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Fish scale collagen/sodium alginate composite porous bone tissue engineering scaffold and its preparation method and application

A fish scale collagen and tissue engineering scaffold technology, applied in the directions of tissue regeneration, connective tissue peptides, chemical instruments and methods, etc., can solve the problems of less use of biological materials, many steps and high production costs for fish scale collagen, and achieves Good cell adhesion and biocompatibility, low production cost, and the effect of promoting mineralization

Active Publication Date: 2021-05-28
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] From the above inventions, first of all, there are many steps to extract fish scale collagen, which are complicated and costly; secondly, the obtained fish scale collagen is rarely used for biological materials, especially for tissue engineering bone repair materials

Method used

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  • Fish scale collagen/sodium alginate composite porous bone tissue engineering scaffold and its preparation method and application
  • Fish scale collagen/sodium alginate composite porous bone tissue engineering scaffold and its preparation method and application
  • Fish scale collagen/sodium alginate composite porous bone tissue engineering scaffold and its preparation method and application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0063] A fish scale collagen / sodium alginate composite porous bone tissue engineering scaffold, comprising the following steps:

[0064] (1) Weigh a certain amount of bighead carp scales (from a certain vegetable market in Guangzhou), after cleaning, put them into a pulverizer to pulverize, and the particle size is 20 to 50 microns;

[0065] (2) Soak the pulverized fish scales in step (1) in 0.05M sodium chloride solution of 20 times the mass for 2 hours at a temperature of 20°C; then take out the fish scales and wash them with distilled water;

[0066] (3) Soak the fish scales treated in step (2) in 0.1M ethylenediaminetetraacetic acid solution of 30 times the mass for 6 hours at a temperature of 20°C; then take out the fish scales and wash them with distilled water;

[0067] (4) Soak the fish scales treated in step (3) in a 3wt% citric acid solution of 10 times the mass and add pepsin with a mass of 7wt% fish scales to extract for 8 hours, and the temperature is room tempera...

Embodiment 2

[0072] A sodium alginate / fish scale collagen composite porous bone tissue engineering scaffold, comprising the following steps:

[0073] (1) Weigh a certain amount of grass carp fish scales (from a vegetable market in Guangzhou), after cleaning, put them into a pulverizer to pulverize, and the particle size is 20-50 microns;

[0074] (2) Soak the pulverized fish scales in step (1) in 0.5M sodium chloride solution with a mass of more than 10 times for 5 hours at a temperature of 10°C; then take out the fish scales and wash them with distilled water;

[0075] (3) Soak the fish scales treated in step (2) in a 0.5M ethylenediaminetetraacetic acid solution with a mass of more than 50 times the mass for 3 hours at a temperature of 10°C; then take out the fish scales and wash them with distilled water;

[0076] (4) Soak the fish scales treated in step (3) in 3wt% citric acid solution of 20 times the mass and add pepsin whose mass is more than 7wt% of fish scales to extract for more t...

Embodiment 3

[0081] A sodium alginate / fish scale collagen composite porous bone tissue engineering scaffold, comprising the following steps:

[0082] (1) Weigh a certain amount of grass carp fish scales (from a vegetable market in Guangzhou), after cleaning, put them into a pulverizer to pulverize, and the particle size is 20-50 microns;

[0083] (2) Soak the pulverized fish scales in step (1) in 0.1M sodium chloride solution with a mass of more than 15 times for 3 hours at a temperature of 15°C; then take out the fish scales and wash them with distilled water;

[0084] (3) Soak the fish scales treated in step (2) in a 0.3M ethylenediaminetetraacetic acid solution with a mass of more than 40 times the mass for 5 hours at a temperature of 15°C; then take out the fish scales and wash them with distilled water;

[0085] (4) Soak the fish scales treated in step (3) in 15 times the mass of 3wt% citric acid solution and add pepsin whose mass is more than 7wt% of fish scales to extract for more t...

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Abstract

The invention discloses a fish scale collagen / sodium alginate composite porous bone tissue engineering scaffold, a preparation method and application thereof. The method comprises the following steps: (1) soaking the pulverized fish scales in sodium chloride solution, and then washing them with water to obtain fish scales A; (2) soaking the fish scales A in ethylenediaminetetraacetic acid solution, and then washing them with water clean to obtain fish scale B; (3) soak fish scale B in citric acid solution, and add protease for extraction to obtain mixed solution C; (4) adjust the pH value of mixed solution C to 11-12, and then place it for 6- Dialyze for 8 hours to obtain a fish scale collagen solution; (5) add sodium alginate solution to the fish scale collagen solution, mix well and then freeze-dry to obtain a fish scale collagen / sodium alginate composite porous bone tissue engineering scaffold. The scaffold has good cell attachment, biocompatibility and biodegradability, and can effectively promote mineralization.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and tissue engineering, and in particular relates to a fish scale collagen / sodium alginate composite porous bone tissue engineering scaffold and its preparation method and application. Background technique [0002] Collagen has good biological activity and can effectively promote cell growth, so it has a wide range of applications in the field of biomedicine, such as the preparation of tissue engineering scaffolds. Collagen is usually obtained from animals, such as epidermis and hoof tendon, etc. However, because the use of terrestrial animal-derived collagen is easy to cause cross-species virus infection, it may cause patients to be infected with diseases such as foot-and-mouth disease and mad cow disease, and cause harm to human society. Therefore, Europe and the United States Japan, Japan and other countries have successively issued regulations to restrict the use of extracts from mammalia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/24A61L27/20A61L27/50C07K14/78C12P21/06
CPCA61L27/20A61L27/24A61L27/50A61L27/56A61L2430/02C07K14/78C12P21/06C08L5/04
Inventor 敖宁建林越威张丽叶开均商玉莲谢浩诗李洲李日旺
Owner JINAN UNIVERSITY
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