Preparation method for composite photothermal material and application thereof
A technology of thermal materials and composite light, which is applied in the field of biomedicine to achieve the effect of reducing side effects and killing efficiently
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Embodiment 1
[0030] This embodiment relates to the preparation method of the DEPt-COOH composite material, which specifically includes the following steps:
[0031] Synthesis of DEPt-COOH: Add 0.476mL potassium hexachloroplatinate solution (20mM) to 0.55mLG4.5-COONa PAMAM (0.346mM) solution (pH=2), stir for 3min and adjust the pH of the solution with 0.1M sodium hydroxide Adjusted to 9.16. After stirring for 12 h in the dark, 0.476 mL of sodium borohydride (200 mM) was added rapidly. After 2 hours, the solution was transferred into a dialysis bag with a molecular weight cut-off of 3500 Da, dialyzed 10 times with PBS buffer (pH=9), collected and stored at 4°C.
Embodiment 2
[0041] This embodiment relates to DEPt-COOH, DEPt-NH 2 , In vitro bone targeting performance of DEPt-AC, and evaluation of photothermal killing effect of cells
[0042] Using hydroxyapatite bone slices as the experimental object, the bone targeting ability of the above three composite materials was observed, and the results showed that the ability of DEPt-COOH to bind hydroxyapatite was far superior to that of the other two materials (such as Figure 4 shown). When breast cancer cells MDA-MB-231-Luc were used as experimental cells to detect the photothermal killing effect of the above three composite materials on tumor cells, it was found that: DEPt-NH 2 , DEPt-AC, DEPt-COOH three complexes all have excellent tumor cell killing ability, and the ability is almost the same (such as Figure 5 shown). The above data show that DEPt-COOH has excellent hydroxyapatite binding ability (calcium ion binding ability), and the photothermal conversion performance and tumor cell killing a...
Embodiment 3
[0044] This example relates to an animal experiment on nude mice, evaluating the effect of photothermal therapy on a bone tumor model
[0045] Animal models were established using MDA-MB-231 cells stably expressing luciferase. MDA-MB-231-luc was transfected with a plasmid co-expressing luciferase gene and kanamycin (G418) resistance gene with Lipofectamine 2000 (Invitrogen, Carlsbad, CA), and G418 was used as pressure selection drug. The bioluminescent activity of cells and tumors was detected by Xenogen IVIS-200 (Caliper Life Sciences, Hopkinton, MA) after adding D-luciferin. 5-week-old Balb / c nude female mice were selected as experimental animals, and the mice were kept in the SPF animal room of East China Normal University. Adjust the MDA-MB-231-luc cell concentration to 1 × 10 7 / mL, resuspended with PBS buffer, and then the cell resuspension (20 μL) was injected percutaneously into the right tibia of the mouse (the right tibia is the tumor-bearing side). After successf...
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