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Culture method of acidophilic thiobacillus ferrooxidans

A technology of Thiobacillus ferrooxidans and ferrous oxide is applied in the biological field, which can solve the problems of slowing ferrous oxidation rate, speeding up biological leaching, difficulty in reaction solution, etc. The effect of low investment and operating costs and simple process

Inactive Publication Date: 2018-11-13
NANJING UNIV OF TECH
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Problems solved by technology

Finally, when Fe 2+ When it is too high, it will inhibit the activity of acidophilic Thiobacillus ferrooxidans, so that the oxidation rate of ferrous iron will be slowed down, and the conversion rate will be low, which will hinder the preparation of high-concentration Fe 3+ The reaction solution caused difficulties, while the high concentration of Fe 3+ It is necessary for the preparation of biopolymer iron and biological desulfurization, and it is also necessary for accelerating bioleaching

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preparation example Construction

[0029] The Fe-free 2+ Preparation method of 9K medium:

[0030] 3g / L (NH 4 ) 2 SO 4 , 0.5g / L MgSO 4 ·7H 2 O, 0.5g / L K 2 HPO 4 , 0.1g / L KCl, 0.01g / LCa (NO 3 ) 2 , adjust the pH to 1.8.

Embodiment 1

[0032] A method for cultivating acidophilic Thiobacillus ferrooxidans, comprising the steps of:

[0033] (1) Preparation of seed solution: prepare pH 1.8, containing 4.5g / L Fe 2+ Take a sterilized 250mL Erlenmeyer flask and add 90mL of sterilized 9K liquid medium containing 4.5g / L Fe 2+ 9K liquid medium (pH=1.8), inoculate 10mL of the initial bacterial liquid of acidophilic Thiobacillus ferrooxidans, and transfer it to a constant temperature shaker at 30°C and 180r / min for cultivation until the bacterial liquid turns from light green to dark to red Brown and the oxidation-reduction potential reaches 560mV, and the seed liquid is obtained;

[0034] (2) Expanded culture: Take 4 sterilized 500mL conical flasks, add 225mL of sterilized 4.5g / L Fe 2+ 9K liquid culture medium (pH=1.8), inoculate 25mL of the above seed liquid, and cultivate in a constant temperature shaker at 30°C and 180r / min. After 60h, the expanded culture is completed to obtain the bacterial liquid;

[0035] (3...

Embodiment 2

[0040] A method for cultivating acidophilic Thiobacillus ferrooxidans, comprising the steps of:

[0041] (1) Preparation of seed solution: prepare pH 1.8, containing 4.5g / L Fe 2+ Take a sterilized 250mL Erlenmeyer flask and add 90mL of sterilized 9K liquid medium containing 4.5g / L Fe 2+ 9K liquid medium (pH=1.8), inoculate 10mL of the initial bacterial liquid of acidophilic Thiobacillus ferrooxidans, and transfer it to a constant temperature shaker at 30°C and 180r / min for cultivation until the bacterial liquid turns from light green to dark to red Brown and the oxidation-reduction potential reaches 560mV, and the seed liquid is obtained;

[0042] (2) Expanded culture: Take 4 sterilized 500mL conical flasks, add 225mL of sterilized 4.5g / L Fe 2+ 9K liquid culture medium (pH=1.8), inoculate 25mL of the above seed liquid, and cultivate in a constant temperature shaker at 30°C and 180r / min. After 60h, the expanded culture is completed to obtain the bacterial liquid;

[0043] (3...

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Abstract

The invention relates to a culture method of acidophilic thiobacillus ferrooxidans. In a first stage, high-activity and high-concentration acidophilic thiobacillus ferrooxidans is obtained by ordinaryshake cultivation and continuous passage; in a second stage, the culture is continued under the synergistic effect of applied potential, and during this period, the change of cathode potential in a culture system is monitored, timing sampling is carried out, a ferrous oxidation rate, bacteria concentration and redox potential are periodically tested, and an electrochemically stimulated acidophilic Thiobacillus ferrooxidans solution is subjected to transferring passage to obtain a strain with high density and a high ferrous oxidation rate. The innovative culture method has low operating cost and wide application value.

Description

technical field [0001] The invention relates to a method for cultivating acidophilic Thiobacillus ferrooxidans, belonging to the field of biotechnology. Background technique [0002] Microorganisms of the acidophilic Thiobacillus ferrooxidans genus Prokaryotes, Chemotrophic Prokaryotes, Bacteria, Sulfurbacteriaceae, Thiobacillus. A single bacterium is short rod-shaped, 1 to several microns long and 0.5 microns wide, with two blunt round segments, flagella, and active movement. It belongs to Gram-negative bacteria and mainly uses CO 2 As a carbon source, it absorbs inorganic nutrients such as nitrogen and phosphorus to synthesize its own cells. Acidophilic Thiobacillus ferrooxidans is a good chemoautotrophic bacterium, its optimum growth pH is 1.5-2.5, and its optimum growth temperature is 28-35°C. [0003] With the continuous in-depth research on the physiological and biochemical characteristics of acidophilic Thiobacillus ferrooxidans, it has been found that this type of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 周俊吴平仉丽娟贾红华
Owner NANJING UNIV OF TECH
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