Moringa leaf extract with blood sugar lowering activity and preparation method thereof

A technology of Moringa oleifera leaves and extracts, applied in the field of deep processing and high value of Moringa oleifera leaves, to achieve the effect of good hypoglycemic activity

Active Publication Date: 2018-11-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is no relevant report on

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  • Moringa leaf extract with blood sugar lowering activity and preparation method thereof
  • Moringa leaf extract with blood sugar lowering activity and preparation method thereof
  • Moringa leaf extract with blood sugar lowering activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:

[0058] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through an 80-mesh sieve to obtain dried leaves of Moringa oleifera.

[0059] (2) Alkaline treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:10g / mL evenly, add sodium hydroxide to adjust the pH to 8.0, keep the temperature at 45°C, and stir at 120r / min for 30min to obtain a suspension Liquid 1.

[0060] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 according to 4% dry leaf powder of Moringa oleifera, among which, the amount of papain accounts for 70% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 30% of the total enzyme amount , constant temperature at 45°C, stirring at 120r / min, enzymatic hydrolysis for 4h, and suspension 2 was obtained.

[0061] (4) Cellulase enzymatic hydr...

Embodiment 2

[0071] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:

[0072] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through an 80-mesh sieve to obtain dried leaves of Moringa oleifera.

[0073] (2) Alkaline treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:17.5g / mL evenly, add sodium hydroxide to adjust the pH to 8.5, keep the temperature at 50°C, and stir at 150r / min for 45min to obtain a mixture Suspension 1.

[0074] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 according to 6% dry leaf powder of Moringa oleifera, among which, the amount of papain accounts for 80% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 20% of the total enzyme amount , constant temperature at 50°C, stirring at 150r / min, enzymatic hydrolysis for 8h, and suspension 2 was obtained.

[0075] (4) Cellulase enzymatic h...

Embodiment 3

[0085] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:

[0086] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through a 100-mesh sieve to obtain dried leaves of Moringa oleifera.

[0087] (2) Alkali treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:25g / mL evenly, add sodium hydroxide to adjust the pH to 9.0, keep the temperature at 55°C, and stir at 180r / min for 60min to obtain a suspension Liquid 1.

[0088] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 at the rate of 8% dry leaf powder of Moringa oleifera, in which the amount of papain accounts for 90% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 10% of the total enzyme amount , constant temperature at 55°C, stirring at 180r / min, enzymatic hydrolysis for 12h, and suspension 2 was obtained.

[0089] (4) Cellulase enzymatic hydroly...

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Abstract

The invention discloses a moringa leaf extract with blood sugar lowering activity and a preparation method thereof. The preparation method comprises the following steps: crushing and sieving, performing alkali treatment, performing protease enzymolysis, performing cellulase enzymolysis, performing high-pressure homogenization, performing high-temperature extraction, performing low-temperature high-speed centrifugation, performing ultrafiltration separation and concentration, performing protease enzymolysis, performing low-temperature high-speed centrifugation, performing secondary ultrafiltration separation and concentration, performing freeze drying and the like to obtain the moringa leaf extract with the blood sugar lowering activity. The extract has the polysaccharide content of 30% orabove; an animal experimental model of a type-2 diabetic rat verifies that the extract has good blood sugar lowering activity and can effectively reduce the fasting blood glucose of the type-2 diabetic rat and enhance the oral glucose tolerance dosage thereof; the acting effect of the extract is similar to that of metformin with the same dosage. An extracting technology is simple, the whole technological process can meet a food-grade requirement, and the moringa leaf extract can be applied to the field of medicines, health-care products, food and the like.

Description

technical field [0001] The invention belongs to the field of deep processing and high value of moringa leaves, and in particular relates to a moringa leaf extract with hypoglycemic activity and a preparation method thereof. Background technique [0002] At present, with the improvement of living standards, the incidence of diabetes is increasing year by year, and it has become the third chronic disease that seriously endangers human health after tumors and cardiovascular and cerebrovascular diseases. Due to the great harm that diabetes has brought to human beings, the World Health Organization called it the disaster of the 21st century. In 2015, the number of people with diabetes worldwide rose to 415 million. Therefore, the development of plant extracts with hypoglycemic activity and high safety has significant social and economic benefits. [0003] Moringa ( Moringa oleifera Lam. ), native to India, is a perennial tropical deciduous tree of the Moringaceae family Moring...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P3/10A23L33/105A61K127/00
CPCA23V2002/00A61K36/185A61K2236/15A61K2236/19A61K2236/331A61K2236/51A61K2236/53A23L33/105A61P3/10A23V2200/328
Inventor 赵谋明李巧琳林恋竹苏国万
Owner SOUTH CHINA UNIV OF TECH
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