New Zealand white rabbits were sacrificed with an air plug, and a 3-4 cm trachea was cut from the neck to expose the trachea and placed in phosphate buffered solution (PBS) at 4°C. Accompanying muscle and adipose tissue are carefully removed to obtain allogeneic tracheal tissue (still tubular, see figure 1 A) and placed in PBS at 4°C.

[0068] 2. Laser drilling the trachea and screening the optimal parameters

[0069] The trachea is supported by a support tube that is almost equal to the inner diameter of the trachea (or a little smaller), and then rotated at a certain rate, C0 2 A laser puncher (synrad-6500, WA98275)) emits a laser to punch a hole on the surface of the trachea ( figure 1 B). When the parameter is set to an output power below 5W, even if the pulse time and repetition times remain unchanged, the micropore depth may only reach 1 / 2 ...

Embodiment 2

[0089] In vivo / in vitro construction of tissue engineered tracheal cartilage grafts

[0090] 1. Isolation and Culture of Chondrocytes

[0091] The conventional method is to isolate chondrocytes from rabbit auricular cartilage and carry out in vitro expansion and culture to P2 generation.

[0092] 2. In vivo and in vitro regeneration of tissue-engineered tracheal cartilage

[0093] Collect a sufficient amount of second-generation chondrocytes, carefully and evenly inoculate the cells on the outer surface of the acellular matrix scaffold (sheet and tube), culture at 37°C, 5% CO2, and saturated humidity conditions, and change the medium every 2 days. Two weeks later, some of the cell-material complexes were implanted into nude mice and cultured for 12 weeks; the rest of the cell-material complexes were cultured in vitro for up to 8 weeks. It was found that, no matter in vivo or in vitro, tissue engineered cartilage tissue with porcelain white and sufficient mechanical strength ...

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Abstract

The invention discloses a tissue engineering cartilage framework as well as a preparation method and a application thereof. The preparation method of the tissue engineering cartilage framework comprises the following steps of (1) performing laser hole forming on natural cartilage tissues to obtain laser microporous cartilage tissues; (2) performing decellularization on the laser microporous cartilage tissues to obtain the tissue engineering cartilage framework.

Description

technical field [0001] The present invention relates to the fields of medicine and biomedical engineering, and more particularly relates to a tissue engineering cartilage scaffold and its preparation method and application. Background technique [0002] Cartilage defect is a common clinical disease. Due to the limited self-repair ability of cartilage tissue, the repair of various types of cartilage injuries has always been a difficult problem in clinical treatment. In recent years, the rapid development of tissue engineering technology provides a new idea to solve this problem. The three elements of tissue engineering include seed cells, scaffold materials and growth factors, among which ideal scaffold materials are crucial to the successful construction of tissue engineered cartilage. However, most scaffold materials currently used in cartilage tissue engineering research lack structural properties and biochemical compositions similar to natural cartilage. The cartilage ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3612A61L27/3654A61L27/3687A61L27/50A61L2430/06A61L2430/40
Inventor 徐勇段亮李丹周广东
Owner SHANGHAI PULMONARY HOSPITAL
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