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Instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement and preparation method thereof

A technology of dietary supplement and phospholipid type, which is applied in the field of instant type egg yolk oligopeptide-phospholipid type DHA double-effect dietary supplement and its preparation field, can solve the problem of the preparation of egg yolk oligopeptide-phospholipid type DHA double-effect dietary supplement which has not yet been seen methods, etc., to achieve the effects of high biological activity and intestinal absorption and utilization rate, high product yield, good water solubility and solubility stability

Inactive Publication Date: 2018-11-16
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant reports on egg yolk oligopeptide-phospholipid DHA double-effect dietary supplement and its preparation method

Method used

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  • Instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement and preparation method thereof
  • Instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement and preparation method thereof
  • Instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Insert the strain of Schizochytrium SD006 (the strain is described in the patent document No. 201410203041.0) stored in the glycerol tube into a 500mL shake flask equipped with 100mL seed medium (see Table 1), at 30 Cultivate on a shaker at ℃ for 24 hours to obtain first-grade seeds; put the first-grade seeds into a 5L fermenter equipped with 1L of seed medium (see Table 1), and culture on a shaker at 30°C for 24 hours to obtain second-grade seeds; ferment to 50L Add 20L of fermentation medium (see Table 2) into the tank, and insert activated secondary seed liquid. During the fermentation process, the temperature was controlled at 28° C., and the fermentation was carried out for 60 hours. During the fermentation process, 50% (w / v) corn steep liquor solution was fed, and the pH value of the system was maintained at 6.5 by feeding 2M NaOH or 14% citric acid solution.

[0043] Table 1 Schizochytrium produces the seed medium formula of DHA

[0044]

[0045] The ferm...

Embodiment 2

[0066] 1) The Schizochytrium SD006 (201410203041.0) strain stored in a glycerol tube was inserted into a 500mL shake flask containing 100mL seed medium (see Table 1), and cultured on a shaker at 30°C for 24h to obtain first-grade seeds; The primary seeds were placed in a 5L fermenter with 1L of seed medium (see Table 1), and cultured on a shaker at 30°C for 24 hours to obtain secondary seeds; 20L of fermentation medium was added to the 50L fermenter (see Table 3) , into the activated secondary seed solution. During the fermentation process, the temperature was controlled at 35° C., and the fermentation was carried out for 80 hours. During the fermentation process, 50% (w / v) corn steep liquor solution was fed, and the pH value of the system was maintained at 6.5 by feeding 2M NaOH or 14% citric acid solution.

[0067] Table 3 The fermentation medium formula of Schizochytrium producing DHA

[0068]

[0069]

[0070] 2) After the fermentation is finished, the Schizochytrium...

Embodiment 3

[0079] 1) Insert the Schizochytrium SD006 (201410203041.0) strain stored in a glycerol tube into a 500mL shake flask containing 100mL seed medium (see Table 1), and culture it on a shaker at 30°C for 24h to obtain first-grade seeds; Put the primary seeds into a 5L fermenter with 1L of seed medium (see Table 1), and culture them on a shaker at 30°C for 24 hours to obtain secondary seeds; add 20L of fermentation medium to the 50L fermenter (see Table 4) , into the activated secondary seed solution. During the fermentation process, the temperature was controlled at 30° C., and the fermentation was carried out for 80 hours. During the fermentation process, 50% (w / v) corn steep liquor solution was fed, and the pH value of the system was maintained at 6.5 by feeding 2M NaOH or 14% citric acid solution.

[0080] The fermentation medium formula of table 4 Schizochytrium producing DHA

[0081]

[0082] 2) After the fermentation is finished, the Schizochytrium fermented liquid in t...

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Abstract

The invention belongs to the technical field of food biology, and particular relates to an instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement and a preparation method thereof. The preparation method comprises the following steps of performing high-density fermentation so as to obtain microalgae powder of which the DHA content is 25-35%, adding the DHA microalgae powderto a laying hen ration to obtain strengthened eggs of which the DHA content is 200-400mg / 100g, performing pretreatment and continuous hydrolysis on DHA strengthened yolk liquid to obtain DHA strengthened yolk hydrolysate, and performing vacuum concentration refined fishy smell elimination, emulsifying, homogenizing, spray drying and boiling granulation on the yolk hydrolysate so as to obtain theinstant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement. The method disclosed by the invention has the advantages of being simple in technology, free from environmental pollution, high in product yield and the like; and the total protein content of the instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement is higher than 45%, the proportion of yolkoligopeptide components in the total protein is higher than 50%, the content of DHA in the product namely the instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement is 1-3%, the maltodextrin content is 5-25%, and the instant yolk oligopeptide-phosphatide type DHA double-effect dietary supplement is particularly suitable for nutrient requirement crowds of sub-health crowds,babies at the growth and development period, women at the pregnancy period or the lactation period, postoperative patients, crowds suffering from disturbance of digestion and absorption, old people suffering from slow metabolism, and the like to eat.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a fast-food egg yolk oligopeptide-phospholipid DHA double-effect dietary supplement and a preparation method thereof. Background technique [0002] Dietary supplements, also known as nutritional supplements, are dietary supplements that can be composed of amino acids, polyunsaturated fatty acids, minerals and vitamins, or only one or more dietary ingredients. For sub-healthy people, infants and young children during growth and development, pregnant or breastfeeding women, patients after surgery, people with digestive and absorption disorders, and the elderly with slow metabolism, etc., fast-food dietary supplements can quickly and effectively supplement nutrients and improve the health of the above-mentioned people. Absorption of certain nutrients. The development of fast-food multi-effect dietary supplements is one of the focuses of the food industry today. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L33/125A23L33/115A23L5/20A23J1/08A23K50/75A23K10/12
CPCA23J1/08A23K10/12A23K50/75A23L5/20A23L33/115A23L33/125A23L33/18A23V2002/00A23V2200/30A23V2250/55A23V2250/5114
Inventor 崔球王彦超宋晓金兰传增冯银刚刘亚君
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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