Strain capable of degrading waste feathers, screening method and application thereof

A screening method and feather technology, applied in the field of microorganisms, can solve the problems of damage to the nutritional value of feather meal, loss of nutrients, pollution of the environment, etc., and achieve the effects of improving absorbability, avoiding loss of amino acids, and high degradation efficiency.

Active Publication Date: 2018-11-13
XINYANG NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can promote the digestion and utilization of feathers by animals to a certain extent, there are inherent defects in these processes, such as high energy consumption and environmental pollution.
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  • Strain capable of degrading waste feathers, screening method and application thereof
  • Strain capable of degrading waste feathers, screening method and application thereof

Examples

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Example Embodiment

[0029] Example 1: Screening and molecular identification of bacterial strains that can efficiently degrade discarded feathers

[0030] Taking four months of air-dried tea garden soil as a sample, through the steps of preliminary screening, re-screening, obtaining pure culture, quantitative determination of feather degradation ability, molecular identification, and strain preservation, a high-efficiency feather degrading strain Bacillus cereus FDB-18B was obtained. The specific screening steps are as follows:

[0031] (1) Sampling: Weigh 4 g of the tea garden soil sample that has been air-dried for 4 months, dissolve it in 40 ml of sterilized sterile water, vortex and shake it, and let it stand for 5 minutes to obtain a soil suspension;

[0032] (2) Preliminary screening: Take 1ml of the soil suspension and add it to the primary screening medium (just after sterilization, the temperature is not lower than 90°C), and incubate at 37°C and 150rpm for 2 days to obtain a mixed solution;

[...

Example Embodiment

[0044] Example 2: Application of bacterial strain Bacillus cereus FDB-18B that can efficiently degrade discarded feathers

[0045] Take out the frozen strain tube from the refrigerator at -80℃, immediately put it in 37℃ warm water to make it melt quickly, and inoculate 10μl in 10ml sterilized LB liquid medium, 37℃(150rpm) Incubate in a shaker overnight (about 15h). According to the ratio of 1:50-100, inoculate the bacterial solution into a new Erlenmeyer flask containing 50ml of LB liquid medium, and cultivate to the logarithmic phase in a shaker at 37°C (150rpm). The culture shall be adjusted to the initial cell concentration Inoculate 0.007-0.06 (OD600) into a fermentation medium containing intact feathers, continue to culture at 37℃ at 150 rpm to degrade the feathers, and take photos to record the changes in the complete feathers after 24 hours of degradation. The results are shown figure 1 .

[0046] The specific components of the feather fermentation medium are: sodium chlor...

Example Embodiment

[0048] Example 3: Optimization of the application of the bacterial strain Bacillus cereus FDB-18B that can efficiently degrade discarded feathers

[0049] Take out the frozen strain tube from the refrigerator at -80℃, immediately place it in warm water at 37℃ to make it melt quickly, and inoculate 10μl in 10ml sterilized LB liquid medium at 37℃, 150rpm Incubate in a shaker overnight (about 15h). According to the ratio of 1:50-100, inoculate the bacterial solution into a new Erlenmeyer flask containing 50ml of LB liquid medium, and cultivate to the logarithmic phase in a shaker at 37°C (150rpm). The culture shall be adjusted to the initial cell concentration Inoculate 0.015 (OD600) into the fermentation medium containing intact feathers (addition of soluble starch concentration of 0, 1.25%, 2.5%, 5%, 10%, weight / volume ratio, among which, to add soluble starch concentration of 0 As a control group, the rest are experimental groups), continue to culture at 37°C and 150 rpm to degr...

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Abstract

The invention relates to a strain capable of degrading waste feathers and belongs to the technical field of microorganisms. The strain is Bacillus cereus FDB-18B, deposited in the China Center for Type Culture Collection on June 13, 2018 with the deposit number of CCTCC NO. 2018365. The strain is obtained from the soil of a tea garden after air drying, and can efficiently degrade the waste feathers to obtain amino acids and polypeptides that can be used as feed additives or for farmland irrigation to achieve comprehensive utilization of biomass resources. At the same time, the pollution of poultry breeding to the environment can be reduced. In addition, the strain can produce amylase, and can simultaneously achieve comprehensive treatment of wastewater generated from processing of the waste feathers and starch-containing food. Due to high degradation capability and short fermentation cycle, the strain keeps the protein, polypeptide and amino acid components to the greatest extent, andthe nutritional value and the utilization rate of the feed additives can be improved.

Description

technical field [0001] The invention relates to a bacterial strain capable of degrading discarded feathers, belonging to the technical field of microorganisms. Background technique [0002] Feather waste from poultry production is considerable. It is estimated that in the case of China, the annual output can also be as high as 1 million tons. Although the main component of feathers is protein, the content of keratin is as high as about 90%, so it is difficult to be degraded, and it is not easy to be digested by the digestive enzymes in the intestinal tract of animals. Therefore, it has not been effectively used for a long time. This not only causes the waste of these available biological resources, but also brings about the problem of environmental pollution. [0003] With the improvement of Chinese people's living standards in recent years, the proportion of animal food has been increasing, and the scale of poultry farming has also been further expanded, making the resul...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/12C12R1/085
CPCA23K10/12C12N1/20C12N1/205C12R2001/085
Inventor 王天文梁辰高婉茹高倩罗欣琪
Owner XINYANG NORMAL UNIVERSITY
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