Moringa leaf extract with blood sugar lowering activity and preparation method thereof
A technology of Moringa oleifera leaves and extracts, applied in the field of deep processing and high value of Moringa oleifera leaves, to achieve the effect of good hypoglycemic activity
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Embodiment 1
[0057] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:
[0058] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through an 80-mesh sieve to obtain dried leaves of Moringa oleifera.
[0059] (2) Alkaline treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:10g / mL evenly, add sodium hydroxide to adjust the pH to 8.0, keep the temperature at 45°C, and stir at 120r / min for 30min to obtain a suspension Liquid 1.
[0060] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 according to 4% dry leaf powder of Moringa oleifera, among which, the amount of papain accounts for 70% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 30% of the total enzyme amount , constant temperature at 45°C, stirring at 120r / min, enzymatic hydrolysis for 4h, and suspension 2 was obtained.
[0061] (4) Cellulase enzymatic hydr...
Embodiment 2
[0071] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:
[0072] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through an 80-mesh sieve to obtain dried leaves of Moringa oleifera.
[0073] (2) Alkaline treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:17.5g / mL evenly, add sodium hydroxide to adjust the pH to 8.5, keep the temperature at 50°C, and stir at 150r / min for 45min to obtain a mixture Suspension 1.
[0074] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 according to 6% dry leaf powder of Moringa oleifera, among which, the amount of papain accounts for 80% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 20% of the total enzyme amount , constant temperature at 50°C, stirring at 150r / min, enzymatic hydrolysis for 8h, and suspension 2 was obtained.
[0075] (4) Cellulase enzymatic h...
Embodiment 3
[0085] A Moringa oleifera leaf extract with hypoglycemic activity, specifically comprising the steps of:
[0086] (1) Crushing and sieving: crush the dried leaves of Moringa oleifera and pass through a 100-mesh sieve to obtain dried leaves of Moringa oleifera.
[0087] (2) Alkali treatment: Mix Moringa oleifera leaf dry powder and deionized water at a material-to-liquid ratio of 1:25g / mL evenly, add sodium hydroxide to adjust the pH to 9.0, keep the temperature at 55°C, and stir at 180r / min for 60min to obtain a suspension Liquid 1.
[0088] (3) Protease enzymatic hydrolysis: add protease to the suspension 1 at the rate of 8% dry leaf powder of Moringa oleifera, in which the amount of papain accounts for 90% of the total enzyme amount, and the amount of NS37071 alkaline protease accounts for 10% of the total enzyme amount , constant temperature at 55°C, stirring at 180r / min, enzymatic hydrolysis for 12h, and suspension 2 was obtained.
[0089] (4) Cellulase enzymatic hydroly...
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